Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IM698_RS14680 Genome accession   NZ_CP063682
Coordinates   3292399..3294093 (+) Length   564 a.a.
NCBI ID   WP_409574887.1    Uniprot ID   -
Organism   Vibrio splendidus strain ED144     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3287399..3299093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM698_RS14660 (IM698_14640) pilM 3289589..3290614 (+) 1026 WP_260809858.1 type IV pilus assembly protein PilM Machinery gene
  IM698_RS14665 (IM698_14645) pilN 3290598..3291212 (+) 615 WP_260809859.1 PilN domain-containing protein Machinery gene
  IM698_RS14670 (IM698_14650) pilO 3291205..3291834 (+) 630 WP_248388986.1 type 4a pilus biogenesis protein PilO Machinery gene
  IM698_RS14675 (IM698_14655) pilP 3291821..3292339 (+) 519 WP_248391098.1 pilus assembly protein PilP Machinery gene
  IM698_RS14680 (IM698_14660) pilQ 3292399..3294093 (+) 1695 WP_409574887.1 type IV pilus secretin PilQ Machinery gene
  IM698_RS14685 (IM698_14665) aroK 3294270..3294788 (+) 519 WP_009848907.1 shikimate kinase AroK -
  IM698_RS14690 (IM698_14670) aroB 3294813..3295901 (+) 1089 WP_248373112.1 3-dehydroquinate synthase -
  IM698_RS14695 (IM698_14675) - 3295981..3297498 (+) 1518 WP_260809861.1 SPOR domain-containing protein -
  IM698_RS14700 (IM698_14680) - 3297568..3298416 (+) 849 WP_248373100.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61503.98 Da        Isoelectric Point: 4.3042

>NTDB_id=496238 IM698_RS14680 WP_409574887.1 3292399..3294093(+) (pilQ) [Vibrio splendidus strain ED144]
MSKALQGFAVSVMLVFSVFSYAESLPNKLENIDFRVNKDKDAVIIIELATTTAVVDVQRAQEGLSIELINTEVDDDKLYL
LDVKDFATLVEGIEVYKETPSTRLLATINNDYQYEYNLKGRFIEVVISKPVIDEATKEKSVLEKEDKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEKSRLEEELG
ELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQIEARIVTV
TEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSAITPYDDDSGSSVIDDYLNVNLGATSPNASSIAFQVAKLGSDTLLDL
ELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRP
GQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGTLFRRSYENVGKSELLIFVTPK
VVIQ

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=496238 IM698_RS14680 WP_409574887.1 3292399..3294093(+) (pilQ) [Vibrio splendidus strain ED144]
GTGAGCAAGGCTCTACAAGGTTTTGCTGTGTCTGTGATGTTGGTTTTTAGCGTATTCAGTTATGCAGAAAGCCTACCAAA
TAAATTAGAGAATATTGATTTTAGGGTTAATAAGGATAAAGACGCCGTCATTATTATTGAGCTAGCCACCACCACTGCGG
TGGTCGATGTGCAGCGTGCTCAGGAAGGGTTAAGTATCGAACTCATAAATACTGAGGTTGACGATGACAAGCTCTACCTA
CTGGATGTGAAGGATTTCGCTACCTTGGTTGAAGGCATTGAAGTCTATAAAGAGACACCCAGCACTCGGCTTCTCGCGAC
GATTAACAATGACTATCAGTATGAATACAACCTAAAGGGGCGCTTTATTGAGGTGGTGATCAGCAAACCCGTTATCGATG
AAGCGACCAAAGAAAAAAGCGTTCTAGAGAAAGAGGATAAGCTGATATCGATTAACTTCCAAGACATCCCAGTACGAAAT
GTCCTACAGTTGATTGCCGACTACAACGACTTCAATCTCGTCGTGTCTGATTCGGTAGCCGGAAACCTGACGTTACGCTT
AGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGTTTGGATAAGCGTGTTGATGGCAATGTCA
TCTTGGTTGCGCCGAAAGCGGAACTCGATCTTCGAGAGCAGCAAGCGCTCGAGAAATCGCGTTTAGAAGAGGAGTTAGGG
GAGCTTAAATCGGAAATCATCAAGATTAACTTTGCAAAAGCCACCGACATTGCTGACATGATTGGCGGTGAAGGCGCAGT
CAGCATGTTGTCCGATCGTGGTTCGATTACTATTGATGAGAGAACCAATTCACTGCTCATTCGTGAATTGGAAGAAAACA
TTGCGGTGATACGAGGCATCATTGAATCGCTAGATATTCCTGTTAAGCAAGTACAGATAGAGGCGCGTATTGTAACCGTC
ACTGAGGGTAATCTTGATGAGCTTGGCGTTCGCTGGGGCGTTTCCTCAACCAACGGAAGCTTCACTGTTGGTGGTTCAAT
TGAAGGGAACCACCCATCAGCGATTACGCCATATGACGACGATAGCGGCAGCAGCGTGATAGATGACTATCTCAACGTAA
ACTTAGGGGCAACGTCACCGAACGCTTCGAGTATTGCATTTCAGGTGGCCAAGTTGGGGTCTGATACATTGCTCGATCTT
GAGCTCTCAGCACTGCAACAAGAGTCGAAAGCTGAGATCATTTCGAGCCCACGCTTAATTACCACCAATAAAAAGCCGGC
TTATATTGAGCAAGGTACTGAAATCCCTTACTTAGAGTCCTCTTCAAGTGGTGCAACGTCGGTCGCATTTAAGAAAGCGG
TATTGAGTCTCAAGGTGACGCCGCAGATAACGCCCGACAATCGCTTGGTGTTGGATTTGAGTGTGACTCAAGATAGACCA
GGGCAAGTGGTAAAAACCGGAACTGGAGAAGCTGTGGCGATTGATACTCAACGTATTGGCACGCAAGTGCTTGTTAATAA
TGGTGAAACTGTCGTGCTTGGTGGGATATTCCAACACAGCGTGAGCAGTACTGTGGATAAAGTGCCTCTGTTGGGAGACC
TGCCAGTTTTGGGTACATTGTTCCGTCGCAGCTATGAAAATGTGGGTAAAAGTGAACTACTTATTTTTGTCACACCCAAA
GTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Bacterial type II and III secretion system protein (Secretin)

(404-562)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

79.928

98.05

0.784
  pilQ Vibrio cholerae strain A1552

80.073

96.986

0.777
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.073

96.986

0.777
  pilQ Legionella pneumophila strain ERS1305867

42.857

96.809

0.415
  pilQ Legionella pneumophila strain Lp02

42.857

96.809

0.415
  pilQ Pseudomonas aeruginosa PAK

39.93

100

0.404
  comQ Acinetobacter baylyi ADP1

39.502

99.645

0.394
  pilQ Acinetobacter baumannii strain A118

37.133

100

0.381
  pilQ Acinetobacter baumannii D1279779

37.133

100

0.381