Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   KBP49_RS09805 Genome accession   NZ_CP072933
Coordinates   2222678..2223124 (+) Length   148 a.a.
NCBI ID   WP_071869804.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain AlmaEM3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2217678..2228124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBP49_RS09780 (KBP49_09780) coaE 2217779..2218402 (-) 624 WP_027700146.1 dephospho-CoA kinase -
  KBP49_RS09785 (KBP49_09785) pilD 2218414..2219277 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  KBP49_RS09790 (KBP49_09790) pilC 2219284..2220486 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  KBP49_RS09795 (KBP49_09795) pilA 2220927..2221373 (+) 447 WP_071869805.1 pilin Machinery gene
  KBP49_RS09800 (KBP49_09800) - 2221618..2221845 (-) 228 WP_012338102.1 hypothetical protein -
  KBP49_RS09805 (KBP49_09805) pilA/pilAI 2222678..2223124 (+) 447 WP_071869804.1 pilin Machinery gene
  KBP49_RS09810 (KBP49_09810) pilB 2223964..2225697 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KBP49_RS09815 (KBP49_09815) - 2225791..2227203 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15401.80 Da        Isoelectric Point: 8.5100

>NTDB_id=485899 KBP49_RS09805 WP_071869804.1 2222678..2223124(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain AlmaEM3]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVAKSQAAAGLAEISPGRAQAEIRITEARLTTTLPADIGLRTPTPRCN
ITVNIRPDAQSTIICGLIGNAQVNGQNITLTRTNDNAAGTGGVWTCTTTLAEALRPVGCNAPSNATGG

Nucleotide


Download         Length: 447 bp        

>NTDB_id=485899 KBP49_RS09805 WP_071869804.1 2222678..2223124(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain AlmaEM3]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCCGCGATCGCTCT
GCCCATGTACCAAAATTATGTGGCCAAGTCTCAAGCCGCAGCAGGACTGGCCGAAATCTCACCAGGCCGAGCACAAGCCG
AGATCCGTATTACCGAGGCAAGGCTAACAACTACTCTTCCGGCCGATATCGGACTACGTACTCCGACCCCTCGTTGCAAC
ATTACTGTCAACATCAGGCCCGATGCTCAAAGCACAATTATATGTGGACTTATTGGTAATGCCCAAGTCAACGGTCAAAA
TATTACGTTGACTCGCACCAACGATAACGCCGCAGGCACAGGGGGGGTGTGGACCTGTACCACAACCCTTGCCGAAGCAT
TGAGACCAGTAGGCTGCAATGCTCCTAGTAATGCTACTGGTGGTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.366

95.946

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.286

94.595

0.419
  pilA Pseudomonas aeruginosa PAK

38.854

100

0.412
  pilA Acinetobacter baumannii strain A118

42.069

97.973

0.412
  pilA Vibrio parahaemolyticus RIMD 2210633

38.667

100

0.392
  pilA Ralstonia pseudosolanacearum GMI1000

39.437

95.946

0.378
  pilA Vibrio cholerae strain A1552

37.838

100

0.378
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.838

100

0.378
  pilA Vibrio cholerae C6706

37.838

100

0.378
  pilA2 Legionella pneumophila strain ERS1305867

36.842

100

0.378
  comP Acinetobacter baylyi ADP1

36.601

100

0.378
  pilA2 Legionella pneumophila str. Paris

38.571

94.595

0.365