Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   JZM58_RS25500 Genome accession   NZ_CP071797
Coordinates   5737042..5737458 (-) Length   138 a.a.
NCBI ID   WP_011335978.1    Uniprot ID   A0ABT5PXS0
Organism   Pseudomonas fluorescens strain YK-310     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5732042..5742458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JZM58_RS25485 (JZM58_25485) - 5732961..5735912 (+) 2952 WP_207983960.1 DUF748 domain-containing protein -
  JZM58_RS25490 (JZM58_25490) - 5735912..5736208 (+) 297 WP_007959599.1 DUF2845 domain-containing protein -
  JZM58_RS25495 (JZM58_25495) - 5736375..5736731 (-) 357 WP_085608680.1 BON domain-containing protein -
  JZM58_RS25500 (JZM58_25500) pilA/pilAI 5737042..5737458 (-) 417 WP_011335978.1 pilin Machinery gene
  JZM58_RS25505 (JZM58_25505) pilB 5737685..5739385 (+) 1701 WP_207983961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JZM58_RS25510 (JZM58_25510) pilC 5739388..5740605 (+) 1218 WP_207983962.1 type II secretion system F family protein Machinery gene
  JZM58_RS25515 (JZM58_25515) pilD 5740607..5741479 (+) 873 WP_207983963.1 A24 family peptidase Machinery gene
  JZM58_RS25520 (JZM58_25520) coaE 5741476..5742099 (+) 624 WP_207983964.1 dephospho-CoA kinase -
  JZM58_RS25525 (JZM58_25525) yacG 5742096..5742296 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14069.14 Da        Isoelectric Point: 8.4918

>NTDB_id=478927 JZM58_RS25500 WP_011335978.1 5737042..5737458(-) (pilA/pilAI) [Pseudomonas fluorescens strain YK-310]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARAKVTAGIAEASALKVNFEDVINAGSTDPSITNVNAGTTATQNCT
LAASGKVADGTGTITCTLLNAPAPVLGQTVTLTRTAAAGWSCKFSGSKDYSTTSCPGV

Nucleotide


Download         Length: 417 bp        

>NTDB_id=478927 JZM58_RS25500 WP_011335978.1 5737042..5737458(-) (pilA/pilAI) [Pseudomonas fluorescens strain YK-310]
ATGAAGAAACAACAAGGTTTCACTCTGATCGAGCTGCTGATCGTTGTGGCGATCATCGGGATTCTGGCGACGATTGCCTT
GCCCCAGTACTCCAAATATCAGGCGCGGGCCAAGGTCACGGCGGGGATTGCCGAAGCGTCGGCGCTTAAGGTCAACTTCG
AAGACGTCATCAATGCCGGCTCTACTGATCCGAGCATTACTAACGTCAATGCGGGTACGACTGCGACACAAAATTGCACG
CTGGCGGCGAGCGGTAAAGTTGCCGACGGCACAGGGACCATCACCTGCACCTTGCTCAACGCTCCGGCGCCTGTGTTGGG
GCAAACCGTCACCCTGACCCGTACGGCAGCAGCAGGTTGGTCGTGCAAGTTCTCGGGTTCGAAAGACTACTCGACCACCA
GTTGCCCGGGCGTTTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.526

99.275

0.442
  pilA Pseudomonas aeruginosa PAK

40.541

100

0.435
  comP Acinetobacter baylyi ADP1

39.073

100

0.428
  pilA Acinetobacter baumannii strain A118

43.651

91.304

0.399
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.441

98.551

0.399
  pilA Vibrio cholerae C6706

36.62

100

0.377
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.62

100

0.377
  pilA Vibrio cholerae strain A1552

36.62

100

0.377
  pilA Vibrio parahaemolyticus RIMD 2210633

42.975

87.681

0.377
  pilA Ralstonia pseudosolanacearum GMI1000

43.59

84.783

0.37