Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   D8K17_RS11700 Genome accession   NZ_CP061322
Coordinates   2284111..2285049 (-) Length   312 a.a.
NCBI ID   WP_010906320.1    Uniprot ID   A0AAC9W673
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain S50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2244426..2284163 2284111..2285049 flank -52


Gene organization within MGE regions


Location: 2244426..2285049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8K17_RS11415 (D8K17_011435) - 2244426..2245163 (-) 738 WP_010906311.1 metal ABC transporter ATP-binding protein -
  D8K17_RS11420 (D8K17_011440) - 2245340..2246182 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  D8K17_RS11425 (D8K17_011445) - 2246179..2246616 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  D8K17_RS11430 (D8K17_011450) comGG 2246697..2246981 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  D8K17_RS11435 (D8K17_011455) comGF 2247020..2247466 (-) 447 WP_031296844.1 competence type IV pilus minor pilin ComGF Machinery gene
  D8K17_RS11440 (D8K17_011460) comGE 2247429..2247725 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  D8K17_RS11445 (D8K17_011465) comGD 2247697..2248113 (-) 417 WP_021216554.1 competence type IV pilus minor pilin ComGD Machinery gene
  D8K17_RS11450 (D8K17_011470) comGC 2248088..2248357 (-) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  D8K17_RS11455 (D8K17_011475) - 2248504..2249028 (-) 525 WP_014570795.1 GNAT family N-acetyltransferase -
  D8K17_RS11460 (D8K17_011480) - 2249107..2249886 (-) 780 WP_058147788.1 peptidoglycan amidohydrolase family protein -
  D8K17_RS11465 (D8K17_011485) - 2249886..2250185 (-) 300 WP_031559226.1 phage holin -
  D8K17_RS11470 (D8K17_011490) - 2250198..2250548 (-) 351 WP_023349296.1 hypothetical protein -
  D8K17_RS14055 - 2250568..2252148 (-) 1581 WP_223203828.1 hypothetical protein -
  D8K17_RS11480 (D8K17_011500) - 2252126..2252299 (-) 174 WP_032941620.1 hypothetical protein -
  D8K17_RS11485 (D8K17_011505) - 2252299..2253420 (-) 1122 WP_031297171.1 hypothetical protein -
  D8K17_RS11490 (D8K17_011510) - 2253437..2255230 (-) 1794 WP_031297170.1 phage tail protein -
  D8K17_RS11495 (D8K17_011515) - 2255227..2256747 (-) 1521 WP_058147722.1 distal tail protein Dit -
  D8K17_RS11500 (D8K17_011520) - 2256757..2259366 (-) 2610 WP_023349195.1 hypothetical protein -
  D8K17_RS11505 (D8K17_011525) - 2259356..2260063 (-) 708 WP_014570560.1 Gp15 family bacteriophage protein -
  D8K17_RS11510 (D8K17_011530) - 2260079..2260486 (-) 408 WP_003131323.1 hypothetical protein -
  D8K17_RS11515 (D8K17_011535) - 2260543..2261019 (-) 477 WP_014570559.1 phage tail tube protein -
  D8K17_RS11520 (D8K17_011540) - 2261030..2261464 (-) 435 WP_003131321.1 minor capsid protein -
  D8K17_RS11525 (D8K17_011545) - 2261464..2261793 (-) 330 WP_003131320.1 hypothetical protein -
  D8K17_RS11530 (D8K17_011550) - 2261790..2262134 (-) 345 WP_003131319.1 putative minor capsid protein -
  D8K17_RS11535 (D8K17_011555) - 2262124..2262525 (-) 402 WP_014570556.1 hypothetical protein -
  D8K17_RS11540 (D8K17_011560) - 2262599..2262835 (-) 237 WP_023349196.1 Ig-like domain-containing protein -
  D8K17_RS11545 (D8K17_011565) - 2262864..2263781 (-) 918 WP_023349197.1 phage capsid protein -
  D8K17_RS11550 (D8K17_011570) - 2263796..2264848 (-) 1053 WP_023349198.1 XkdF-like putative serine protease domain-containing protein -
  D8K17_RS11555 (D8K17_011575) - 2264864..2265694 (-) 831 WP_023349199.1 phage minor head protein -
  D8K17_RS11560 (D8K17_011580) - 2265687..2267216 (-) 1530 WP_023349200.1 phage portal protein -
