Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J1G47_RS01095 Genome accession   NZ_CP071487
Coordinates   207225..207677 (+) Length   150 a.a.
NCBI ID   WP_010787008.1    Uniprot ID   B8F829
Organism   Glaesserella parasuis strain YHP1818     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 202225..212677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G47_RS01075 (J1G47_01075) - 202741..204195 (-) 1455 WP_075605845.1 aminoacyl-histidine dipeptidase -
  J1G47_RS01080 (J1G47_01080) gpt 204376..204855 (+) 480 WP_160403664.1 xanthine phosphoribosyltransferase -
  J1G47_RS01085 (J1G47_01085) gmk 204982..205608 (+) 627 WP_010787010.1 guanylate kinase -
  J1G47_RS01090 (J1G47_01090) radA/sms 205661..207037 (-) 1377 WP_160403706.1 DNA repair protein RadA Machinery gene
  J1G47_RS01095 (J1G47_01095) pilA 207225..207677 (+) 453 WP_010787008.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J1G47_RS01100 (J1G47_01100) - 207950..208315 (+) 366 WP_015940027.1 endonuclease domain-containing protein -
  J1G47_RS01105 (J1G47_01105) pilB 208668..210053 (+) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  J1G47_RS01110 (J1G47_01110) pilC 210046..211242 (+) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  J1G47_RS01115 (J1G47_01115) - 211239..211907 (+) 669 WP_010787005.1 prepilin peptidase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 16138.74 Da        Isoelectric Point: 9.0811

>NTDB_id=477141 J1G47_RS01095 WP_010787008.1 207225..207677(+) (pilA) [Glaesserella parasuis strain YHP1818]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVRADKSDVPDAKYLKSISVASGVITVAGKGNIDGYGYTMTPKFANNNITWATSCQGADISLFPENFCRQ

Nucleotide


Download         Length: 453 bp        

>NTDB_id=477141 J1G47_RS01095 WP_010787008.1 207225..207677(+) (pilA) [Glaesserella parasuis strain YHP1818]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTAATGATCGTGATCGCCATCATTGC
CATTTTAGCAACAATAGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCAGCTCTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCGAGCGGATAAATCCGATGTCCCTGACGCGAAATACCTTAAATCTATTTCCGTCGCTTCGGGGGTAATCACCGT
GGCAGGTAAAGGCAATATTGATGGCTACGGCTACACAATGACTCCGAAATTCGCTAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGAGAATTTCTGTAGGCAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8F829

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

98.667

100

0.987
  pilA Haemophilus influenzae Rd KW20

54.054

98.667

0.533
  pilA Haemophilus influenzae 86-028NP

53.378

98.667

0.527