Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IEJ03_RS06800 Genome accession   NZ_CP062005
Coordinates   1454504..1456273 (-) Length   589 a.a.
NCBI ID   WP_192036896.1    Uniprot ID   -
Organism   Halomonas sp. YLGW01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1449504..1461273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEJ03_RS06770 - 1449586..1449963 (+) 378 WP_192036890.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -
  IEJ03_RS06775 - 1450388..1451293 (-) 906 WP_192036891.1 DNA replication terminus site-binding protein -
  IEJ03_RS06780 yacG 1451306..1451533 (-) 228 WP_192036892.1 DNA gyrase inhibitor YacG -
  IEJ03_RS06785 coaE 1451629..1452240 (-) 612 WP_192036893.1 dephospho-CoA kinase -
  IEJ03_RS06790 pilD 1452237..1453100 (-) 864 WP_192036894.1 A24 family peptidase Machinery gene
  IEJ03_RS06795 pilC 1453201..1454430 (-) 1230 WP_192036895.1 type II secretion system F family protein Machinery gene
  IEJ03_RS06800 pilB 1454504..1456273 (-) 1770 WP_192036896.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IEJ03_RS06805 - 1456694..1456807 (+) 114 Protein_1309 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IEJ03_RS06810 - 1457223..1458701 (+) 1479 WP_192036897.1 O-antigen ligase family protein -
  IEJ03_RS06815 - 1458883..1460028 (-) 1146 WP_192036898.1 GGDEF domain-containing protein -
  IEJ03_RS06820 - 1460035..1460625 (-) 591 WP_192036899.1 hypothetical protein -
  IEJ03_RS06825 - 1460884..1461165 (-) 282 WP_192036900.1 hypothetical protein -

Sequence

Protein


Download         Length: 589 a.a.        Molecular weight: 64370.68 Da        Isoelectric Point: 5.1539

>NTDB_id=485901 IEJ03_RS06800 WP_192036896.1 1454504..1456273(-) (pilB) [Halomonas sp. YLGW01]
MEDTQSPTLAYPGTPAYGTPASSALRGFPLRLVSDGLLTEAAALRAVKEAEEEDVSVMRHVVENGLVPARHAALSAAWEY
GLPLIDLDGLRLASLPPANEMPEKILRKLTVMPLARSGHHLTVAVPYPSILAQLDELQFATNLTVEGVLAPVDQLGPVLE
NYLSHHENAELFGDLDDADDALEGLEYEEENTDDDKAITAGSDDAPVVKFVNKVLIDAIRRGASDIHFEPYETSYRIRLR
IDGILVEAARPPFGMRTRIAARLKVMSRLDISERRLPQDGAIKLKLSKQRSIDFRVSSLPTVFGEKLVLRILDPSSAQMG
IDALGFTPAQREMYEKALSEPQGMILVTGPTGSGKTVSLYTGINIINTGERNISTAEDPVEIKVPGVNQVNVLPKIGLTF
ASALRAFLRQDPDVVMVGEIRDLETAEIAVKASQTGHLVMSTVHTNSAAETLTRLSNMGIPAFNIASSISLIIAQRLARK
LCPHCKAPTDIPEEALRQEGFEEHEIRAATLYHPVGCRQCTLGYKGRVGIYEVVPISEAIGQLIMRNGNAMELHEQARLE
GHPDLRRSGLYKVMQGLTSLEEINRITKD

