Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IHV80_RS13380 Genome accession   NZ_CP062500
Coordinates   3044634..3045059 (+) Length   141 a.a.
NCBI ID   WP_192889368.1    Uniprot ID   -
Organism   Vibrio bathopelagicus strain Sal10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3039634..3050059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV80_RS13365 pdhR 3041561..3042322 (-) 762 WP_004735446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IHV80_RS13370 ampD 3042806..3043404 (-) 599 Protein_2593 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHV80_RS13375 nadC 3043488..3044378 (+) 891 WP_192889367.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IHV80_RS13380 pilA 3044634..3045059 (+) 426 WP_192889368.1 pilin Machinery gene
  IHV80_RS13385 pilB 3045060..3046745 (+) 1686 WP_192889369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHV80_RS13390 pilC 3046760..3047989 (+) 1230 WP_192889370.1 type II secretion system F family protein Machinery gene
  IHV80_RS13395 pilD 3048058..3048927 (+) 870 WP_192889371.1 A24 family peptidase Machinery gene
  IHV80_RS13400 coaE 3048930..3049544 (+) 615 WP_192889372.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14707.87 Da        Isoelectric Point: 7.7745

>NTDB_id=490508 IHV80_RS13380 WP_192889368.1 3044634..3045059(+) (pilA) [Vibrio bathopelagicus strain Sal10]
MNNKNRRTNQKGFTLIELMIVVAIIGALSAIAIPAYKDYVAKSEVAAAVATMKALLTPAELTYQEKGKLAANTDLGDLGI
SAGSNVLGTISVPTDNDIKFLFGSKSAISNAYITYSRTDTGWACAVVSGGVSNLPTIDSCS

Nucleotide


Download         Length: 426 bp        

>NTDB_id=490508 IHV80_RS13380 WP_192889368.1 3044634..3045059(+) (pilA) [Vibrio bathopelagicus strain Sal10]
ATGAATAACAAAAATAGAAGAACAAATCAGAAAGGTTTCACGCTGATTGAATTGATGATTGTGGTGGCAATTATTGGCGC
TCTATCAGCGATCGCGATACCCGCATACAAAGATTACGTAGCAAAAAGTGAAGTTGCAGCAGCAGTAGCAACTATGAAAG
CTTTACTCACGCCGGCAGAACTAACCTATCAAGAAAAAGGGAAATTAGCAGCTAACACTGATTTAGGTGACTTAGGCATC
TCCGCAGGTTCTAATGTATTAGGAACTATTAGTGTACCAACCGACAACGATATTAAATTCCTATTCGGATCAAAAAGTGC
GATATCCAACGCCTACATCACATACTCTAGAACCGACACTGGCTGGGCATGTGCTGTAGTCTCAGGTGGCGTAAGTAACT
TACCAACAATTGATAGTTGCTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(41-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

46.358

100

0.496
  pilA Pseudomonas aeruginosa PAK

43.939

93.617

0.411
  pilA Acinetobacter baumannii strain A118

40.741

95.745

0.39
  pilA Vibrio cholerae C6706

39.416

97.163

0.383
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.416

97.163

0.383
  pilA Vibrio cholerae strain A1552

39.416

97.163

0.383
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.8

88.652

0.362