Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J1G53_RS00905 Genome accession   NZ_CP071490
Coordinates   180411..180863 (+) Length   150 a.a.
NCBI ID   WP_010787008.1    Uniprot ID   B8F829
Organism   Glaesserella parasuis strain GHP1808     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 175411..185863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G53_RS00885 (J1G53_00885) gpt 176193..176672 (+) 480 WP_010787011.1 xanthine phosphoribosyltransferase -
  J1G53_RS00890 (J1G53_00890) - 177128..178099 (+) 972 WP_012621529.1 IS110 family transposase -
  J1G53_RS00895 (J1G53_00895) gmk 178168..178794 (+) 627 WP_010787010.1 guanylate kinase -
  J1G53_RS00900 (J1G53_00900) radA/sms 178847..180223 (-) 1377 WP_021114521.1 DNA repair protein RadA Machinery gene
  J1G53_RS00905 (J1G53_00905) pilA 180411..180863 (+) 453 WP_010787008.1 pilin Machinery gene
  J1G53_RS00910 (J1G53_00910) - 181136..181501 (+) 366 WP_015940027.1 endonuclease domain-containing protein -
  J1G53_RS00915 (J1G53_00915) pilB 181854..183239 (+) 1386 WP_207342514.1 GspE/PulE family protein Machinery gene
  J1G53_RS00920 (J1G53_00920) pilC 183232..184428 (+) 1197 WP_160434283.1 type II secretion system F family protein Machinery gene
  J1G53_RS00925 (J1G53_00925) - 184425..185093 (+) 669 WP_207342515.1 prepilin peptidase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 16138.74 Da        Isoelectric Point: 9.0811

>NTDB_id=477255 J1G53_RS00905 WP_010787008.1 180411..180863(+) (pilA) [Glaesserella parasuis strain GHP1808]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVRADKSDVPDAKYLKSISVASGVITVAGKGNIDGYGYTMTPKFANNNITWATSCQGADISLFPENFCRQ

Nucleotide


Download         Length: 453 bp        

>NTDB_id=477255 J1G53_RS00905 WP_010787008.1 180411..180863(+) (pilA) [Glaesserella parasuis strain GHP1808]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCAGCTCTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCGAGCAGATAAATCCGATGTCCCTGACGCGAAATACCTTAAATCTATTTCCGTCGCTTCGGGGGTAATCACCGT
GGCAGGTAAAGGCAATATTGATGGCTACGGCTACACAATGACTCCGAAATTCGCTAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGAGAATTTCTGTAGGCAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8F829

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

98.667

100

0.987
  pilA Haemophilus influenzae Rd KW20

54.054

98.667

0.533
  pilA Haemophilus influenzae 86-028NP

53.378

98.667

0.527