Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IF189_RS24145 Genome accession   NZ_CP062251
Coordinates   5391496..5393196 (+) Length   566 a.a.
NCBI ID   WP_192552375.1    Uniprot ID   -
Organism   Pseudomonas sp. IzPS59     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5386496..5398196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF189_RS24125 - 5386756..5389707 (+) 2952 WP_192552374.1 DUF748 domain-containing protein -
  IF189_RS24130 - 5389707..5390003 (+) 297 WP_085712161.1 DUF2845 domain-containing protein -
  IF189_RS24135 - 5390170..5390526 (-) 357 WP_065261581.1 BON domain-containing protein -
  IF189_RS24140 - 5390826..5391269 (-) 444 WP_134828141.1 pilin -
  IF189_RS24145 pilB 5391496..5393196 (+) 1701 WP_192552375.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IF189_RS24150 pilC 5393199..5394416 (+) 1218 WP_134828143.1 type II secretion system F family protein Machinery gene
  IF189_RS24155 pilD 5394418..5395290 (+) 873 WP_134828144.1 prepilin peptidase Machinery gene
  IF189_RS24160 coaE 5395287..5395910 (+) 624 WP_192552376.1 dephospho-CoA kinase -
  IF189_RS24165 yacG 5395907..5396107 (+) 201 WP_085712167.1 DNA gyrase inhibitor YacG -
  IF189_RS24170 - 5396114..5396329 (-) 216 WP_007959584.1 hypothetical protein -
  IF189_RS24175 - 5396395..5397084 (-) 690 WP_134828145.1 energy-coupling factor ABC transporter permease -
  IF189_RS24180 - 5397218..5397844 (+) 627 WP_065261573.1 DUF1780 domain-containing protein -
  IF189_RS24185 - 5397989..5398162 (+) 174 WP_065261571.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62564.54 Da        Isoelectric Point: 5.6826

>NTDB_id=487338 IF189_RS24145 WP_192552375.1 5391496..5393196(+) (pilB) [Pseudomonas sp. IzPS59]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLTTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESVDDSKQDAIGGMDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGGFTIYEPVGCDHCNGGYKGRTGIYEVVRNTPELQRLIMAEGNSLEIDSQMRLDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=487338 IF189_RS24145 WP_192552375.1 5391496..5393196(+) (pilB) [Pseudomonas sp. IzPS59]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAAAAAGCGCCCAACAAGC
CTGGCAGCAAGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTACAGAACAAACTGGTGAAGAGTTCGCAGGTTG
CCGAAATCGCCTCGGAGCACTTCGGCATGGCGTTCCTCGACCTCAACTGCCTGGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTCCGCCAGCACCATGCCCTGCCCCTGTGGCGACGCGGCAACAAACTGTTCGTAGGGATCTCCGA
CCCAAGCAATCACCAGGCGATCAACGACATTCAGTTCAGTACCGGGCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCACCGACGCCATCGAGAAATTCTTCGACACCCACGCCTCCGGCCTGGAAGACATGGCCGATGTCGACCTCGACGGG
CTGGACGTCGAATCCGTCGATGACAGCAAACAGGACGCCATCGGTGGAATGGACGCCGACGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGCTGCTGGACGCAATCAAGAGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAAGTGGCCAAACCGCCGATTCAACTGGCCGGACGCATCGCCGCCCGGCTG
AAGGTCATGGCCAGCCTCGACATCTCGGAACGCCGAAAACCCCAGGACGGACGCCTGAAGATGCGCTTGTCGAAAACCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAGGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CACAGATCGGCATCGACGCCCTCGGCTACGAGCCGGAGCAGAAAGAGCTGTACCTGGCCGCCCTGAAGCAGCCGCAAGGC
ATGATCCTGGTCACCGGCCCCACCGGTTCCGGCAAGACCGTGTCGCTGTACACCGGGCTCAACATCCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAGCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTC
GAAACAGCGGAGATTGCGATCAAGGCGGCACAGACCGGTCACCTTGTACTGTCCACGCTGCACACCAACAGCGCCGCAGA
AACGCTGACCCGCTTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACGTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAACTCTGCAGCCACTGCAAGAAGCCCATCGAGATTCCCCGCGAAACCCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCGGTTTCACGATCTATGAGCCCGTCGGTTGCGATCACTGCAACGGCGGCTACAAGGGCCGCACCGGCAT
CTATGAAGTGGTCAGGAACACGCCCGAACTGCAGCGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGACAGCCAGA
TGCGCCTGGACGGCTTCAACGACCTGCGCACCTCGGGACTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

99.823

0.58
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.267

99.117

0.498
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilF Thermus thermophilus HB27

36.918

98.587

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

93.64

0.362