Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IF561_RS00195 Genome accession   NZ_CP062150
Coordinates   30739..31197 (-) Length   152 a.a.
NCBI ID   WP_025536236.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 19-VB00998     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 25739..36197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF561_RS00175 (IF561_00175) coaE 26258..26872 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  IF561_RS00180 (IF561_00180) pilD 26873..27742 (-) 870 WP_025533026.1 A24 family peptidase Machinery gene
  IF561_RS00185 (IF561_00185) pilC 27807..29030 (-) 1224 WP_029804706.1 type II secretion system F family protein Machinery gene
  IF561_RS00190 (IF561_00190) pilB 29054..30739 (-) 1686 WP_133280437.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IF561_RS00195 (IF561_00195) pilA 30739..31197 (-) 459 WP_025536236.1 pilin Machinery gene
  IF561_RS00200 (IF561_00200) nadC 31460..32347 (-) 888 WP_005479699.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IF561_RS00205 (IF561_00205) ampD 32440..32991 (+) 552 WP_005479703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IF561_RS00210 (IF561_00210) pdhR 33397..34164 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15647.94 Da        Isoelectric Point: 8.4867

>NTDB_id=486441 IF561_RS00195 WP_025536236.1 30739..31197(-) (pilA) [Vibrio parahaemolyticus strain 19-VB00998]
MKHSKQKKQQGFTLIELMIVVAVIGVLAAIAMPQYQKYVAKSEVASVLATLTGAKTNVEAYTVENGLFPDGSASDATPTA
LGVPSMHLGTVAFTDQSADGGKISFTFATTASAGVSALVSGKKLTLTRTATSGGWDCSSSDLGSELLPKTCK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=486441 IF561_RS00195 WP_025536236.1 30739..31197(-) (pilA) [Vibrio parahaemolyticus strain 19-VB00998]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACGCTAATTGAATTGATGATTGTGGTGGCGGTAATTGGTGTTTT
GGCGGCGATTGCAATGCCACAATACCAAAAGTATGTAGCTAAAAGTGAAGTTGCATCTGTTTTAGCAACATTGACAGGGG
CTAAAACTAATGTAGAAGCTTACACTGTAGAGAACGGTTTATTTCCAGATGGCTCAGCTTCTGATGCAACACCAACGGCC
CTAGGGGTTCCATCAATGCATTTAGGAACTGTAGCTTTTACAGACCAAAGTGCTGATGGTGGCAAAATTTCTTTTACATT
TGCAACAACAGCTAGTGCGGGTGTTAGTGCTTTAGTTTCAGGGAAAAAACTGACTCTTACGCGAACGGCAACATCTGGAG
GATGGGATTGCTCATCATCTGATTTGGGTAGCGAATTGCTTCCTAAAACTTGTAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.968

100

0.52
  pilA Vibrio cholerae strain A1552

50.968

100

0.52
  pilA Vibrio cholerae C6706

50.968

100

0.52
  pilA Vibrio parahaemolyticus RIMD 2210633

49.64

91.447

0.454
  pilA Pseudomonas aeruginosa PAK

45.033

99.342

0.447
  pilA Acinetobacter baumannii strain A118

43.243

97.368

0.421
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.259

94.079

0.388
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.776

96.711

0.375
  pilA/pilA1 Eikenella corrodens VA1

34.591

100

0.362