Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   J5V74_RS01280 Genome accession   NZ_CP071947
Coordinates   282385..282849 (-) Length   154 a.a.
NCBI ID   WP_023086642.1    Uniprot ID   Q8KQ34
Organism   Pseudomonas aeruginosa strain 2020HL-00861     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 277385..287849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5V74_RS01265 (J5V74_01265) nadC 279885..280733 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J5V74_RS01275 (J5V74_01275) - 280914..282227 (-) 1314 WP_223218619.1 O-antigen ligase -
  J5V74_RS01280 (J5V74_01280) pilA/pilAI 282385..282849 (-) 465 WP_023086642.1 pilin Machinery gene
  J5V74_RS01285 (J5V74_01285) pilB 283080..284780 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J5V74_RS01290 (J5V74_01290) pilC 284784..286001 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  J5V74_RS01295 (J5V74_01295) pilD 286002..286874 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  J5V74_RS01300 (J5V74_01300) coaE 286871..287482 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  J5V74_RS01305 (J5V74_01305) yacG 287479..287679 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16021.32 Da        Isoelectric Point: 8.9987

>NTDB_id=479727 J5V74_RS01280 WP_023086642.1 282385..282849(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2020HL-00861]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGSGKGQIKIDKADTATPEISGTLGGSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=479727 J5V74_RS01280 WP_023086642.1 282385..282849(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 2020HL-00861]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTAGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACTGGTAGCGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCGGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8KQ34

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.161

100

0.455
  pilA Acinetobacter baumannii strain A118

46.207

94.156

0.435
  pilA Pseudomonas aeruginosa PAK

41.772

100

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

36.471

100

0.403
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.514

96.104

0.37
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.255

96.753

0.37
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364