Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HXW73_RS08230 Genome accession   NZ_CP058321
Coordinates   1777560..1779317 (-) Length   585 a.a.
NCBI ID   WP_186255735.1    Uniprot ID   A0A7G7Z852
Organism   Halomonas sp. SH5A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1772560..1784317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXW73_RS08200 (HXW73_08205) - 1773254..1773580 (+) 327 WP_186255729.1 hypothetical protein -
  HXW73_RS08205 (HXW73_08210) - 1773643..1774518 (-) 876 WP_186255730.1 DNA replication terminus site-binding protein -
  HXW73_RS08210 (HXW73_08215) yacG 1774519..1774752 (-) 234 WP_186255731.1 DNA gyrase inhibitor YacG -
  HXW73_RS08215 (HXW73_08220) coaE 1774815..1775417 (-) 603 WP_186255732.1 dephospho-CoA kinase -
  HXW73_RS08220 (HXW73_08225) pilD 1775457..1776302 (-) 846 WP_186255733.1 A24 family peptidase Machinery gene
  HXW73_RS08225 (HXW73_08230) pilC 1776324..1777556 (-) 1233 WP_186255734.1 type II secretion system F family protein Machinery gene
  HXW73_RS08230 (HXW73_08235) pilB 1777560..1779317 (-) 1758 WP_186255735.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HXW73_RS17950 (HXW73_08240) - 1779558..1780025 (+) 468 WP_186255736.1 pilin -
  HXW73_RS08240 (HXW73_08245) - 1780098..1780595 (+) 498 WP_186255737.1 pilin -
  HXW73_RS08245 (HXW73_08250) - 1780727..1781179 (+) 453 WP_240538746.1 anti-virulence regulator CigR family protein -
  HXW73_RS08250 (HXW73_08255) - 1781205..1782023 (+) 819 WP_186255738.1 inositol monophosphatase family protein -
  HXW73_RS08260 (HXW73_08265) - 1782429..1782581 (+) 153 WP_186255739.1 hypothetical protein -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 64112.37 Da        Isoelectric Point: 5.9694

>NTDB_id=463761 HXW73_RS08230 WP_186255735.1 1777560..1779317(-) (pilB) [Halomonas sp. SH5A2]
MSEPNFESTLSHAAARGGLRGIAQRLVKHGLLTEAQAATAEATAFDLDISLLQHVVDSGLVDPRQSAIAAGWEYGLPVID
LNAVRLSSLPPAAEYPVKVLQNLTVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSVEGVLAPVDQLSTALNQYLTQN
ERSMLEELDDIDDAVSSLNVVQGAAGEDAPLEEAPQNSDDAPIVKFVNKLLLDAIRRGASDIHFEPYETQYRVRFRIDGM
LIEVARPPFAMRNRIAARLKIMARLDISERRLPQDGAIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTATQLGIEQL
GFTDDQRGYYERALAQPQGMILVTGPTGSGKTVSLYTGINILNQIERNICTAEDPVEIKVPGVNQVNVLPKIGLDFASAL
RAFLRQDPDVVMVGEIRDLETAEISIKAAQTGHLVLSTVHTNSAAETLTRLSNMGVSSFNIASAVSLIIAQRLARKLCPH
CKEPADIPHEALRREGFSQQEIQQATLFRPVGCKQCTHGYKGRVGVYEVVPITDAMRQLVMRDANSLDIDQLARREGYPS
LHQSGLLKVMQGITSLEEVNRISKE

