Detailed information
Bioinformatically predicted
Overview
| Name | ssb | Type | Machinery gene |
| Locus tag | HUG14_RS08005 | Genome accession | NZ_CP060580 |
| Coordinates | 1640318..1640752 (+) | Length | 144 a.a. |
| NCBI ID | WP_187398260.1 | Uniprot ID | - |
| Organism | Lactococcus lactis strain K_LL005 | ||
| Function | ssDNA binding (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1631048..1675763 | 1640318..1640752 | within | 0 |
Gene organization within MGE regions
Location: 1631048..1675763
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUG14_RS07925 (HUG14_07925) | - | 1631048..1631710 (+) | 663 | WP_012897517.1 | ABC transporter ATP-binding protein | - |
| HUG14_RS07930 (HUG14_07930) | - | 1631868..1632743 (+) | 876 | WP_012897518.1 | LysR family transcriptional regulator | - |
| HUG14_RS07935 (HUG14_07935) | - | 1632983..1633657 (+) | 675 | WP_003131377.1 | GTP pyrophosphokinase family protein | - |
| HUG14_RS07940 (HUG14_07940) | rplS | 1633798..1634142 (+) | 345 | WP_058211858.1 | 50S ribosomal protein L19 | - |
| HUG14_RS07945 (HUG14_07945) | - | 1634261..1635391 (-) | 1131 | WP_187398249.1 | site-specific integrase | - |
| HUG14_RS07950 (HUG14_07950) | - | 1635538..1636293 (-) | 756 | WP_187398250.1 | hypothetical protein | - |
| HUG14_RS07955 (HUG14_07955) | - | 1636352..1636939 (-) | 588 | WP_187398251.1 | hypothetical protein | - |
| HUG14_RS07960 (HUG14_07960) | - | 1636950..1637480 (-) | 531 | WP_187398252.1 | XRE family transcriptional regulator | - |
| HUG14_RS07965 (HUG14_07965) | - | 1637722..1637925 (+) | 204 | WP_187398253.1 | hypothetical protein | - |
| HUG14_RS07970 (HUG14_07970) | - | 1637940..1638167 (-) | 228 | WP_187398254.1 | hypothetical protein | - |
| HUG14_RS07975 (HUG14_07975) | - | 1638272..1638502 (+) | 231 | WP_187398255.1 | helix-turn-helix transcriptional regulator | - |
| HUG14_RS07980 (HUG14_07980) | - | 1638514..1639245 (+) | 732 | WP_187398256.1 | ORF6C domain-containing protein | - |
| HUG14_RS07985 (HUG14_07985) | - | 1639385..1639636 (+) | 252 | WP_033900176.1 | hypothetical protein | - |
| HUG14_RS07990 (HUG14_07990) | - | 1639724..1639888 (+) | 165 | WP_187398257.1 | hypothetical protein | - |
| HUG14_RS07995 (HUG14_07995) | - | 1639885..1640094 (+) | 210 | WP_187398258.1 | hypothetical protein | - |
| HUG14_RS08000 (HUG14_08000) | - | 1640096..1640308 (+) | 213 | WP_187398259.1 | hypothetical protein | - |
| HUG14_RS08005 (HUG14_08005) | ssb | 1640318..1640752 (+) | 435 | WP_187398260.1 | single-stranded DNA-binding protein | Machinery gene |
| HUG14_RS08010 (HUG14_08010) | - | 1640763..1641605 (+) | 843 | WP_187398261.1 | DnaD domain protein | - |
| HUG14_RS08015 (HUG14_08015) | - | 1641609..1642007 (+) | 399 | WP_187398262.1 | hypothetical protein | - |
| HUG14_RS08020 (HUG14_08020) | - | 1642004..1642513 (+) | 510 | WP_187398263.1 | hypothetical protein | - |
| HUG14_RS08025 (HUG14_08025) | - | 1642649..1643539 (+) | 891 | WP_262384323.1 | DUF1642 domain-containing protein | - |
| HUG14_RS08030 (HUG14_08030) | - | 1643656..1644216 (-) | 561 | WP_187398264.1 | hypothetical protein | - |
| HUG14_RS08035 (HUG14_08035) | - | 1644251..1644475 (+) | 225 | WP_262384324.1 | hypothetical protein | - |
| HUG14_RS08040 (HUG14_08040) | - | 1644603..1645262 (-) | 660 | WP_187398265.1 | DUF4145 domain-containing protein | - |
| HUG14_RS11690 | - | 1645393..1645536 (+) | 144 | WP_262384325.1 | hypothetical protein | - |
