Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IHV80_RS13385 Genome accession   NZ_CP062500
Coordinates   3045060..3046745 (+) Length   561 a.a.
NCBI ID   WP_192889369.1    Uniprot ID   -
Organism   Vibrio bathopelagicus strain Sal10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3040060..3051745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV80_RS13365 pdhR 3041561..3042322 (-) 762 WP_004735446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IHV80_RS13370 ampD 3042806..3043404 (-) 599 Protein_2593 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHV80_RS13375 nadC 3043488..3044378 (+) 891 WP_192889367.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IHV80_RS13380 pilA 3044634..3045059 (+) 426 WP_192889368.1 pilin Machinery gene
  IHV80_RS13385 pilB 3045060..3046745 (+) 1686 WP_192889369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHV80_RS13390 pilC 3046760..3047989 (+) 1230 WP_192889370.1 type II secretion system F family protein Machinery gene
  IHV80_RS13395 pilD 3048058..3048927 (+) 870 WP_192889371.1 A24 family peptidase Machinery gene
  IHV80_RS13400 coaE 3048930..3049544 (+) 615 WP_192889372.1 dephospho-CoA kinase -
  IHV80_RS13405 zapD 3049571..3050311 (+) 741 WP_192889373.1 cell division protein ZapD -
  IHV80_RS13410 yacG 3050392..3050589 (+) 198 WP_004735515.1 DNA gyrase inhibitor YacG -
  IHV80_RS13415 rplS 3051042..3051395 (-) 354 WP_009847607.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62064.69 Da        Isoelectric Point: 4.9178

>NTDB_id=490509 IHV80_RS13385 WP_192889369.1 3045060..3046745(+) (pilB) [Vibrio bathopelagicus strain Sal10]
MLTNLPTVLRQANLLSLTQEQAVAEHVQASGVSTPEALLLLDIFTGESLAHNIQAIFGLPLVQLTNTDYETLCDQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVLANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMTSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDEIAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVAPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADDSPDIFLRHSIPNSQT
IYKANPQGCNECNQGYSGRVGIYEVMPFSDQLKSSLIDKPNALAIENLAHREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=490509 IHV80_RS13385 WP_192889369.1 3045060..3046745(+) (pilB) [Vibrio bathopelagicus strain Sal10]
GTGCTAACCAACCTCCCAACCGTTCTACGTCAGGCTAATCTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACATGT
GCAAGCTTCAGGTGTTTCTACGCCTGAAGCTTTGCTCCTTCTGGACATATTCACAGGCGAGAGTCTCGCACATAACATTC
AGGCCATCTTCGGCTTACCGTTAGTACAATTAACCAATACTGACTACGAAACCTTGTGTGACCAATTAGGGCTTCGTGAG
CTGATTACCAAGTATCGCGCTATCCCGATTTCAGTTTCAAGTTCAACTCTCACACTTGCCTCGGCCGACCCAACCGACTT
ACAAGCTGAAGATGATTTCCGCTTTGCGACCGGATTACAAATCGAATTGGTTCTCGCTAACTACTCTGAATTAGAAGGCG
CGATACGTAAGCTGTATGGCCGTTCTATTTCCGGGCAAGACTCCAAGCGCAAAGAGATCACTCAAGACGAACTCGCCAAC
CTCGTTGAAGTCTCCGACGATGAGATGACTTCGATTGAAGATCTCAGCCAAGACGACTCCCCTGTTAGCCGCTTTATCAA
CCAAATACTGGTCGATGCGGTACGTAAAGGCGCTTCCGATATCCACTTCGAACCTTATGAAGAAAACTATCGAGTGCGCT
TACGTTGCGATGGCATCCTTGTTGAAATACAACAACCCGCCTCCCATCTAAGTCGCCGTTTATCTGCTCGCTTAAAGATC
CTCGCCAAGCTGGATATCGCTGAGCGTCGCTTGCCTCAAGATGGTCGTATTAAGTTGCGGTTAAACGATGAAATCGCGAT
TGATATGCGAGTATCGACGCTACCGACGCTATGGGGTGAAAAGATCGTACTGCGTCTTCTAGACAGCAGTGCGGCTAATT
TAGATATCGATAAGTTGGGTTATAGCGAGGATCAAAAAGCACTCTACCTCAATGCATTAAAGCGCCCACAAGGTATGATC
TTAATGACCGGGCCAACCGGCAGTGGTAAAACCGTTTCTCTCTACACTGGCCTTCGAGTGCTTAACACCACAGAGCGCAA
CATCTCAACAGCCGAAGACCCGGTAGAGATAAATCTATGTGGTATCAATCAAGTTCAAGTCGCACCCAAAATCGGTTTTG
GGTTTGCCGAAGCATTGCGATCGTTTTTACGACAAGATCCCGATGTGGTCATGGTCGGAGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACTGGACACCTAGTCCTTTCGACATTGCACACCAACTCCGCCGCAGAAACCGT
AACTCGACTCGCTCACATGGGAATTGAACCTTTTAACCTTGCTTCATCATTAAGCTTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCAATCACTGCAAAGCAGCTGACGACTCTCCGGATATATTTCTGCGTCACTCTATCCCTAACAGCCAAACA
ATCTATAAAGCCAATCCACAAGGATGTAATGAGTGTAATCAGGGATACTCTGGGCGAGTGGGTATCTATGAAGTTATGCC
ATTTAGCGACCAACTAAAAAGCAGCCTGATCGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCGCACCGAGAAGGCA
TGCGAACACTGCAAGAATCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAACTACAACGTGTTCTGTAT
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.113

100

0.745
  pilB Vibrio campbellii strain DS40M4

70.463

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

70.463

100

0.706
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Legionella pneumophila strain ERS1305867

49.427

93.405

0.462
  pilB Acinetobacter baylyi ADP1

49.133

92.513

0.455
  pilF Neisseria gonorrhoeae MS11

43.784

98.93

0.433