Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J1G53_RS00915 Genome accession   NZ_CP071490
Coordinates   181854..183239 (+) Length   461 a.a.
NCBI ID   WP_207342514.1    Uniprot ID   -
Organism   Glaesserella parasuis strain GHP1808     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 176854..188239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G53_RS00890 (J1G53_00890) - 177128..178099 (+) 972 WP_012621529.1 IS110 family transposase -
  J1G53_RS00895 (J1G53_00895) gmk 178168..178794 (+) 627 WP_010787010.1 guanylate kinase -
  J1G53_RS00900 (J1G53_00900) radA/sms 178847..180223 (-) 1377 WP_021114521.1 DNA repair protein RadA Machinery gene
  J1G53_RS00905 (J1G53_00905) pilA 180411..180863 (+) 453 WP_010787008.1 pilin Machinery gene
  J1G53_RS00910 (J1G53_00910) - 181136..181501 (+) 366 WP_015940027.1 endonuclease domain-containing protein -
  J1G53_RS00915 (J1G53_00915) pilB 181854..183239 (+) 1386 WP_207342514.1 GspE/PulE family protein Machinery gene
  J1G53_RS00920 (J1G53_00920) pilC 183232..184428 (+) 1197 WP_160434283.1 type II secretion system F family protein Machinery gene
  J1G53_RS00925 (J1G53_00925) - 184425..185093 (+) 669 WP_207342515.1 prepilin peptidase -
  J1G53_RS00930 (J1G53_00930) dapE 185177..186310 (+) 1134 WP_005711772.1 succinyl-diaminopimelate desuccinylase -
  J1G53_RS00935 (J1G53_00935) clpX 186384..187634 (-) 1251 WP_160429045.1 ATP-dependent protease ATP-binding subunit ClpX Regulator
  J1G53_RS00940 (J1G53_00940) clpP 187641..188222 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52744.60 Da        Isoelectric Point: 5.1467

>NTDB_id=477256 J1G53_RS00915 WP_207342514.1 181854..183239(+) (pilB) [Glaesserella parasuis strain GHP1808]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPIYEESELAFVEQEQEQLNLNDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=477256 J1G53_RS00915 WP_207342514.1 181854..183239(+) (pilB) [Glaesserella parasuis strain GHP1808]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCAGTGGTGATTGCTTCTGATGAA
CTGAAATACTTGCTTAACGCTCTCTCGCCTGAACAACAGCCCATTTATGAAGAGAGCGAGCTGGCTTTTGTTGAACAGGA
GCAAGAACAGCTCAACCTAAACGATCCGATTATTCAACTGCTAGATAATCTCTTTAAATTTTGTCTAGCTCAGAATGCGT
CCGACATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGCCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTAGATTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

98.265

100

0.983
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.798

98.915

0.562
  pilB Vibrio cholerae strain A1552

38.554

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.085

100

0.408
  pilB Acinetobacter baumannii D1279779

36.381

100

0.406
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.889

100

0.395
  pilB Legionella pneumophila strain ERS1305867

40.227

95.445

0.384
  pilF Neisseria gonorrhoeae MS11

36.211

100

0.373