| HUG14_RS08050 (HUG14_08050) | - | 1645615..1645773 (+) | 159 | WP_187398266.1 | DUF1660 family phage protein | - |
| HUG14_RS08055 (HUG14_08055) | - | 1645773..1646003 (+) | 231 | WP_187398267.1 | hypothetical protein | - |
| HUG14_RS08060 (HUG14_08060) | - | 1646045..1646428 (+) | 384 | WP_187398268.1 | RusA family crossover junction endodeoxyribonuclease | - |
| HUG14_RS08065 (HUG14_08065) | - | 1646548..1646844 (+) | 297 | WP_187398269.1 | hypothetical protein | - |
| HUG14_RS08070 (HUG14_08070) | - | 1646888..1647181 (+) | 294 | WP_187398270.1 | DUF1359 domain-containing protein | - |
| HUG14_RS08075 (HUG14_08075) | - | 1647464..1647805 (+) | 342 | WP_023189566.1 | hypothetical protein | - |
| HUG14_RS08080 (HUG14_08080) | - | 1647897..1648184 (+) | 288 | WP_187398271.1 | HNH endonuclease | - |
| HUG14_RS11695 | - | 1648327..1648923 (+) | 597 | WP_262384326.1 | helix-turn-helix transcriptional regulator | - |
| HUG14_RS08090 (HUG14_08090) | - | 1648920..1650653 (+) | 1734 | WP_187398272.1 | terminase large subunit domain-containing protein | - |
| HUG14_RS08095 (HUG14_08095) | - | 1650728..1651858 (+) | 1131 | WP_187398273.1 | phage portal protein | - |
| HUG14_RS08100 (HUG14_08100) | - | 1651845..1652606 (+) | 762 | WP_187398274.1 | head maturation protease, ClpP-related | - |
| HUG14_RS08105 (HUG14_08105) | - | 1652617..1653765 (+) | 1149 | WP_187398275.1 | phage major capsid protein | - |
| HUG14_RS08110 (HUG14_08110) | - | 1653827..1654147 (+) | 321 | WP_262384327.1 | hypothetical protein | - |
| HUG14_RS08115 (HUG14_08115) | - | 1654140..1654517 (+) | 378 | WP_187398276.1 | phage head-tail adapter protein | - |
| HUG14_RS08120 (HUG14_08120) | - | 1654517..1654927 (+) | 411 | WP_023189575.1 | hypothetical protein | - |
| HUG14_RS08125 (HUG14_08125) | - | 1654920..1655321 (+) | 402 | WP_187398277.1 | hypothetical protein | - |
| HUG14_RS08130 (HUG14_08130) | - | 1655334..1655915 (+) | 582 | WP_187398278.1 | major tail protein | - |
| HUG14_RS08135 (HUG14_08135) | - | 1655991..1656248 (+) | 258 | WP_187398279.1 | hypothetical protein | - |
| HUG14_RS08140 (HUG14_08140) | gpG | 1656265..1656573 (+) | 309 | WP_058210728.1 | phage tail assembly chaperone G | - |
| HUG14_RS08145 (HUG14_08145) | - | 1656600..1656758 (+) | 159 | WP_187398280.1 | hypothetical protein | - |
| HUG14_RS08150 (HUG14_08150) | - | 1656998..1657477 (+) | 480 | WP_222867523.1 | hypothetical protein | - |
| HUG14_RS08155 (HUG14_08155) | - | 1657635..1661249 (+) | 3615 | WP_187398281.1 | phage tail tape measure protein | - |
| HUG14_RS08160 (HUG14_08160) | - | 1661259..1661603 (+) | 345 | WP_187398282.1 | hypothetical protein | - |
| HUG14_RS08165 (HUG14_08165) | - | 1661603..1662484 (+) | 882 | WP_187398283.1 | phage tail domain-containing protein | - |
| HUG14_RS08170 (HUG14_08170) | - | 1662484..1663614 (+) | 1131 | WP_187398284.1 | siphovirus ReqiPepy6 Gp37-like family protein | - |
| HUG14_RS08175 (HUG14_08175) | - | 1663623..1664912 (+) | 1290 | WP_187398285.1 | pyocin knob domain-containing protein | - |
| HUG14_RS08180 (HUG14_08180) | - | 1664997..1665422 (+) | 426 | WP_033900142.1 | phage holin family protein | - |
| HUG14_RS08185 (HUG14_08185) | - | 1665412..1665639 (+) | 228 | WP_187398286.1 | phage holin | - |
| HUG14_RS08190 (HUG14_08190) | - | 1665636..1666976 (+) | 1341 | WP_187398287.1 | LysM peptidoglycan-binding domain-containing protein | - |
| HUG14_RS11530 | - | 1667006..1667203 (-) | 198 | WP_222867531.1 | MmcQ/YjbR family DNA-binding protein | - |
