Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HXW73_RS08225 Genome accession   NZ_CP058321
Coordinates   1776324..1777556 (-) Length   410 a.a.
NCBI ID   WP_186255734.1    Uniprot ID   A0A7G7Z851
Organism   Halomonas sp. SH5A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1771324..1782556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXW73_RS08190 (HXW73_08195) - 1771610..1771984 (+) 375 WP_085918486.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -
  HXW73_RS08195 (HXW73_08200) - 1772051..1772881 (-) 831 WP_186255728.1 AraC family transcriptional regulator -
  HXW73_RS08200 (HXW73_08205) - 1773254..1773580 (+) 327 WP_186255729.1 hypothetical protein -
  HXW73_RS08205 (HXW73_08210) - 1773643..1774518 (-) 876 WP_186255730.1 DNA replication terminus site-binding protein -
  HXW73_RS08210 (HXW73_08215) yacG 1774519..1774752 (-) 234 WP_186255731.1 DNA gyrase inhibitor YacG -
  HXW73_RS08215 (HXW73_08220) coaE 1774815..1775417 (-) 603 WP_186255732.1 dephospho-CoA kinase -
  HXW73_RS08220 (HXW73_08225) pilD 1775457..1776302 (-) 846 WP_186255733.1 A24 family peptidase Machinery gene
  HXW73_RS08225 (HXW73_08230) pilC 1776324..1777556 (-) 1233 WP_186255734.1 type II secretion system F family protein Machinery gene
  HXW73_RS08230 (HXW73_08235) pilB 1777560..1779317 (-) 1758 WP_186255735.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HXW73_RS17950 (HXW73_08240) - 1779558..1780025 (+) 468 WP_186255736.1 pilin -
  HXW73_RS08240 (HXW73_08245) - 1780098..1780595 (+) 498 WP_186255737.1 pilin -
  HXW73_RS08245 (HXW73_08250) - 1780727..1781179 (+) 453 WP_240538746.1 anti-virulence regulator CigR family protein -
  HXW73_RS08250 (HXW73_08255) - 1781205..1782023 (+) 819 WP_186255738.1 inositol monophosphatase family protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44897.38 Da        Isoelectric Point: 10.3269

>NTDB_id=463760 HXW73_RS08225 WP_186255734.1 1776324..1777556(-) (pilC) [Halomonas sp. SH5A2]
MAKSPRARRQPSIQLYRWKWEGTGPQNRTLSGEIIGRSKADVTSELANQQINVKRLRKKGGLSGRGRISPHDIMVFARQM
ATMIRAGIPLLQAFQVVAESLKKPAMVGLVQQMMSDVSAGASFSDALRRHPKHFDRLFVNLVEAGEQSGALDEMLDRIAT
YKEKVESLKSRVKKAMWYPSAVLLIGVGVTLLLLIKVVPEFESMFDSFGAELPALTQVTVNLSALAQRYWLYALGGVIAG
VLLLKQAINRSPRVAYRAHSVILRLPVVGDILHKSAVARFSRTLATTFASGVPLVEGLDTASGATGNRVYERAVTQTRHD
VATGQQLHFAMRMTNQFPPLAVQMVSIGEEAGSLDAMLNRVADYYEEEVDNKVDALTSLMEPLIIVVLGLLVGGVVVSMY
LPIFNLGSAL

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=463760 HXW73_RS08225 WP_186255734.1 1776324..1777556(-) (pilC) [Halomonas sp. SH5A2]
ATGGCCAAGTCGCCTCGGGCTCGTCGTCAGCCTAGCATTCAGCTTTACCGCTGGAAGTGGGAAGGCACAGGGCCTCAGAA
TCGCACGCTTAGCGGTGAAATCATTGGTCGTAGCAAGGCAGATGTCACCAGCGAGCTTGCCAATCAGCAGATCAACGTAA
AGCGGTTACGCAAGAAAGGCGGTCTCAGCGGTCGCGGGCGAATCAGCCCACACGACATCATGGTGTTTGCCCGCCAGATG
GCGACCATGATTCGGGCAGGCATTCCGCTACTTCAGGCGTTTCAGGTGGTCGCCGAGAGCCTCAAAAAACCGGCGATGGT
AGGTCTGGTGCAGCAAATGATGAGCGATGTGTCTGCCGGGGCCAGCTTCTCCGATGCACTGCGCCGTCACCCAAAGCACT
TTGACCGCCTGTTTGTGAATCTGGTTGAAGCCGGCGAGCAATCCGGGGCACTTGACGAGATGCTTGATCGCATCGCTACT
TACAAGGAAAAAGTCGAGTCACTTAAAAGCCGCGTCAAGAAGGCCATGTGGTACCCCTCTGCGGTGCTACTGATCGGCGT
CGGGGTCACCCTTCTGCTGCTGATCAAAGTGGTGCCCGAATTCGAAAGCATGTTCGACAGCTTTGGCGCAGAACTGCCCG
CCTTGACGCAGGTGACGGTCAACCTGTCGGCCCTCGCCCAGCGCTATTGGCTGTACGCACTGGGGGGCGTCATCGCGGGG
GTATTGCTGCTCAAGCAGGCAATAAACCGTTCTCCCCGTGTTGCTTACCGCGCACACAGCGTCATATTACGCCTGCCCGT
CGTAGGTGACATTCTGCATAAATCAGCAGTGGCGAGGTTTTCCCGAACCCTGGCCACCACTTTTGCGTCTGGCGTGCCGC
TGGTCGAAGGACTGGATACGGCGTCAGGGGCAACCGGCAATAGGGTTTACGAACGCGCCGTCACCCAGACACGCCATGAC
GTGGCCACCGGTCAGCAGTTACACTTCGCCATGCGCATGACCAATCAGTTCCCGCCCCTGGCGGTGCAAATGGTCAGCAT
CGGTGAAGAAGCAGGCTCATTGGATGCCATGCTAAACCGCGTCGCGGACTACTACGAAGAAGAAGTCGACAACAAGGTGG
ATGCCTTAACCTCGCTGATGGAACCGCTGATTATCGTGGTCTTGGGTCTATTGGTCGGCGGTGTCGTGGTGTCGATGTAC
TTACCCATCTTTAACTTAGGCTCGGCCCTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7Z851

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

51.372

97.805

0.502
  pilC Acinetobacter baylyi ADP1

50.5

97.561

0.493
  pilC Acinetobacter baumannii D1279779

49.37

96.829

0.478
  pilC Legionella pneumophila strain ERS1305867

48.259

98.049

0.473
  pilC Vibrio cholerae strain A1552

43.921

98.293

0.432
  pilC Vibrio campbellii strain DS40M4

43.781

98.049

0.429
  pilG Neisseria gonorrhoeae MS11

42.607

97.317

0.415
  pilG Neisseria meningitidis 44/76-A

42.356

97.317

0.412
  pilC Thermus thermophilus HB27

36.627

100

0.371