Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IL983_RS19655 Genome accession   NZ_CP063047
Coordinates   4277024..4278241 (+) Length   405 a.a.
NCBI ID   WP_003161763.1    Uniprot ID   A0A3P3S6C0
Organism   Pseudomonas aeruginosa strain KC-Tt-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4273164..4285722 4277024..4278241 within 0


Gene organization within MGE regions


Location: 4273164..4285722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL983_RS19640 (IL983_19640) - 4273164..4274543 (-) 1380 WP_232095709.1 O-antigen ligase family protein -
  IL983_RS19645 (IL983_19645) - 4274625..4275089 (-) 465 WP_021205141.1 pilin -
  IL983_RS19650 (IL983_19650) pilB 4275320..4277020 (+) 1701 WP_033956582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IL983_RS19655 (IL983_19655) pilC 4277024..4278241 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  IL983_RS19660 (IL983_19660) pilD 4278242..4279114 (+) 873 WP_034036018.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  IL983_RS19665 (IL983_19665) coaE 4279111..4279722 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  IL983_RS19670 (IL983_19670) yacG 4279719..4279919 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  IL983_RS19675 (IL983_19675) - 4279956..4280165 (-) 210 WP_003094660.1 hypothetical protein -
  IL983_RS19680 (IL983_19680) - 4280271..4280960 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  IL983_RS19685 (IL983_19685) - 4280957..4281427 (-) 471 WP_003094664.1 hypothetical protein -
  IL983_RS19690 (IL983_19690) - 4281424..4281849 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  IL983_RS19695 (IL983_19695) - 4281982..4282611 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  IL983_RS19700 (IL983_19700) - 4282608..4283057 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  IL983_RS19705 (IL983_19705) - 4283083..4283253 (+) 171 WP_003094672.1 DUF3094 family protein -
  IL983_RS19710 (IL983_19710) - 4283317..4284624 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43998.80 Da        Isoelectric Point: 9.8103

>NTDB_id=492525 IL983_RS19655 WP_003161763.1 4277024..4278241(+) (pilC) [Pseudomonas aeruginosa strain KC-Tt-1]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=492525 IL983_RS19655 WP_003161763.1 4277024..4278241(+) (pilC) [Pseudomonas aeruginosa strain KC-Tt-1]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAAAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCAATCGCAGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P3S6C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.275

100

0.617
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

38.155

99.012

0.378