  D8K17_RS11565 (D8K17_011585) terL 2267229..2268680 (-) 1452 WP_023349201.1 phage terminase large subunit -
  D8K17_RS11570 (D8K17_011590) - 2268661..2269158 (-) 498 WP_023349202.1 hypothetical protein -
  D8K17_RS11575 (D8K17_011595) - 2269187..2270344 (-) 1158 WP_023349203.1 DNA modification methylase -
  D8K17_RS11585 (D8K17_011605) - 2270821..2271243 (-) 423 WP_023349204.1 RinA family protein -
  D8K17_RS11590 (D8K17_011610) - 2271320..2271628 (-) 309 WP_023349205.1 hypothetical protein -
  D8K17_RS11595 (D8K17_011615) - 2271757..2271951 (-) 195 WP_023349206.1 DUF1660 domain-containing protein -
  D8K17_RS11600 (D8K17_011620) - 2271948..2272166 (-) 219 WP_014570803.1 hypothetical protein -
  D8K17_RS11605 (D8K17_011625) - 2272213..2272458 (-) 246 WP_023349207.1 hypothetical protein -
  D8K17_RS11610 (D8K17_011630) - 2272480..2272839 (-) 360 WP_023349208.1 hypothetical protein -
  D8K17_RS11615 (D8K17_011635) dut 2272842..2273261 (-) 420 WP_023349209.1 dUTP diphosphatase -
  D8K17_RS11620 (D8K17_011640) - 2273258..2273563 (-) 306 WP_023349170.1 hypothetical protein -
  D8K17_RS11625 (D8K17_011645) - 2273560..2274063 (-) 504 WP_023349171.1 DUF1642 domain-containing protein -
  D8K17_RS11630 (D8K17_011650) - 2274056..2274451 (-) 396 WP_058147723.1 YopX family protein -
  D8K17_RS11635 (D8K17_011655) - 2274957..2275367 (-) 411 WP_014570810.1 hypothetical protein -
  D8K17_RS11640 (D8K17_011660) - 2275380..2275622 (-) 243 WP_023349173.1 L-rhamnose isomerase -
  D8K17_RS11645 (D8K17_011665) - 2275615..2276538 (-) 924 WP_023349174.1 phage replisome organizer N-terminal domain-containing protein -
  D8K17_RS11650 (D8K17_011670) - 2276802..2277728 (-) 927 WP_014570813.1 RecT family recombinase -
  D8K17_RS11655 (D8K17_011675) - 2277725..2278558 (-) 834 WP_023349175.1 hypothetical protein -
  D8K17_RS11660 (D8K17_011680) - 2278661..2278909 (-) 249 WP_014570815.1 hypothetical protein -
  D8K17_RS14135 - 2278923..2279045 (-) 123 WP_023164646.1 hypothetical protein -
  D8K17_RS11665 (D8K17_011685) - 2279042..2279224 (-) 183 WP_023349176.1 hypothetical protein -
  D8K17_RS11670 (D8K17_011690) - 2279288..2279497 (-) 210 WP_023349177.1 helix-turn-helix transcriptional regulator -
  D8K17_RS11675 (D8K17_011695) - 2279688..2280110 (+) 423 WP_023349178.1 helix-turn-helix transcriptional regulator -
  D8K17_RS11680 (D8K17_011700) - 2280121..2280705 (+) 585 WP_023349179.1 hypothetical protein -
  D8K17_RS11685 (D8K17_011705) - 2280759..2281421 (+) 663 WP_023349180.1 SHOCT domain-containing protein -
  D8K17_RS11690 (D8K17_011710) - 2281536..2282993 (+) 1458 WP_058147724.1 recombinase family protein -
  D8K17_RS11695 (D8K17_011720) comGB 2283144..2284217 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  D8K17_RS11700 (D8K17_011725) comGA 2284111..2285049 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35719.89 Da        Isoelectric Point: 6.1804

>NTDB_id=481864 D8K17_RS11700 WP_010906320.1 2284111..2285049(-) (comGA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDQELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
INIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=481864 D8K17_RS11700 WP_010906320.1 2284111..2285049(-) (comGA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATCAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCAATATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.603

100

0.766
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.09

99.679

0.519
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519
  comYA Streptococcus mutans UA140

51.923

100

0.519
  comYA Streptococcus mutans UA159

51.923

100

0.519