Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IG194_RS19895 Genome accession   NZ_CP062122
Coordinates   4368254..4369471 (-) Length   405 a.a.
NCBI ID   WP_058488548.1    Uniprot ID   A0A1A5D7D3
Organism   Pseudomonas sp. ADPe     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4363254..4374471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG194_RS19850 (IG194_19860) - 4363820..4364236 (+) 417 WP_058488542.1 GNAT family N-acetyltransferase -
  IG194_RS19855 (IG194_19865) - 4364239..4364715 (+) 477 WP_058488543.1 hypothetical protein -
  IG194_RS19860 (IG194_19870) - 4364712..4365428 (+) 717 WP_058488544.1 energy-coupling factor ABC transporter permease -
  IG194_RS19865 (IG194_19875) - 4365536..4365751 (+) 216 WP_058488545.1 hypothetical protein -
  IG194_RS19870 (IG194_19880) - 4365758..4366033 (-) 276 WP_024128153.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
  IG194_RS19875 (IG194_19885) - 4366033..4366227 (-) 195 WP_009617620.1 hypothetical protein -
  IG194_RS19880 (IG194_19890) yacG 4366493..4366702 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  IG194_RS19885 (IG194_19895) coaE 4366699..4367307 (-) 609 WP_058488546.1 dephospho-CoA kinase -
  IG194_RS19890 (IG194_19900) pilD 4367381..4368253 (-) 873 WP_058488547.1 prepilin peptidase Machinery gene
  IG194_RS19895 (IG194_19905) pilC 4368254..4369471 (-) 1218 WP_058488548.1 type II secretion system F family protein Machinery gene
  IG194_RS19900 (IG194_19910) pilB 4369475..4371178 (-) 1704 WP_058489715.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IG194_RS19905 (IG194_19915) - 4371411..4371887 (+) 477 WP_082996272.1 pilin -
  IG194_RS19915 (IG194_19925) - 4372267..4373340 (+) 1074 WP_058488549.1 diguanylate cyclase -
  IG194_RS33350 - 4373337..4373543 (-) 207 Protein_3950 type II toxin-antitoxin system HipA family toxin -
  IG194_RS19920 (IG194_19930) - 4373586..4373837 (+) 252 WP_058488551.1 type II toxin-antitoxin system HicA family toxin -
  IG194_RS19925 (IG194_19935) - 4373834..4374175 (+) 342 WP_043318088.1 type II toxin-antitoxin system HicB family antitoxin -
  IG194_RS19930 (IG194_19940) - 4374241..4374468 (+) 228 WP_043272290.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44106.77 Da        Isoelectric Point: 9.8268

>NTDB_id=486173 IG194_RS19895 WP_058488548.1 4368254..4369471(-) (pilC) [Pseudomonas sp. ADPe]
MAEKALKTSVFAFEGTDRKGGKIKGELSGTNVALVKAQLRKQGINPIKVRKKGVSLLSKGKKIKPMDIALFTRQMATMMG
SGVPLLQSFDIIAEGFDNPNMRKLVDDIKQEVAAGNSLANSLRKKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVAIIVSAILLIKVVPQFQSVFANFGAELPAFTLMVIGLSEVLQEWWFIVLIALFALAFAFR
EAHRRSERFRDSIDRATLKLPIVGGIIYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVTKIKQDVSSGT
QLNFSMRTTGVFPTMAVQMAAIGEESGSLDSMLDKVANYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=486173 IG194_RS19895 WP_058488548.1 4368254..4369471(-) (pilC) [Pseudomonas sp. ADPe]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTCTTCGCATTTGAAGGCACGGATCGCAAGGGCGGCAAGATCAAGGGAGAGCT
ATCTGGCACGAATGTCGCTCTGGTCAAGGCGCAATTGCGCAAACAGGGCATCAACCCGATCAAGGTTCGCAAGAAGGGCG
TCTCGCTGCTGAGCAAGGGCAAGAAGATCAAGCCTATGGACATCGCCCTTTTCACTCGGCAGATGGCGACCATGATGGGC
TCAGGCGTACCCTTGCTGCAGTCCTTCGACATCATCGCGGAGGGTTTCGACAACCCCAACATGAGGAAGCTGGTCGACGA
CATCAAACAGGAAGTGGCTGCGGGCAACAGCTTAGCCAACTCGTTGCGCAAGAAACCACAGTACTTCGATGACCTCTACT
GCAACCTAGTCGACGCAGGTGAACAATCCGGTGCACTGGAAACCCTGTTGGATCGCGTAGCCACCTACAAAGAAAAGACC
GAGTCACTGAAAGCCAAGATCAAGAAGGCGATGACCTATCCGATAGCGGTAATCATCGTTGCAATTATCGTATCTGCAAT
CCTTCTCATCAAAGTGGTCCCCCAATTCCAGTCCGTATTCGCCAACTTCGGCGCCGAACTTCCTGCCTTCACTCTGATGG
TTATCGGTCTCTCAGAAGTATTACAGGAATGGTGGTTTATCGTCTTGATCGCCTTGTTCGCGCTGGCTTTCGCCTTCCGT
GAGGCGCACCGCCGATCAGAGAGATTCCGCGACTCAATAGACAGGGCAACCCTTAAGCTCCCCATCGTTGGCGGCATTAT
CTACAAATCTGCTGTCGCCCGTTATGCACGCACCCTGTCCACCACGTTCGCGGCAGGCGTTCCCTTGGTGGAAGCACTGG
ACTCGGTATCCGGCGCCACTGGCAACGTCGTGTTCCGCAACGCAGTGACCAAGATCAAGCAAGACGTATCCTCGGGCACC
CAGCTCAACTTCTCCATGCGCACCACCGGTGTCTTTCCCACCATGGCCGTACAAATGGCGGCCATTGGCGAGGAGTCCGG
CTCTCTGGACTCCATGTTGGACAAGGTCGCCAACTACTACGAAGAGGAAGTGGACAACGCTGTCGATAACCTCACCACCC
TGATGGAACCCATGATCATGGCCGTACTTGGTGTACTAGTCGGCGGCTTGATCATCGCCATGTACCTCCCTATCTTCCAA
CTCGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A5D7D3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.741

100

0.807
  pilC Acinetobacter baumannii D1279779

61.481

100

0.615
  pilC Acinetobacter baylyi ADP1

60.591

100

0.607
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilG Neisseria gonorrhoeae MS11

45.274

99.259

0.449
  pilC Vibrio cholerae strain A1552

40.494

100

0.405
  pilC Vibrio campbellii strain DS40M4

39.899

97.778

0.39
  pilC Thermus thermophilus HB27

37.656

99.012

0.373