Nucleotide


Download         Length: 1770 bp        

>NTDB_id=485901 IEJ03_RS06800 WP_192036896.1 1454504..1456273(-) (pilB) [Halomonas sp. YLGW01]
ATGGAGGATACACAGTCGCCGACACTGGCCTACCCGGGAACCCCAGCCTACGGAACGCCTGCCTCATCGGCGCTGCGCGG
CTTTCCTCTTCGCCTGGTATCGGATGGCCTCCTGACCGAGGCGGCGGCACTTCGCGCCGTGAAAGAGGCGGAAGAAGAAG
ACGTCTCGGTGATGCGCCATGTGGTCGAGAACGGCTTGGTGCCAGCGCGACACGCAGCGCTCTCGGCGGCCTGGGAGTAT
GGCCTGCCGCTGATCGACCTCGATGGCCTGCGGCTGGCGAGCCTTCCTCCCGCCAACGAGATGCCGGAGAAGATTCTGCG
CAAGCTCACCGTCATGCCGCTCGCACGCAGTGGGCATCATTTGACGGTCGCCGTGCCCTACCCCTCGATACTGGCGCAGC
TCGATGAACTGCAATTTGCCACCAACCTGACGGTTGAAGGCGTGTTGGCGCCGGTGGACCAGCTGGGACCGGTCCTCGAG
AATTACCTCAGCCATCATGAAAATGCCGAACTCTTCGGTGATCTTGACGATGCCGACGATGCCCTCGAGGGATTGGAATA
CGAAGAAGAGAACACCGACGACGACAAGGCGATCACCGCGGGCAGCGACGATGCGCCGGTCGTCAAGTTCGTCAACAAGG
TGTTGATCGACGCCATCCGCCGCGGCGCCTCGGACATTCACTTCGAGCCCTACGAGACCAGCTATCGCATTCGCCTGCGC
ATCGATGGCATCCTCGTCGAGGCGGCCCGGCCGCCCTTCGGCATGCGCACCCGTATTGCCGCGCGTCTTAAGGTGATGTC
ACGCTTGGATATCTCCGAGCGTCGCCTGCCTCAGGATGGCGCCATCAAGCTGAAGTTATCTAAGCAGCGCTCCATCGACT
TCCGCGTCAGCTCCCTGCCGACGGTATTTGGCGAGAAACTGGTGCTGCGTATCCTCGACCCCAGCTCCGCGCAGATGGGC
ATCGACGCTTTGGGCTTCACCCCGGCGCAGCGGGAGATGTACGAGAAGGCGCTATCCGAGCCCCAGGGCATGATCCTGGT
CACGGGCCCCACCGGCAGCGGCAAGACCGTCTCGCTCTATACCGGGATCAATATCATCAATACCGGCGAGCGCAATATCT
CCACCGCCGAGGATCCGGTCGAGATCAAGGTACCCGGGGTCAATCAGGTCAACGTGTTGCCAAAGATCGGCCTGACCTTT
GCCAGCGCCCTGCGCGCCTTCCTTCGCCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGTGACCTGGAAACCGCGGA
GATCGCCGTCAAGGCGTCCCAGACCGGCCACCTGGTAATGTCCACTGTGCATACCAACTCTGCCGCCGAGACCCTGACCC
GCCTGTCCAATATGGGCATTCCGGCCTTCAATATTGCCAGTTCCATCTCGTTGATCATTGCGCAGCGCCTGGCGCGCAAG
CTCTGCCCGCACTGCAAGGCGCCGACCGATATCCCCGAGGAAGCCCTGCGACAGGAGGGCTTCGAGGAGCATGAAATCCG
CGCGGCCACCCTCTACCATCCGGTCGGCTGCAGGCAGTGTACCCTCGGTTACAAGGGCCGGGTCGGCATCTACGAAGTGG
TACCCATCAGTGAGGCCATCGGCCAGCTGATCATGCGCAACGGCAATGCCATGGAGCTACATGAACAGGCCCGCCTCGAG
GGCCACCCCGACCTGCGCCGCAGCGGTCTCTACAAGGTCATGCAGGGCCTGACCAGCCTGGAAGAGATCAACCGTATCAC
CAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(213-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.674

96.265

0.526
  pilB Acinetobacter baylyi ADP1

53.299

97.793

0.521
  pilB Acinetobacter baumannii D1279779

53.054

97.284

0.516
  pilB Vibrio campbellii strain DS40M4

49.46

94.397

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

48.415

96.435

0.467
  pilB Vibrio cholerae strain A1552

51.625

88.795

0.458
  pilF Neisseria gonorrhoeae MS11

49.493

83.701

0.414