Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IF189_RS24150 Genome accession   NZ_CP062251
Coordinates   5393199..5394416 (+) Length   405 a.a.
NCBI ID   WP_134828143.1    Uniprot ID   A0A4Y8V8X6
Organism   Pseudomonas sp. IzPS59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5388199..5399416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF189_RS24130 - 5389707..5390003 (+) 297 WP_085712161.1 DUF2845 domain-containing protein -
  IF189_RS24135 - 5390170..5390526 (-) 357 WP_065261581.1 BON domain-containing protein -
  IF189_RS24140 - 5390826..5391269 (-) 444 WP_134828141.1 pilin -
  IF189_RS24145 pilB 5391496..5393196 (+) 1701 WP_192552375.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IF189_RS24150 pilC 5393199..5394416 (+) 1218 WP_134828143.1 type II secretion system F family protein Machinery gene
  IF189_RS24155 pilD 5394418..5395290 (+) 873 WP_134828144.1 prepilin peptidase Machinery gene
  IF189_RS24160 coaE 5395287..5395910 (+) 624 WP_192552376.1 dephospho-CoA kinase -
  IF189_RS24165 yacG 5395907..5396107 (+) 201 WP_085712167.1 DNA gyrase inhibitor YacG -
  IF189_RS24170 - 5396114..5396329 (-) 216 WP_007959584.1 hypothetical protein -
  IF189_RS24175 - 5396395..5397084 (-) 690 WP_134828145.1 energy-coupling factor ABC transporter permease -
  IF189_RS24180 - 5397218..5397844 (+) 627 WP_065261573.1 DUF1780 domain-containing protein -
  IF189_RS24185 - 5397989..5398162 (+) 174 WP_065261571.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44104.95 Da        Isoelectric Point: 10.1093

>NTDB_id=487339 IF189_RS24150 WP_134828143.1 5393199..5394416(+) (pilC) [Pseudomonas sp. IzPS59]
MAVKAAKISVYAWEGTDKKGSRISGELSGQNPALIKAQLRKQGINPGKVRKKSASLLNFGKRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVIGLSEFMQQWWWAILGVLIVAFFGTR
HALKKSQALRDRRDAWLLKLPLVGTMMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVATGM
QLNFAMRTSGIFPNMAVQMTAIGEESGALDDMLDKVAGFYEEEVDNMVDNLTSLMEPFVMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=487339 IF189_RS24150 WP_134828143.1 5393199..5394416(+) (pilC) [Pseudomonas sp. IzPS59]
ATGGCCGTCAAGGCAGCGAAAATCAGCGTCTACGCCTGGGAAGGCACGGACAAGAAAGGCAGCCGGATCAGCGGCGAACT
GAGCGGGCAAAACCCCGCGCTGATCAAGGCCCAGTTGCGCAAGCAGGGCATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CGTCGTTGCTGAATTTCGGCAAACGCATCAAGCCGCTGGATATCGCCCTGTTCACCCGGCAGATGGCAACCATGATGAAG
GCCGGCGTGCCGTTATTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGAGAACCCTGCGATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGTAACAGCTTCGCCGCGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGTGCCCTCGACACCCTGCTCGAGCGCGTGGCGACCTACAAAGAGAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCCACCGCCGTGATTCTGGTGGCGGCGGTCGTCACCGGCAT
TCTGCTGGTGAAAGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGCTTCGGTGCCGAACTGCCGGCGTTTACCTTGATGG
TGATCGGCCTGTCGGAGTTCATGCAGCAATGGTGGTGGGCGATTCTGGGTGTGCTGATTGTGGCGTTTTTCGGCACCCGC
CATGCCTTGAAGAAATCCCAGGCCCTGCGTGACCGCCGAGATGCCTGGCTACTGAAACTGCCACTGGTGGGCACCATGAT
GTACAAGTCCGCCGTGGCCCGTTTTGCCCGGACCCTGTCGACCACGTTTGCCGCCGGCGTGCCGCTGGTCGAAGCGCTGG
ACTCGGTCGCCGGAGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCTGCGCATTCGTCAGGACGTGGCCACCGGCATG
CAGTTGAATTTCGCGATGCGCACCAGCGGAATCTTTCCGAACATGGCCGTGCAGATGACCGCCATCGGCGAAGAATCCGG
CGCCCTGGACGACATGCTCGACAAGGTGGCCGGGTTCTACGAGGAAGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCTTTCGTCATGGTGGTACTCGGGGTGATCGTCGGCGGGCTGGTCGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y8V8X6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baumannii D1279779

59.314

100

0.598
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.75

98.765

0.531
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilC Vibrio campbellii strain DS40M4

41.278

100

0.415
  pilC Thermus thermophilus HB27

38.213

99.506

0.38