Detailed information NTG0492524

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: IL983_RS19650
• Genome accession: NZ_CP063047
• Coordinates: 4275320..4277020 (+)
• Length: 566 a.a.
• NCBI ID: WP_033956582.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain KC-Tt-1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	4273164..4285722	4275320..4277020	within	0

Gene organization within MGE regions

Location: 4273164..4285722

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IL983_RS19640 (IL983_19640)	-	4273164..4274543 (-)	1380	WP_232095709.1	O-antigen ligase family protein	-
IL983_RS19645 (IL983_19645)	-	4274625..4275089 (-)	465	WP_021205141.1	pilin	-
IL983_RS19650 (IL983_19650)	pilB	4275320..4277020 (+)	1701	WP_033956582.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IL983_RS19655 (IL983_19655)	pilC	4277024..4278241 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
IL983_RS19660 (IL983_19660)	pilD	4278242..4279114 (+)	873	WP_034036018.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
IL983_RS19665 (IL983_19665)	coaE	4279111..4279722 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
IL983_RS19670 (IL983_19670)	yacG	4279719..4279919 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
IL983_RS19675 (IL983_19675)	-	4279956..4280165 (-)	210	WP_003094660.1	hypothetical protein	-
IL983_RS19680 (IL983_19680)	-	4280271..4280960 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
IL983_RS19685 (IL983_19685)	-	4280957..4281427 (-)	471	WP_003094664.1	hypothetical protein	-
IL983_RS19690 (IL983_19690)	-	4281424..4281849 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
IL983_RS19695 (IL983_19695)	-	4281982..4282611 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
IL983_RS19700 (IL983_19700)	-	4282608..4283057 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
IL983_RS19705 (IL983_19705)	-	4283083..4283253 (+)	171	WP_003094672.1	DUF3094 family protein	-
IL983_RS19710 (IL983_19710)	-	4283317..4284624 (+)	1308	WP_003112837.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62313.20 Da
• Isoelectric Point: 5.5798

>NTDB_id=492524 IL983_RS19650 WP_033956582.1 4275320..4277020(+) (pilB) [Pseudomonas aeruginosa strain KC-Tt-1]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=492524 IL983_RS19650 WP_033956582.1 4275320..4277020(+) (pilB) [Pseudomonas aeruginosa strain KC-Tt-1]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCAATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTCTATCTGGCTGCGCTCAAACAGCCACAGGGC
ATGATACTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (190-457)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.544	100	0.58
pilB	Acinetobacter baylyi ADP1	56.79	100	0.569
pilB	Legionella pneumophila strain ERS1305867	54.497	100	0.546
pilB	Vibrio cholerae strain A1552	51.264	97.88	0.502
pilF	Neisseria gonorrhoeae MS11	50.177	99.647	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	52.434	94.346	0.495
pilB	Vibrio campbellii strain DS40M4	51.22	94.17	0.482
pilB/pilB1	Synechocystis sp. PCC 6803	37.763	100	0.412
pilF	Thermus thermophilus HB27	38.664	100	0.389
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.727	97.173	0.376