Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IM698_RS14660 Genome accession   NZ_CP063682
Coordinates   3289589..3290614 (+) Length   341 a.a.
NCBI ID   WP_260809858.1    Uniprot ID   -
Organism   Vibrio splendidus strain ED144     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3284589..3295614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM698_RS14640 (IM698_14625) - 3284920..3285648 (-) 729 WP_017061133.1 glutathione peroxidase -
  IM698_RS14645 - 3285693..3285872 (-) 180 WP_248373125.1 hypothetical protein -
  IM698_RS14650 (IM698_14630) oxyR 3285871..3286764 (+) 894 WP_248373123.1 DNA-binding transcriptional regulator OxyR -
  IM698_RS14655 (IM698_14635) - 3286876..3289437 (-) 2562 WP_260809857.1 penicillin-binding protein 1A -
  IM698_RS14660 (IM698_14640) pilM 3289589..3290614 (+) 1026 WP_260809858.1 type IV pilus assembly protein PilM Machinery gene
  IM698_RS14665 (IM698_14645) pilN 3290598..3291212 (+) 615 WP_260809859.1 PilN domain-containing protein Machinery gene
  IM698_RS14670 (IM698_14650) pilO 3291205..3291834 (+) 630 WP_248388986.1 type 4a pilus biogenesis protein PilO Machinery gene
  IM698_RS14675 (IM698_14655) pilP 3291821..3292339 (+) 519 WP_248391098.1 pilus assembly protein PilP Machinery gene
  IM698_RS14680 (IM698_14660) pilQ 3292399..3294093 (+) 1695 WP_409574887.1 type IV pilus secretin PilQ Machinery gene
  IM698_RS14685 (IM698_14665) aroK 3294270..3294788 (+) 519 WP_009848907.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38274.76 Da        Isoelectric Point: 6.4182

>NTDB_id=496234 IM698_RS14660 WP_260809858.1 3289589..3290614(+) (pilM) [Vibrio splendidus strain ED144]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQGHGLLNVWQLASRLYPDKKNWLLVDVGIDQTSLGIIPQNSAPFYKDIAFGTQDLRSSDDPSDIESVFGTMEETH
QFIVNLIEKLKRQLQLYSSVNALQSISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=496234 IM698_RS14660 WP_260809858.1 3289589..3290614(+) (pilM) [Vibrio splendidus strain ED144]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGGAACGTCGCCATTTCAGTACCTGATAACACC
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
CCAGTCTCCCTTTCCTATTGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGATCGACAA
GCAGTTATCAGGTGTATGCCACTCGTAAGGAAGTGGTCGATAGCCGCGCTGATGCATTAACCAAGGCTGGTTTTAAACCT
GTGGTGGTGGATACGCAGGGGCACGGGCTGCTTAATGTCTGGCAATTGGCTTCGCGCTTGTATCCCGATAAGAAGAACTG
GTTGCTGGTGGATGTTGGAATTGACCAAACCTCGCTAGGGATTATTCCGCAGAATTCGGCGCCTTTCTATAAAGACATCG
CATTTGGCACTCAAGATCTTCGCAGCTCAGATGACCCGAGTGATATCGAGAGCGTGTTCGGTACTATGGAAGAGACACAT
CAATTCATCGTTAACCTGATTGAGAAGCTCAAGCGTCAATTGCAGCTCTATTCGTCAGTCAATGCACTTCAGTCTATTTC
AGGGATCTGGCTAATGGGTGAGGGAGCGAGCATCCCGATGGTCACTGAAGAGCTAGAGCGCCATTTTCAGTTGAGCTGTG
AGTCGCTCAACCCTTTATCTCTGTTTGAGAACAAAGTAGCGAAGCGACGTCGACTGCCAATGGACTGGCAACACTTTGGC
ATTGCCGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

55.425

100

0.554
  pilM Vibrio campbellii strain DS40M4

48.397

100

0.487