Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IL983_RS19660 Genome accession   NZ_CP063047
Coordinates   4278242..4279114 (+) Length   290 a.a.
NCBI ID   WP_034036018.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain KC-Tt-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4273164..4285722 4278242..4279114 within 0


Gene organization within MGE regions


Location: 4273164..4285722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL983_RS19640 (IL983_19640) - 4273164..4274543 (-) 1380 WP_232095709.1 O-antigen ligase family protein -
  IL983_RS19645 (IL983_19645) - 4274625..4275089 (-) 465 WP_021205141.1 pilin -
  IL983_RS19650 (IL983_19650) pilB 4275320..4277020 (+) 1701 WP_033956582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IL983_RS19655 (IL983_19655) pilC 4277024..4278241 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  IL983_RS19660 (IL983_19660) pilD 4278242..4279114 (+) 873 WP_034036018.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  IL983_RS19665 (IL983_19665) coaE 4279111..4279722 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  IL983_RS19670 (IL983_19670) yacG 4279719..4279919 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  IL983_RS19675 (IL983_19675) - 4279956..4280165 (-) 210 WP_003094660.1 hypothetical protein -
  IL983_RS19680 (IL983_19680) - 4280271..4280960 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  IL983_RS19685 (IL983_19685) - 4280957..4281427 (-) 471 WP_003094664.1 hypothetical protein -
  IL983_RS19690 (IL983_19690) - 4281424..4281849 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  IL983_RS19695 (IL983_19695) - 4281982..4282611 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  IL983_RS19700 (IL983_19700) - 4282608..4283057 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  IL983_RS19705 (IL983_19705) - 4283083..4283253 (+) 171 WP_003094672.1 DUF3094 family protein -
  IL983_RS19710 (IL983_19710) - 4283317..4284624 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31760.80 Da        Isoelectric Point: 7.3233

>NTDB_id=492526 IL983_RS19660 WP_034036018.1 4278242..4279114(+) (pilD) [Pseudomonas aeruginosa strain KC-Tt-1]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMECNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLGLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=492526 IL983_RS19660 WP_034036018.1 4278242..4279114(+) (pilD) [Pseudomonas aeruginosa strain KC-Tt-1]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGTGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGGGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55

96.552

0.531
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.561

95.862

0.466