Detailed information NTG0487340

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IF189_RS24155
• Genome accession: NZ_CP062251
• Coordinates: 5394418..5395290 (+)
• Length: 290 a.a.
• NCBI ID: WP_134828144.1
• Uniprot ID: A0A4Y8VC64
• Organism: Pseudomonas sp. IzPS59
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5389418..5400290

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IF189_RS24130	-	5389707..5390003 (+)	297	WP_085712161.1	DUF2845 domain-containing protein	-
IF189_RS24135	-	5390170..5390526 (-)	357	WP_065261581.1	BON domain-containing protein	-
IF189_RS24140	-	5390826..5391269 (-)	444	WP_134828141.1	pilin	-
IF189_RS24145	pilB	5391496..5393196 (+)	1701	WP_192552375.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IF189_RS24150	pilC	5393199..5394416 (+)	1218	WP_134828143.1	type II secretion system F family protein	Machinery gene
IF189_RS24155	pilD	5394418..5395290 (+)	873	WP_134828144.1	prepilin peptidase	Machinery gene
IF189_RS24160	coaE	5395287..5395910 (+)	624	WP_192552376.1	dephospho-CoA kinase	-
IF189_RS24165	yacG	5395907..5396107 (+)	201	WP_085712167.1	DNA gyrase inhibitor YacG	-
IF189_RS24170	-	5396114..5396329 (-)	216	WP_007959584.1	hypothetical protein	-
IF189_RS24175	-	5396395..5397084 (-)	690	WP_134828145.1	energy-coupling factor ABC transporter permease	-
IF189_RS24180	-	5397218..5397844 (+)	627	WP_065261573.1	DUF1780 domain-containing protein	-
IF189_RS24185	-	5397989..5398162 (+)	174	WP_065261571.1	DUF3094 domain-containing protein	-
IF189_RS24190	-	5398229..5399527 (+)	1299	WP_085712169.1	NAD(P)/FAD-dependent oxidoreductase	-
IF189_RS24195	-	5399739..5400146 (+)	408	WP_114885602.1	EF-hand domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32406.78 Da
• Isoelectric Point: 7.7030

>NTDB_id=487340 IF189_RS24155 WP_134828144.1 5394418..5395290(+) (pilD) [Pseudomonas sp. IzPS59]
MPIDELFALYPLAFVFTALVLGLVVGSFLNVVIWRLPKMLEREWRQQAHDVLGLPVETPLPVYNLMLPHSQCPHCAHRIR
AWENIPLLSYLFLRGRCSSCAAPISKRYPLTELACGLLSAFIAWHLGFGWPACLLIVLTWGLLAMSLIDTEHQLLPDVLV
LPLLWLGLIVNSFDLFVSLHDALWGTVAGYMALWSVFWLFKLLTGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVG
AVLGVILLKLRDRKTSTPIPFGPYLAIAGWIALLWGGQITDFYWQFVGLK

Nucleotide

• Length: 873 bp

>NTDB_id=487340 IF189_RS24155 WP_134828144.1 5394418..5395290(+) (pilD) [Pseudomonas sp. IzPS59]
ATGCCCATCGACGAACTCTTCGCCCTCTATCCGCTGGCCTTTGTCTTCACTGCCCTAGTGCTCGGGCTGGTGGTCGGCAG
CTTCCTCAATGTGGTGATCTGGCGTCTGCCGAAGATGCTCGAGCGCGAATGGCGCCAGCAGGCCCACGACGTGCTCGGGC
TGCCCGTCGAAACGCCGTTGCCGGTCTACAACCTGATGTTGCCGCACTCGCAATGCCCGCATTGCGCTCACCGCATTCGT
GCGTGGGAAAACATCCCGCTGCTCAGCTACCTGTTTTTGCGCGGGCGCTGCTCGTCATGCGCGGCGCCGATCAGCAAGCG
TTATCCTCTGACCGAACTGGCCTGCGGCCTGCTCTCGGCGTTCATCGCCTGGCATCTGGGCTTCGGCTGGCCGGCATGCC
TGCTGATCGTGCTGACCTGGGGCTTGCTGGCGATGAGCCTGATCGACACCGAGCATCAGCTGCTGCCCGACGTGCTGGTA
CTGCCGCTGCTGTGGCTGGGCTTGATCGTCAACAGCTTCGACCTGTTCGTCTCATTGCATGACGCGCTGTGGGGCACGGT
GGCCGGTTACATGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGCTCACCGGCAAGGACGGCATCGGTCACGGCGACT
TCAAGCTGCTGGCACTGCTGGGGGCCTGGGGAGGCTGGCAGATTCTGCCGCTGACCATTCTCCTGTCGTCGCTGGTGGGT
GCCGTACTGGGAGTGATTCTGCTCAAGCTGCGCGACCGGAAAACCTCGACGCCAATCCCCTTCGGGCCCTATCTGGCAAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: A0A4Y8VC64

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.901	97.241	0.524
pilD	Vibrio campbellii strain DS40M4	51.418	97.241	0.5
pilD	Acinetobacter baumannii D1279779	48.944	97.931	0.479
pilD	Acinetobacter nosocomialis M2	48.944	97.931	0.479
pilD	Neisseria gonorrhoeae MS11	48.148	93.103	0.448