Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J1G47_RS01105 Genome accession   NZ_CP071487
Coordinates   208668..210053 (+) Length   461 a.a.
NCBI ID   WP_010787006.1    Uniprot ID   -
Organism   Glaesserella parasuis strain YHP1818     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 203668..215053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G47_RS01080 (J1G47_01080) gpt 204376..204855 (+) 480 WP_160403664.1 xanthine phosphoribosyltransferase -
  J1G47_RS01085 (J1G47_01085) gmk 204982..205608 (+) 627 WP_010787010.1 guanylate kinase -
  J1G47_RS01090 (J1G47_01090) radA/sms 205661..207037 (-) 1377 WP_160403706.1 DNA repair protein RadA Machinery gene
  J1G47_RS01095 (J1G47_01095) pilA 207225..207677 (+) 453 WP_010787008.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J1G47_RS01100 (J1G47_01100) - 207950..208315 (+) 366 WP_015940027.1 endonuclease domain-containing protein -
  J1G47_RS01105 (J1G47_01105) pilB 208668..210053 (+) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  J1G47_RS01110 (J1G47_01110) pilC 210046..211242 (+) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  J1G47_RS01115 (J1G47_01115) - 211239..211907 (+) 669 WP_010787005.1 prepilin peptidase -
  J1G47_RS01120 (J1G47_01120) dapE 211991..213124 (+) 1134 WP_207326695.1 succinyl-diaminopimelate desuccinylase -
  J1G47_RS01125 (J1G47_01125) clpX 213198..214448 (-) 1251 WP_005711769.1 ATP-dependent protease ATP-binding subunit ClpX Regulator
  J1G47_RS01130 (J1G47_01130) clpP 214455..215036 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52808.65 Da        Isoelectric Point: 5.3881

>NTDB_id=477142 J1G47_RS01105 WP_010787006.1 208668..210053(+) (pilB) [Glaesserella parasuis strain YHP1818]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPIYEESELAFVEQEQEQLNLNDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNRSIQLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGVGC
DECYQGYRGRIGVYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNETEVDRVLGYE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=477142 J1G47_RS01105 WP_010787006.1 208668..210053(+) (pilB) [Glaesserella parasuis strain YHP1818]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCAGTGGTGATTGCTTCTGATGAA
CTGAAATACTTGCTTAACGCTCTCTCGCCTGAACAACAGCCCATTTATGAAGAGAGCGAGCTGGCTTTTGTTGAACAGGA
GCAAGAACAGCTCAACCTAAACGATCCGATTATTCAACTGCTAGATAATCTCTTTAAATTTTGTCTAGCTCAGAATGCGT
CCGACATTCATATTGAGCCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGCGTTTTGCACCTGTATAAAAGC
CTATCTATTCAGCTGGCAAGCCGACTAATTTCACGGATAAAACTACTGGCAAAGCTAGATATTAGCGAATTACGCCAGCC
ACAAGACGGGCAATTTAGTTTTACCACCGCCCTTGCGGATACCCTTGATTTTCGGGTGTCTAGCCTGCCGACGATCTACG
GTGAAAAACTGGTATTACGCTTACAAAAAAACAAGCCGACCAGTTTTGATTTTCTACAACTTGGCTTTAATCCACAACAG
CAAACCACACTGCTCAACGCTCTGAAACAACCACAGGGGCTGATTTTAGTGACCGGACCAACGGGCAGTGGCAAAAGCAT
CACTCTGTACAGTGCCTTAAACTATCTTAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCGGTGGAAATCGAGA
TTGACGGCTTAATTCAAACCCAAGTTAATCGCAGTATTCAACTGGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCTGACATCATTATGTTAGGCGAAATTCGAGATGAAGAGAGTGCCAAAATGGCGTTACGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCGACCTTGCATACCAACGACGCACTCTCTGCGGTCGAACGCTTGTTGCAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGTAAAAAGTGTCGTGGAGTAGGCTGT
GACGAGTGCTATCAAGGCTATAGAGGGCGGATCGGGGTTTACCAATGTTTAAGCAGAACGGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCAGCCTACGAGAGAGTGCTAAACAGAAAATTGAACAACAACTCACCAATGAAACCGAAG
TGGATAGGGTATTAGGTTATGAATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

100

100

1
  pilB Haemophilus influenzae Rd KW20

58.093

97.831

0.568
  pilB Haemophilus influenzae 86-028NP

57.456

98.915

0.568
  pilB Vibrio cholerae strain A1552

38.2

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.13

100

0.41
  pilB Legionella pneumophila strain ERS1305867

38.211

100

0.408
  pilB Vibrio campbellii strain DS40M4

38.923

100

0.408
  pilB Acinetobacter baumannii D1279779

36.187

100

0.403
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.248

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.421

100

0.375
  pilF Thermus thermophilus HB27

35.01

100

0.362