Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   KBP49_RS09795 Genome accession   NZ_CP072933
Coordinates   2220927..2221373 (+) Length   148 a.a.
NCBI ID   WP_071869805.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain AlmaEM3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2215927..2226373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBP49_RS09775 (KBP49_09775) - 2216175..2217512 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  KBP49_RS09780 (KBP49_09780) coaE 2217779..2218402 (-) 624 WP_027700146.1 dephospho-CoA kinase -
  KBP49_RS09785 (KBP49_09785) pilD 2218414..2219277 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  KBP49_RS09790 (KBP49_09790) pilC 2219284..2220486 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  KBP49_RS09795 (KBP49_09795) pilA 2220927..2221373 (+) 447 WP_071869805.1 pilin Machinery gene
  KBP49_RS09800 (KBP49_09800) - 2221618..2221845 (-) 228 WP_012338102.1 hypothetical protein -
  KBP49_RS09805 (KBP49_09805) pilA/pilAI 2222678..2223124 (+) 447 WP_071869804.1 pilin Machinery gene
  KBP49_RS09810 (KBP49_09810) pilB 2223964..2225697 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15372.58 Da        Isoelectric Point: 8.4988

>NTDB_id=485898 KBP49_RS09795 WP_071869805.1 2220927..2221373(+) (pilA) [Xylella fastidiosa subsp. multiplex strain AlmaEM3]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKATSNASDIGLRTDTTRCGI
TVKVEAAGTANITCKVKGNSQVSDKTIAWDRTPDNSAGTNGVNNGGVWTCSTTVTSDALRPSGCIATK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=485898 KBP49_RS09795 WP_071869805.1 2220927..2221373(+) (pilA) [Xylella fastidiosa subsp. multiplex strain AlmaEM3]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGGCTACATCCAATGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTAAAGTCGAGGCCGCTGGCACCGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAGTGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCAACCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATTGCCACTAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40

100

0.419

  pilA Vibrio cholerae strain A1552

40

100

0.419

  pilA Vibrio cholerae C6706

40

100

0.419

  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.497

99.324

0.412

  pilA Pseudomonas aeruginosa PAK

37.736

100

0.405

  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.816

99.324

0.405

  pilA Acinetobacter baumannii strain A118

38

100

0.385

  pilA Vibrio parahaemolyticus RIMD 2210633

36.486

100

0.365