Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IM698_RS14670 Genome accession   NZ_CP063682
Coordinates   3291205..3291834 (+) Length   209 a.a.
NCBI ID   WP_248388986.1    Uniprot ID   -
Organism   Vibrio splendidus strain ED144     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3286205..3296834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM698_RS14655 (IM698_14635) - 3286876..3289437 (-) 2562 WP_260809857.1 penicillin-binding protein 1A -
  IM698_RS14660 (IM698_14640) pilM 3289589..3290614 (+) 1026 WP_260809858.1 type IV pilus assembly protein PilM Machinery gene
  IM698_RS14665 (IM698_14645) pilN 3290598..3291212 (+) 615 WP_260809859.1 PilN domain-containing protein Machinery gene
  IM698_RS14670 (IM698_14650) pilO 3291205..3291834 (+) 630 WP_248388986.1 type 4a pilus biogenesis protein PilO Machinery gene
  IM698_RS14675 (IM698_14655) pilP 3291821..3292339 (+) 519 WP_248391098.1 pilus assembly protein PilP Machinery gene
  IM698_RS14680 (IM698_14660) pilQ 3292399..3294093 (+) 1695 WP_409574887.1 type IV pilus secretin PilQ Machinery gene
  IM698_RS14685 (IM698_14665) aroK 3294270..3294788 (+) 519 WP_009848907.1 shikimate kinase AroK -
  IM698_RS14690 (IM698_14670) aroB 3294813..3295901 (+) 1089 WP_248373112.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24345.82 Da        Isoelectric Point: 4.5774

>NTDB_id=496236 IM698_RS14670 WP_248388986.1 3291205..3291834(+) (pilO) [Vibrio splendidus strain ED144]
MANLQNRISWQNRMSFQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDDELQQMKQQEQTLKTTLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNSLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=496236 IM698_RS14670 WP_248388986.1 3291205..3291834(+) (pilO) [Vibrio splendidus strain ED144]
ATGGCTAACCTTCAAAATAGGATTAGTTGGCAAAACAGGATGAGTTTTCAGGATCTTGATGTTGATGAGATCACCGAATG
GCCATTGCTGCCTCAGCTTCTCGTGATCCTGGTGTTGATAGTGTTAATCCAAGGTGTCGGCACTTGGCTTTATGTGCTGC
CTCTCGACGATGAACTGCAACAGATGAAGCAACAAGAACAAACCTTAAAGACGACCTTGCGAATAAAGGCTAATAAGGTG
GCAGCCTTACCTAAGTTGCAGAGCCAGTTGGATGAGCTAACCAGTCGCTACGATTATCTGTTAGAGCAACTGCCCGTTCA
AAAAGAGCTGGCCAGTATGTTGGCGTCTGTGAATGAACTCGGCTTGGATAATTCGCTGACCTTTACTCGAATCGATTGGG
GACAAAAGCAGAATAAGGAGTTTCTCTATCGTTTACCGCTCAATATCGAGCTGACGGGTGACTATCACGAGATCGGCGAT
TTCTCGGCTGCTATCGCCAAACTGCCGCGCATCATTAGTTTTGATGATGTGAATTGGCAGCGAGTCAGCCAAGAAAGCAG
CACGCTGCACTTTAGGGTTCGAGCTTATACCTACCAGTTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.648

92.344

0.699
  pilO Vibrio campbellii strain DS40M4

71.809

89.952

0.646