Detailed information NTG0463759

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HXW73_RS08220
• Genome accession: NZ_CP058321
• Coordinates: 1775457..1776302 (-)
• Length: 281 a.a.
• NCBI ID: WP_186255733.1
• Uniprot ID: A0A7G7Z850
• Organism: Halomonas sp. SH5A2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1770457..1781302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HXW73_RS08190 (HXW73_08195)	-	1771610..1771984 (+)	375	WP_085918486.1	histone-like nucleoid-structuring protein, MvaT/MvaU family	-
HXW73_RS08195 (HXW73_08200)	-	1772051..1772881 (-)	831	WP_186255728.1	AraC family transcriptional regulator	-
HXW73_RS08200 (HXW73_08205)	-	1773254..1773580 (+)	327	WP_186255729.1	hypothetical protein	-
HXW73_RS08205 (HXW73_08210)	-	1773643..1774518 (-)	876	WP_186255730.1	DNA replication terminus site-binding protein	-
HXW73_RS08210 (HXW73_08215)	yacG	1774519..1774752 (-)	234	WP_186255731.1	DNA gyrase inhibitor YacG	-
HXW73_RS08215 (HXW73_08220)	coaE	1774815..1775417 (-)	603	WP_186255732.1	dephospho-CoA kinase	-
HXW73_RS08220 (HXW73_08225)	pilD	1775457..1776302 (-)	846	WP_186255733.1	A24 family peptidase	Machinery gene
HXW73_RS08225 (HXW73_08230)	pilC	1776324..1777556 (-)	1233	WP_186255734.1	type II secretion system F family protein	Machinery gene
HXW73_RS08230 (HXW73_08235)	pilB	1777560..1779317 (-)	1758	WP_186255735.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HXW73_RS17950 (HXW73_08240)	-	1779558..1780025 (+)	468	WP_186255736.1	pilin	-
HXW73_RS08240 (HXW73_08245)	-	1780098..1780595 (+)	498	WP_186255737.1	pilin	-
HXW73_RS08245 (HXW73_08250)	-	1780727..1781179 (+)	453	WP_240538746.1	anti-virulence regulator CigR family protein	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31179.20 Da
• Isoelectric Point: 8.4690

>NTDB_id=463759 HXW73_RS08220 WP_186255733.1 1775457..1776302(-) (pilD) [Halomonas sp. SH5A2]
MQYFSITFWPLVFLLGLCLGSFLNVVITRLPVMLMRQWRQEARAQLTLDEESSPRFNLAQPRSMCPRCEASIAWHDNLPL
IGWLKRRGRCAHCNARISMQYPLIELAGGILAISIALLYGASWQALWLYGACLTLLALAVIDWRVQLLPDILTLPLLWAG
LIYQWLFQPAFLADAVIGAVVGYMSLWSFYWLFKLTTGKEGMGFGDFKLLAALGAWLGWLSLPLVVLISAGLGAILGLAY
QASSPQARGTPMPFGPFLALAGWIMLLAGNDILAIYLNIVS

Nucleotide

• Length: 846 bp

>NTDB_id=463759 HXW73_RS08220 WP_186255733.1 1775457..1776302(-) (pilD) [Halomonas sp. SH5A2]
ATGCAGTATTTTTCGATCACTTTCTGGCCACTGGTTTTTTTATTGGGGTTATGCCTGGGCAGTTTTCTCAATGTGGTGAT
TACCCGTCTCCCCGTCATGCTGATGCGGCAGTGGCGTCAGGAGGCACGTGCCCAGCTCACACTGGACGAAGAGTCATCGC
CGCGATTCAATCTTGCCCAGCCGCGCTCCATGTGCCCGCGCTGCGAGGCCTCAATCGCCTGGCACGATAACCTGCCGCTG
ATTGGCTGGCTCAAACGACGTGGGCGCTGCGCGCACTGCAATGCTCGGATCAGCATGCAATATCCGCTCATCGAGCTTGC
GGGTGGCATCCTGGCTATCAGTATCGCCCTGCTTTATGGCGCCAGTTGGCAGGCGCTATGGCTTTATGGCGCCTGCTTGA
CGCTGCTTGCCCTGGCCGTCATTGATTGGCGTGTACAGCTACTGCCCGATATTTTGACACTGCCGCTGCTGTGGGCCGGT
TTGATCTATCAATGGCTTTTCCAGCCTGCTTTCCTGGCCGATGCCGTTATCGGCGCGGTGGTGGGGTATATGAGCCTCTG
GAGTTTTTACTGGCTGTTCAAGCTCACTACCGGCAAGGAAGGCATGGGCTTTGGCGACTTCAAGCTGCTCGCTGCATTAG
GCGCCTGGCTAGGTTGGCTATCGCTTCCGCTGGTCGTGCTGATTTCAGCCGGTTTGGGCGCCATACTCGGCCTGGCCTAT
CAGGCGTCATCACCCCAGGCGCGCGGCACCCCCATGCCGTTCGGGCCTTTTTTAGCGCTCGCCGGGTGGATAATGCTGCT
CGCTGGCAACGACATATTAGCGATATACCTCAATATCGTCAGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (132-238)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-117)

3D structure

• AlphaFold DB: A0A7G7Z850

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.104	99.288	0.488
pilD	Vibrio campbellii strain DS40M4	45.552	100	0.456
pilD	Acinetobacter baumannii D1279779	45.185	96.085	0.434
pilD	Acinetobacter nosocomialis M2	45.185	96.085	0.434
pilD	Neisseria gonorrhoeae MS11	45.318	95.018	0.431