Detailed information NTG0486174

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: IG194_RS19900
• Genome accession: NZ_CP062122
• Coordinates: 4369475..4371178 (-)
• Length: 567 a.a.
• NCBI ID: WP_058489715.1
• Uniprot ID: A0A1A5D854
• Organism: Pseudomonas sp. ADPe
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4364475..4376178

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IG194_RS19860 (IG194_19870)	-	4364712..4365428 (+)	717	WP_058488544.1	energy-coupling factor ABC transporter permease	-
IG194_RS19865 (IG194_19875)	-	4365536..4365751 (+)	216	WP_058488545.1	hypothetical protein	-
IG194_RS19870 (IG194_19880)	-	4365758..4366033 (-)	276	WP_024128153.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
IG194_RS19875 (IG194_19885)	-	4366033..4366227 (-)	195	WP_009617620.1	hypothetical protein	-
IG194_RS19880 (IG194_19890)	yacG	4366493..4366702 (-)	210	WP_043272268.1	DNA gyrase inhibitor YacG	-
IG194_RS19885 (IG194_19895)	coaE	4366699..4367307 (-)	609	WP_058488546.1	dephospho-CoA kinase	-
IG194_RS19890 (IG194_19900)	pilD	4367381..4368253 (-)	873	WP_058488547.1	prepilin peptidase	Machinery gene
IG194_RS19895 (IG194_19905)	pilC	4368254..4369471 (-)	1218	WP_058488548.1	type II secretion system F family protein	Machinery gene
IG194_RS19900 (IG194_19910)	pilB	4369475..4371178 (-)	1704	WP_058489715.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IG194_RS19905 (IG194_19915)	-	4371411..4371887 (+)	477	WP_082996272.1	pilin	-
IG194_RS19915 (IG194_19925)	-	4372267..4373340 (+)	1074	WP_058488549.1	diguanylate cyclase	-
IG194_RS33350	-	4373337..4373543 (-)	207	Protein_3950	type II toxin-antitoxin system HipA family toxin	-
IG194_RS19920 (IG194_19930)	-	4373586..4373837 (+)	252	WP_058488551.1	type II toxin-antitoxin system HicA family toxin	-
IG194_RS19925 (IG194_19935)	-	4373834..4374175 (+)	342	WP_043318088.1	type II toxin-antitoxin system HicB family antitoxin	-
IG194_RS19930 (IG194_19940)	-	4374241..4374468 (+)	228	WP_043272290.1	DUF433 domain-containing protein	-
IG194_RS19935 (IG194_19945)	-	4374656..4375852 (-)	1197	WP_058488552.1	type II toxin-antitoxin system HipA family toxin	-
IG194_RS19940 (IG194_19950)	-	4375852..4376103 (-)	252	WP_009617650.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62598.63 Da
• Isoelectric Point: 5.4516

>NTDB_id=486174 IG194_RS19900 WP_058489715.1 4369475..4371178(-) (pilB) [Pseudomonas sp. ADPe]
MNDQVMLSGLARQLVVAELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAAEQFGIAFCDLGAIDKDSLPRD
LISEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGDTIEKLFESATGGLDDLADVDLD
GLDIESGDADRKEESIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDVPNETLLHEGFP
EDRIGSFKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLVKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=486174 IG194_RS19900 WP_058489715.1 4369475..4371178(-) (pilB) [Pseudomonas sp. ADPe]
ATGAACGACCAAGTCATGCTTTCCGGGCTTGCCCGCCAACTGGTGGTAGCTGAGTTACTCGACGAAAAAAGCGCTCAACA
AGCACAGCAGCAAGCACAGCGGAACAAGCTCTCGCTGGTGACTTACCTCGTACAGAACAAACTGGTGAAGAGTCGGCCAC
TTACCGAGTTGGCCGCTGAGCAGTTCGGAATAGCCTTCTGTGATCTGGGCGCCATAGACAAGGACAGCCTTCCAAGGGAT
CTGATCAGCGAAAAGCTGGTACGCCAACACCGCGTCATCCCACTCTGGCGGCGCGGAAATAAGCTATTTGTGGGCATTTC
CGACCCAACCAATCACCAAGCCATTACTGACGTCCAGTTCAGCACCGGCCTGACCACTGAGGCGATTCTGGTCGAAGACG
ACAAGCTCGGTGACACCATTGAGAAGCTCTTCGAAAGTGCTACCGGCGGCCTGGACGATCTGGCCGACGTCGATTTGGAT
GGGCTGGACATTGAAAGTGGTGATGCAGACCGCAAGGAAGAAAGTATCGGAGGAGATGCTGACGATGCTCCAGTAGTCCG
CTTCGTCAACAAGATGCTACTGGACGCCATCAAGGGAGGCTCATCGGACCTACACTTCGAGCCCTACGAGAAGATCTACC
GCGTACGCTTCCGTACCGATGGCATGCTGCATGAGGTGGCCAAGCCTCCAATCCAACTGGCTAGCCGTATCTCGGCGCGC
CTGAAAGTAATGGCCGGACTGGATATTTCCGAAAGGCGCAAGCCTCAGGACGGCCGCATCAAGATGCGTGTATCCAAGAG
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCTACCCTGTGGGGTGAGAAGATCGTGATGCGTATTCTCGACTCGGCCA
GCGCACAGATGGGCATTGATGCCCTTGGCTATGAGGATGCGCAGAAACAGCTCTATCTTGAAGCCTTGAAGCAACCACAG
GGCATGATCCTGGTGACCGGACCAACCGGCTCCGGCAAGACCGTCTCCCTGTATACCGGCTTGAATATCCTCAATACTCC
GGACATCAACATCTCCACCGCTGAAGATCCAGTGGAGATCAACCTGGAGGGCATCAATCAAGTCAATGTCAATCCACGCC
AGGGCATGGACTTCGCTCAGGCGTTGCGCGCCTTCCTCCGTCAGGATCCAGACGTGATCATGGTTGGTGAGATCCGCGAC
CTTGAAACCGCAGAGATCGCCATCAAGGCCGCACAGACTGGTCACATGGTGATGTCCACCTTGCACACCAACAGTGCTGC
GGAAACCCTGACGCGCCTGCTGAACATGGGAGTAGCATCCTTCAACCTGGCTACTTCCACCAATCTGATCATCGCCCAGC
GCCTGGCACGCAAGCTGTGCCCGCACTGCAAGAAGGAACACGACGTCCCCAACGAAACCCTGCTCCATGAAGGTTTTCCC
GAGGACAGGATCGGCAGCTTCAAGCTGTACGCCCCCGTGGGGTGTGAAAATTGCAAGAACGGCTACAAGGGCCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAAGAAGGCTTCAATGACCTGCGTACCTCGGGCCTGGTCAAGGCCATGCAAGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

3D structure

• AlphaFold DB: A0A1A5D854

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	58.259	99.295	0.578
pilB	Acinetobacter baumannii D1279779	57.699	99.647	0.575
pilB	Legionella pneumophila strain ERS1305867	54.482	100	0.547
pilB	Vibrio cholerae strain A1552	50.98	98.942	0.504
pilB	Vibrio campbellii strain DS40M4	50	99.824	0.499
pilB	Vibrio parahaemolyticus RIMD 2210633	51.934	95.767	0.497
pilF	Neisseria gonorrhoeae MS11	49.647	99.824	0.496
pilF	Thermus thermophilus HB27	38.977	100	0.39
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.698	93.298	0.37