Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ICJ54_RS00170 Genome accession   NZ_CP061335
Coordinates   47748..49016 (-) Length   422 a.a.
NCBI ID   WP_189121428.1    Uniprot ID   -
Organism   Pseudomonas asiatica strain C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42748..54016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICJ54_RS00155 (ICJ54_00155) - 44381..46015 (-) 1635 WP_189121427.1 SPOR domain-containing protein -
  ICJ54_RS00160 (ICJ54_00160) aroB 46031..47128 (-) 1098 WP_015272135.1 3-dehydroquinate synthase -
  ICJ54_RS00165 (ICJ54_00165) aroK 47225..47743 (-) 519 WP_015272136.1 shikimate kinase AroK -
  ICJ54_RS00170 (ICJ54_00170) pilQ 47748..49016 (-) 1269 WP_189121428.1 type IV pilus secretin PilQ Machinery gene
  ICJ54_RS00175 (ICJ54_00175) - 48940..49941 (-) 1002 WP_043936291.1 pilus assembly protein PilP -
  ICJ54_RS00180 (ICJ54_00180) - 49953..50489 (-) 537 WP_085720866.1 PilN domain-containing protein -
  ICJ54_RS00185 (ICJ54_00185) pilM 50486..51367 (-) 882 WP_139657892.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46049.89 Da        Isoelectric Point: 4.9200

>NTDB_id=481894 ICJ54_RS00170 WP_189121428.1 47748..49016(-) (pilQ) [Pseudomonas asiatica strain C1]
MKRLMKWMLAALLPFPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDAVQGSVTLRLEDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAEFAAQSVDAHVGQALDVQLQPLRRELLPIHHAKAAELAELLLASLADDGILNGRGSLSVDE
RTNTLVVYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYVENPRPGKDLFVDLGVERVGGSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVTDGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=481894 ICJ54_RS00170 WP_189121428.1 47748..49016(-) (pilQ) [Pseudomonas asiatica strain C1]
ATGAAAAGATTGATGAAATGGATGCTGGCAGCCCTGCTGCCGTTCCCGCTGGCGTCGGCAATGCCTTATCAGGGTGAACC
ACTGTCGCTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGACTATGCCGGTGTCAACCTGGTTG
CCAGCGATGCCGTGCAGGGTAGCGTCACCTTGCGCCTTGAGGATGTTCCATGGGACCAGGCGCTGGATCTGGTGCTGCGC
AGCAAGGGCTTGGCCCGGCGACAGGAGGGCAATGTGCTGTTGGTGGCGCCCGCGGCGGAGTTTGCCGCGCAGTCTGTCGA
TGCCCATGTGGGCCAGGCGCTGGATGTACAGTTGCAGCCGTTGCGTCGGGAGCTGTTGCCCATTCATCACGCCAAGGCCG
CCGAGCTGGCAGAGCTGCTGCTGGCGAGCCTGGCGGATGACGGCATCCTCAACGGTCGGGGCAGCCTGAGTGTCGATGAG
CGCACCAATACGCTGGTGGTGTACCAGCCGGCGGACCGCCTTGCCGAACTGAGGCAGCTGGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCGATCGAGGCACGTATCGTCGAGGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGTG
GGCCGCTGTATGTCGAGAACCCGCGCCCGGGCAAGGATCTGTTCGTCGATCTCGGGGTGGAGCGGGTGGGCGGTAGCATC
GGTCTGGGCTTGTTGCGTGGCGATGTGCTGCTCGACCTGGAACTCAGTGCCATGGAAAAGAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCGTACCAGGAAACCA
GCAAGAGCGGCGCCACCTCGGTAGCGTTCCGCGAAGCCTCGTTGTCGCTGGAAGTGACCCCGCAGATCACTCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCGAAGGTTCGCGTGACGGACGGAGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.611
  pilQ Acinetobacter baumannii strain A118

42.14

100

0.457
  pilQ Acinetobacter baumannii D1279779

42.14

100

0.457
  comQ Acinetobacter baylyi ADP1

42.257

100

0.453
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.014

100

0.422
  pilQ Vibrio cholerae strain A1552

41.014

100

0.422
  pilQ Legionella pneumophila strain Lp02

42.344

99.052

0.419
  pilQ Legionella pneumophila strain ERS1305867

42.344

99.052

0.419
  pilQ Vibrio campbellii strain DS40M4

40.741

100

0.417
  pilQ Neisseria gonorrhoeae strain FA1090

36.993

99.289

0.367
  pilQ Neisseria meningitidis M1080

36.277

99.289

0.36