Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INP97_RS00255 Genome accession   NZ_CP063113
Coordinates   52208..52651 (+) Length   147 a.a.
NCBI ID   WP_065243023.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C147_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47208..57651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP97_RS00225 (INP97_00225) tadA 47423..47941 (-) 519 WP_197562681.1 tRNA adenosine(34) deaminase TadA -
  INP97_RS00230 (INP97_00230) - 47951..48802 (-) 852 WP_197562682.1 thymidylate synthase -
  INP97_RS00235 (INP97_00235) lgt 48813..49622 (-) 810 WP_197562683.1 prolipoprotein diacylglyceryl transferase -
  INP97_RS00240 (INP97_00240) - 49631..50425 (-) 795 WP_197562684.1 sulfite exporter TauE/SafE family protein -
  INP97_RS00245 (INP97_00245) rppH 50556..51018 (-) 463 Protein_48 RNA pyrophosphohydrolase -
  INP97_RS00250 (INP97_00250) ampD 51514..52065 (-) 552 WP_197562686.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP97_RS00255 (INP97_00255) pilA 52208..52651 (+) 444 WP_065243023.1 prepilin peptidase-dependent pilin Machinery gene
  INP97_RS00260 (INP97_00260) pilB 52658..54037 (+) 1380 WP_197562687.1 GspE/PulE family protein Machinery gene
  INP97_RS00265 (INP97_00265) pilC 54046..55269 (+) 1224 WP_197562688.1 type II secretion system F family protein Machinery gene
  INP97_RS00270 (INP97_00270) pilD 55266..55943 (+) 678 WP_197562689.1 prepilin peptidase Machinery gene
  INP97_RS00275 (INP97_00275) coaE 56032..56652 (+) 621 WP_049369503.1 dephospho-CoA kinase -
  INP97_RS00280 (INP97_00280) yacG 56645..56851 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP97_RS00285 (INP97_00285) - 56848..57129 (+) 282 WP_014064065.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15167.42 Da        Isoelectric Point: 8.4674

>NTDB_id=492967 INP97_RS00255 WP_065243023.1 52208..52651(+) (pilA) [Haemophilus parainfluenzae strain M1C147_1]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNSPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=492967 INP97_RS00255 WP_065243023.1 52208..52651(+) (pilA) [Haemophilus parainfluenzae strain M1C147_1]
ATGAAACTGACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCGGCTATCTCCGAATTGTTGCAAGCATCTGCAC
CTTATAAATCTGATGTGGAGTTATGTGTCTATAGCACCAATTCCCCGACAAACTGTTCTGGTGGTTCAAATGGCATTGCA
GCAGACATCACTACTGCAAAAGGCTATGTTAAATCCATTACCACAAAATCGGGTGTAATTACGGTAACAGGAAATGGGGC
ATTGGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTTACCTGGACAACCGCCTGCCCAAGCA
ATGCGGATTTATTCCCTGCGGGTTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75.694

97.959

0.741
  pilA Haemophilus influenzae Rd KW20

73.611

97.959

0.721
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

40.411

99.32

0.401
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.054

100

0.361
  pilA Vibrio cholerae strain A1552

36.054

100

0.361
  pilA Vibrio cholerae C6706

36.054

100

0.361