| HUG14_RS08195 (HUG14_08195) | - | 1667273..1667569 (-) | 297 | WP_262384328.1 | membrane lipoprotein lipid attachment site-containing protein | - |
| HUG14_RS08200 (HUG14_08200) | - | 1669304..1669504 (+) | 201 | WP_014570681.1 | cold-shock protein | - |
| HUG14_RS08205 (HUG14_08205) | - | 1669979..1670359 (+) | 381 | WP_187398288.1 | hypothetical protein | - |
| HUG14_RS08210 (HUG14_08210) | - | 1670819..1671328 (+) | 510 | WP_033900119.1 | hypothetical protein | - |
| HUG14_RS08215 (HUG14_08215) | - | 1671321..1672649 (+) | 1329 | WP_033900116.1 | glycosyltransferase | - |
| HUG14_RS08220 (HUG14_08220) | - | 1672876..1673412 (+) | 537 | WP_010905603.1 | HdeD family acid-resistance protein | - |
| HUG14_RS08225 (HUG14_08225) | pnuC | 1673609..1674340 (+) | 732 | WP_187398289.1 | nicotinamide riboside transporter PnuC | - |
| HUG14_RS08230 (HUG14_08230) | - | 1674456..1674611 (-) | 156 | WP_003131383.1 | hypothetical protein | - |
| HUG14_RS08235 (HUG14_08235) | - | 1674869..1675294 (+) | 426 | WP_003131384.1 | universal stress protein | - |
| HUG14_RS08240 (HUG14_08240) | - | 1675416..1675763 (+) | 348 | WP_015426175.1 | HU family DNA-binding protein | - |
Sequence
Protein
Download Length: 144 a.a. Molecular weight: 16204.89 Da Isoelectric Point: 4.7294
>NTDB_id=476663 HUG14_RS08005 WP_187398260.1 1640318..1640752(+) (ssb) [Lactococcus lactis strain K_LL005]
MINNVVLVGRITRDPELRYTPQQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQT
RNYENQQGQRVYITEVVADNFQMLESKGGTKQNEESQNNTAPNFARDGSNDIPGTEISDDDLPF
MINNVVLVGRITRDPELRYTPQQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQT
RNYENQQGQRVYITEVVADNFQMLESKGGTKQNEESQNNTAPNFARDGSNDIPGTEISDDDLPF
Nucleotide
Download Length: 435 bp
>NTDB_id=476663 HUG14_RS08005 WP_187398260.1 1640318..1640752(+) (ssb) [Lactococcus lactis strain K_LL005]
ATGATAAATAATGTCGTATTAGTGGGTCGTATCACACGAGATCCAGAATTAAGATATACACCACAACAAAATCAGGCAGT
TGCAACTTTCAGTTTGGCTGTTAATCGCCAATTTAAGAACGCAAACGGAGAACGTGAAGCAGACTTCATAAATTGCGTAA
TCTGGCGTCAGCAAGCCGAAAATTTAGCAAATTGGGCTAAAAAGGGGGCTTTGATTGGAGTAACAGGTAGAATCCAAACA
AGAAACTATGAAAATCAGCAAGGACAGCGAGTTTATATCACAGAAGTAGTTGCTGACAATTTCCAAATGCTGGAAAGCAA
AGGTGGAACAAAACAAAATGAAGAGTCACAAAACAATACAGCTCCGAATTTTGCCCGTGACGGTTCCAATGATATTCCAG
GCACAGAAATTAGTGATGATGATTTGCCATTTTAG
ATGATAAATAATGTCGTATTAGTGGGTCGTATCACACGAGATCCAGAATTAAGATATACACCACAACAAAATCAGGCAGT
TGCAACTTTCAGTTTGGCTGTTAATCGCCAATTTAAGAACGCAAACGGAGAACGTGAAGCAGACTTCATAAATTGCGTAA
TCTGGCGTCAGCAAGCCGAAAATTTAGCAAATTGGGCTAAAAAGGGGGCTTTGATTGGAGTAACAGGTAGAATCCAAACA
AGAAACTATGAAAATCAGCAAGGACAGCGAGTTTATATCACAGAAGTAGTTGCTGACAATTTCCAAATGCTGGAAAGCAA
AGGTGGAACAAAACAAAATGAAGAGTCACAAAACAATACAGCTCCGAATTTTGCCCGTGACGGTTCCAATGATATTCCAG
GCACAGAAATTAGTGATGATGATTTGCCATTTTAG
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| ssb | Latilactobacillus sakei subsp. sakei 23K |
56 |
100 |
0.681 |
| ssbA | Bacillus subtilis subsp. subtilis str. 168 |
52.542 |
100 |
0.646 |
| ssbB | Bacillus subtilis subsp. subtilis str. 168 |
57.944 |
74.306 |
0.431 |
| ssbB | Streptococcus sobrinus strain NIDR 6715-7 |
42.361 |
100 |
0.424 |
| ssbB/cilA | Streptococcus pneumoniae TIGR4 |
42.361 |
100 |
0.424 |
| ssbB/cilA | Streptococcus mitis NCTC 12261 |
41.667 |
100 |
0.417 |
| ssbB/cilA | Streptococcus pneumoniae Rx1 |
41.667 |
100 |
0.417 |
| ssbB/cilA | Streptococcus pneumoniae D39 |
41.667 |
100 |
0.417 |
| ssbB/cilA | Streptococcus pneumoniae R6 |
41.667 |
100 |
0.417 |
| ssbB/cilA | Streptococcus mitis SK321 |
41.667 |
100 |
0.417 |
| ssbA | Streptococcus mutans UA159 |
38.194 |
100 |
0.382 |