Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IG194_RS19890 Genome accession   NZ_CP062122
Coordinates   4367381..4368253 (-) Length   290 a.a.
NCBI ID   WP_058488547.1    Uniprot ID   A0A1A5D834
Organism   Pseudomonas sp. ADPe     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4362381..4373253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG194_RS19840 (IG194_19850) - 4362815..4362988 (-) 174 WP_009617611.1 DUF3094 family protein -
  IG194_RS19845 (IG194_19855) - 4363058..4363687 (-) 630 WP_058488541.1 DUF1780 domain-containing protein -
  IG194_RS19850 (IG194_19860) - 4363820..4364236 (+) 417 WP_058488542.1 GNAT family N-acetyltransferase -
  IG194_RS19855 (IG194_19865) - 4364239..4364715 (+) 477 WP_058488543.1 hypothetical protein -
  IG194_RS19860 (IG194_19870) - 4364712..4365428 (+) 717 WP_058488544.1 energy-coupling factor ABC transporter permease -
  IG194_RS19865 (IG194_19875) - 4365536..4365751 (+) 216 WP_058488545.1 hypothetical protein -
  IG194_RS19870 (IG194_19880) - 4365758..4366033 (-) 276 WP_024128153.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
  IG194_RS19875 (IG194_19885) - 4366033..4366227 (-) 195 WP_009617620.1 hypothetical protein -
  IG194_RS19880 (IG194_19890) yacG 4366493..4366702 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  IG194_RS19885 (IG194_19895) coaE 4366699..4367307 (-) 609 WP_058488546.1 dephospho-CoA kinase -
  IG194_RS19890 (IG194_19900) pilD 4367381..4368253 (-) 873 WP_058488547.1 prepilin peptidase Machinery gene
  IG194_RS19895 (IG194_19905) pilC 4368254..4369471 (-) 1218 WP_058488548.1 type II secretion system F family protein Machinery gene
  IG194_RS19900 (IG194_19910) pilB 4369475..4371178 (-) 1704 WP_058489715.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IG194_RS19905 (IG194_19915) - 4371411..4371887 (+) 477 WP_082996272.1 pilin -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31997.98 Da        Isoelectric Point: 7.0170

>NTDB_id=486172 IG194_RS19890 WP_058488547.1 4367381..4368253(-) (pilD) [Pseudomonas sp. ADPe]
MLVFDFLASHALAFVLCAALLGLLVGSFLNVVIHRLPKMMELEWQQQARDALSLPQPEQKATYNLVLPNSACPHCGHEIR
AWENIPVISYLALRGKCSACKVPISARYPLVELATALLSGFVAWHFGFTWQAGAMLLLTWGLLAMSLIDVDHQLLPDALV
LPLLWLGLIVNYFGLFTSLGDALWGAVFGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILFSSLVG
AVLGVILLRLRNAETSTPIPFGPYLAIAGWIALLWGDQITTTYLQFAGFR

Nucleotide


Download         Length: 873 bp        

>NTDB_id=486172 IG194_RS19890 WP_058488547.1 4367381..4368253(-) (pilD) [Pseudomonas sp. ADPe]
ATGCTCGTATTCGACTTCCTGGCCAGCCACGCGCTGGCCTTTGTTTTATGCGCCGCCCTGCTCGGCCTTCTGGTCGGCAG
CTTCCTCAACGTGGTCATCCATCGCCTGCCGAAGATGATGGAACTGGAGTGGCAGCAACAAGCGCGTGACGCTCTTTCCC
TGCCTCAGCCTGAGCAGAAAGCCACCTACAACCTGGTCCTACCCAACTCGGCGTGCCCGCATTGCGGTCATGAAATCCGT
GCCTGGGAGAACATCCCCGTCATCAGCTACCTGGCCCTGCGCGGCAAGTGCTCGGCCTGCAAGGTGCCGATCAGTGCGCG
TTATCCGCTGGTCGAGCTGGCCACCGCCCTGCTTTCCGGTTTCGTTGCCTGGCACTTCGGCTTTACCTGGCAGGCCGGGG
CCATGCTGTTGCTGACCTGGGGGCTGTTGGCCATGAGCCTGATCGATGTCGATCACCAGTTGCTGCCGGATGCGCTGGTG
CTGCCGCTGCTCTGGCTGGGCTTGATCGTCAATTACTTCGGGCTTTTCACCAGCCTGGGCGATGCGCTCTGGGGTGCGGT
GTTCGGTTACCTGAGCCTGTGGTCGGTGTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGCTGCTGGCCATGCTCGGCGCCTGGGGTGGGTGGCAGGTGTTGCCGCTGACCATTCTGTTCTCGTCCCTGGTCGGG
GCCGTGCTGGGGGTCATTCTGCTGCGTCTGCGCAATGCCGAGACCAGCACGCCGATCCCCTTCGGGCCCTATCTGGCCAT
TGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACCACTACCTATCTGCAGTTCGCCGGTTTCCGGTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A5D834

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

58.696

95.172

0.559
  pilD Vibrio campbellii strain DS40M4

56.777

94.138

0.534
  pilD Acinetobacter baumannii D1279779

53.684

98.276

0.528
  pilD Acinetobacter nosocomialis M2

53.333

98.276

0.524
  pilD Neisseria gonorrhoeae MS11

50.57

90.69

0.459