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=463761 HXW73_RS08230 WP_186255735.1 1777560..1779317(-) (pilB) [Halomonas sp. SH5A2]
ATGAGCGAACCCAATTTCGAGTCCACTTTAAGTCATGCCGCCGCCCGGGGCGGGCTGCGCGGCATCGCCCAGCGTCTGGT
CAAGCACGGGTTACTGACCGAGGCTCAGGCGGCCACCGCTGAGGCGACGGCGTTCGACTTGGATATTTCACTGCTCCAAC
ACGTCGTTGATAGCGGCCTGGTGGACCCTAGGCAATCAGCCATTGCCGCTGGCTGGGAGTACGGGTTGCCGGTGATTGAT
CTCAATGCCGTGCGGTTATCGTCACTGCCGCCAGCGGCCGAGTACCCCGTTAAAGTCCTTCAGAATTTGACGGTGCTGCC
ATTGGCGCGTCACGGCCACCGGCTTACCGTCGCCGTCCCCTACCCGGCGACGCTCACCCAGCTGGATGAGCTGCAATTTG
CCACCGGGCTGAGCGTTGAAGGGGTGCTAGCGCCTGTCGATCAACTGAGTACGGCGTTAAACCAGTATCTCACCCAGAAT
GAGCGCAGCATGCTGGAGGAGCTTGATGACATCGACGATGCCGTCAGCTCCTTGAATGTTGTCCAGGGGGCGGCCGGTGA
AGACGCTCCCCTTGAGGAAGCACCGCAGAACAGCGACGACGCACCGATCGTCAAATTCGTCAATAAACTGTTACTGGATG
CGATTCGCCGGGGCGCCTCGGATATCCATTTTGAGCCCTATGAAACCCAGTACCGGGTTCGCTTTCGGATTGATGGCATG
CTGATCGAGGTGGCCAGGCCACCTTTCGCCATGCGTAACCGGATCGCCGCACGTCTGAAGATCATGGCCCGGCTGGATAT
CTCTGAGCGCCGTCTGCCCCAGGACGGGGCGATCAAACTCAAGCTTTCACGTACTCGCTCCCTCGACTTCAGGGTCAACT
CGCTGCCCACCGTGTATGGCGAAAAACTCGTACTGAGGATCCTTGATCCCACCGCCACGCAATTAGGCATCGAGCAGTTA
GGCTTCACCGATGACCAACGCGGTTACTACGAACGGGCGCTGGCGCAGCCACAAGGCATGATTCTGGTGACCGGCCCGAC
AGGCAGCGGTAAAACCGTATCGCTGTATACCGGCATCAATATCCTTAATCAGATAGAGCGCAATATCTGCACCGCCGAAG
ACCCAGTGGAAATCAAAGTCCCCGGCGTTAACCAGGTCAACGTACTGCCGAAAATCGGCCTGGATTTCGCCAGCGCTTTA
AGGGCGTTCCTGCGCCAGGATCCTGACGTGGTCATGGTCGGTGAAATCCGCGACCTGGAAACCGCCGAGATCTCCATCAA
AGCGGCCCAGACCGGCCACCTTGTGCTGTCGACGGTACATACCAACTCGGCGGCCGAAACCCTCACTCGTCTATCCAATA
TGGGGGTGTCTTCCTTCAATATTGCCAGCGCGGTCAGTTTGATCATTGCCCAGCGCCTGGCGCGCAAGCTATGTCCTCAC
TGCAAGGAACCGGCTGATATTCCCCACGAAGCACTGAGACGCGAAGGGTTCAGCCAACAGGAGATTCAGCAGGCCACCCT
ATTTCGTCCGGTCGGTTGCAAGCAGTGTACCCATGGCTACAAGGGCCGTGTGGGTGTCTACGAAGTCGTGCCGATTACCG
ATGCGATGCGCCAGTTGGTCATGCGCGATGCCAACTCTCTGGATATTGACCAACTGGCCCGCCGTGAGGGCTATCCGAGC
TTGCATCAAAGTGGTCTTTTAAAGGTGATGCAAGGGATCACGAGCCTTGAGGAAGTTAACCGCATCAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-159)

Type II/IV secretion system protein (T2SSE)

(209-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7Z852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.18

96.752

0.515
  pilB Acinetobacter baumannii D1279779

51.323

96.923

0.497
  pilB Acinetobacter baylyi ADP1

50

98.12

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.201

96.239

0.474
  pilB Vibrio campbellii strain DS40M4

48.592

97.094

0.472
  pilB Vibrio cholerae strain A1552

50.98

87.179

0.444
  pilF Neisseria gonorrhoeae MS11

50

81.709

0.409