Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP97_RS00260 Genome accession   NZ_CP063113
Coordinates   52658..54037 (+) Length   459 a.a.
NCBI ID   WP_197562687.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C147_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47658..59037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP97_RS00230 (INP97_00230) - 47951..48802 (-) 852 WP_197562682.1 thymidylate synthase -
  INP97_RS00235 (INP97_00235) lgt 48813..49622 (-) 810 WP_197562683.1 prolipoprotein diacylglyceryl transferase -
  INP97_RS00240 (INP97_00240) - 49631..50425 (-) 795 WP_197562684.1 sulfite exporter TauE/SafE family protein -
  INP97_RS00245 (INP97_00245) rppH 50556..51018 (-) 463 Protein_48 RNA pyrophosphohydrolase -
  INP97_RS00250 (INP97_00250) ampD 51514..52065 (-) 552 WP_197562686.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP97_RS00255 (INP97_00255) pilA 52208..52651 (+) 444 WP_065243023.1 prepilin peptidase-dependent pilin Machinery gene
  INP97_RS00260 (INP97_00260) pilB 52658..54037 (+) 1380 WP_197562687.1 GspE/PulE family protein Machinery gene
  INP97_RS00265 (INP97_00265) pilC 54046..55269 (+) 1224 WP_197562688.1 type II secretion system F family protein Machinery gene
  INP97_RS00270 (INP97_00270) pilD 55266..55943 (+) 678 WP_197562689.1 prepilin peptidase Machinery gene
  INP97_RS00275 (INP97_00275) coaE 56032..56652 (+) 621 WP_049369503.1 dephospho-CoA kinase -
  INP97_RS00280 (INP97_00280) yacG 56645..56851 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP97_RS00285 (INP97_00285) - 56848..57129 (+) 282 WP_014064065.1 GNAT family N-acetyltransferase -
  INP97_RS00290 (INP97_00290) rhlB 57220..58479 (+) 1260 WP_049369584.1 ATP-dependent RNA helicase RhlB -
  INP97_RS00295 (INP97_00295) mreD 58521..59009 (-) 489 WP_049364929.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52251.66 Da        Isoelectric Point: 6.5020

>NTDB_id=492968 INP97_RS00260 WP_197562687.1 52658..54037(+) (pilB) [Haemophilus parainfluenzae strain M1C147_1]
MAYQAVAKNGEIYHISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETTQLK
QLLQSLAPKANQIEEQTTFYHHSEDESTANLSNEDEPVIQLLNSIFETALQKNASDIHLETQSNGFLIRLRIDGVLQPQP
VISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQK
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQPHEIENSLLLVIAQRLVRKRCLKCGQHF
SYSCDCHQGYRGRIGVYQFLQCENNRYQTDFESLYQSALEKVKDRITDLIEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=492968 INP97_RS00260 WP_197562687.1 52658..54037(+) (pilB) [Haemophilus parainfluenzae strain M1C147_1]
ATGGCCTATCAAGCAGTCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGCTGCGTTACTTTGCGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCGGTGGATTCGCTGAATA
ATCTTGCGGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTGGTTGAACCGATTTTATTTGAAACGACTCAACTCAAG
CAACTTTTACAATCCCTTGCACCTAAAGCCAATCAAATAGAAGAACAAACGACGTTTTATCATCATTCAGAAGACGAAAG
CACGGCTAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAACAGTATTTTTGAAACAGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGTCAAATGGGTTCTTAATTCGTTTACGTATTGATGGTGTACTCCAACCTCAGCCG
GTAATCAGTAAATCGCTCGCCAATCGTGTAATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACATTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCTGTCCAACTAAGTTTTGCTGAGTTAGGTATGACGCAGAAG
CAACAAAGTCAATTTCAACACGCGCTCAGCCAACCGCAAGGTTTAATTCTGGTGACCGGCCCCACGGGAAGTGGAAAAAG
TATCTCACTTTATACCGCGCTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGCATCATTCAAACTCAAGTGAATCCGCAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGACGAAGAAAGTGCATTGATGGCATTAAGAGCCGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCGATTTCACGCTTACAACAATTAGGCATTCAACCTC
ATGAAATTGAAAACAGTCTCTTATTAGTTATCGCACAGAGATTGGTGCGTAAACGTTGCTTAAAGTGCGGACAACATTTT
AGTTATTCTTGCGATTGCCATCAGGGATATCGAGGTCGTATTGGTGTGTATCAATTTTTACAATGCGAAAATAACCGTTA
CCAAACTGATTTTGAGTCACTTTATCAAAGTGCGTTAGAAAAAGTAAAAGATCGCATCACCGATCTCATCGAAATTCAAC
GCGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)

Type II/IV secretion system protein (T2SSE)

(124-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.242

99.129

0.776
  pilB Haemophilus influenzae Rd KW20

77.363

99.129

0.767
  pilB Glaesserella parasuis strain SC1401

59.513

98.475

0.586
  pilB Vibrio cholerae strain A1552

41.875

100

0.438
  pilB Vibrio campbellii strain DS40M4

40.625

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.417

100

0.423
  pilB Acinetobacter baumannii D1279779

38.809

100

0.412
  pilB Legionella pneumophila strain ERS1305867

38.65

100

0.412
  pilB Acinetobacter baylyi ADP1

37.626

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.307

100

0.364
  pilF Neisseria gonorrhoeae MS11

38.928

93.464

0.364