Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HV559_RS06195 Genome accession   NZ_CP055306
Coordinates   1277647..1279047 (+) Length   466 a.a.
NCBI ID   WP_176809846.1    Uniprot ID   A0A7D5IAD3
Organism   Mannheimia pernigra strain BNO311     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1272647..1284047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV559_RS06165 (HV559_06165) - 1272758..1273522 (-) 765 WP_176808291.1 TSUP family transporter -
  HV559_RS06170 (HV559_06170) proB 1273522..1274613 (-) 1092 WP_176809844.1 glutamate 5-kinase -
  HV559_RS06175 (HV559_06175) - 1274728..1275222 (+) 495 WP_159629590.1 dihydrofolate reductase -
  HV559_RS06180 (HV559_06180) - 1275350..1275583 (+) 234 WP_159629593.1 YgjV family protein -
  HV559_RS06185 (HV559_06185) radA 1275622..1277001 (-) 1380 WP_176808292.1 DNA repair protein RadA Machinery gene
  HV559_RS06190 (HV559_06190) pilA 1277182..1277625 (+) 444 WP_176809845.1 prepilin peptidase-dependent pilin Machinery gene
  HV559_RS06195 (HV559_06195) pilB 1277647..1279047 (+) 1401 WP_176809846.1 GspE/PulE family protein Machinery gene
  HV559_RS06200 (HV559_06200) pilC 1279031..1280224 (+) 1194 WP_176809847.1 type II secretion system F family protein Machinery gene
  HV559_RS06205 (HV559_06205) - 1280227..1280883 (+) 657 WP_176808296.1 prepilin peptidase -
  HV559_RS06210 (HV559_06210) coaE 1280915..1281553 (+) 639 WP_176809848.1 dephospho-CoA kinase -
  HV559_RS06215 (HV559_06215) yacG 1281535..1281720 (+) 186 WP_159629615.1 DNA gyrase inhibitor YacG -
  HV559_RS06220 (HV559_06220) holA 1281721..1282743 (-) 1023 WP_176809849.1 DNA polymerase III subunit delta -
  HV559_RS06225 (HV559_06225) lptE 1282784..1283272 (-) 489 WP_176808298.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 52717.48 Da        Isoelectric Point: 5.8300

>NTDB_id=457530 HV559_RS06195 WP_176809846.1 1277647..1279047(+) (pilB) [Mannheimia pernigra strain BNO311]
MAYSVTDLNSQQIIEISAEQWAKNGNEKQILLRYLAVPLRETESQLWLAIDDMQNLSACEIFAFIHHKQIEPVLISSDEL
KFLLNALSPEQSRLQSEIYEDNAAYSLSNAEQVDRNDPIIQILDNLFKFCLKNNASDIHLEPQKERLLIRLRIDGVLHLY
KTFPQNVSNRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRIQKNKPINLDFLELGFTP
SQKEKLLNALNQPQGLILVTGPTGSGKSITLYSALSHLNSNEKHILTAEDPIEIEIEGIIQTQVNRGIHLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELKNALLLVVAQRLLRRTCTKCGGI
GCENCYQGYKGRIGIYQLLSRTAKNFEKTTACLDFETLAESAKEKLVEGKTDQNEILRVLGNGENL

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=457530 HV559_RS06195 WP_176809846.1 1277647..1279047(+) (pilB) [Mannheimia pernigra strain BNO311]
ATGGCGTATTCAGTTACCGATCTTAACAGCCAGCAAATCATTGAAATTTCTGCCGAACAATGGGCAAAAAACGGCAATGA
AAAGCAGATTTTGTTGCGATATTTAGCCGTTCCGTTGCGAGAAACTGAATCACAATTATGGTTAGCGATTGATGATATGC
AAAACTTGAGTGCGTGTGAAATCTTTGCTTTTATTCATCATAAGCAGATTGAGCCTGTTTTGATTTCCTCTGATGAGTTG
AAATTTTTACTTAATGCCCTTTCACCCGAGCAGAGTCGCTTGCAGAGTGAAATTTATGAGGATAATGCCGCTTATTCGTT
ATCAAATGCAGAGCAGGTAGATAGAAACGATCCGATTATTCAAATTTTAGATAATCTCTTTAAATTTTGCTTAAAAAATA
ATGCCTCAGACATCCATTTGGAGCCTCAGAAAGAGAGGTTACTCATTCGGCTAAGAATTGATGGAGTTTTACATCTTTAT
AAAACGTTTCCTCAGAATGTATCAAACCGACTGATTTCTCGAATTAAATTACTAGCGAAACTAGATATTAGTGAAACCCG
TTTGCCTCAAGATGGGCAATTTCATTTTAAAACGATTTTAGCAGAAACCTTAGATTTCCGCGTTTCAACTTTGCCTACGC
ATTTTGGTGAAAAAGTGGTACTTCGGATTCAAAAAAATAAGCCAATTAATTTGGACTTTTTAGAGTTAGGATTTACCCCA
AGCCAGAAAGAAAAATTGCTCAATGCACTGAATCAACCGCAAGGCTTGATTCTAGTAACAGGGCCGACAGGTAGTGGTAA
AAGTATCACGCTTTATAGTGCATTAAGTCATTTAAATAGCAATGAAAAACATATTTTAACTGCGGAAGATCCAATTGAAA
TTGAGATAGAGGGTATTATTCAAACTCAGGTAAATCGTGGTATTCATCTTGATTTTAGCCAACTACTGAGAACCTTCCTT
CGACAAGATCCTGACATCATTATGCTAGGTGAGATCCGTGATGAGGAGAGTGCAGAAATTGCCTTAAGAGCGGCACAAAC
TGGGCATTTGGTTCTTTCGACATTACATACTAATGATGCACCCTCTGCCATAGAGCGATTATTACAACTCGGGATAAAAG
AATACGAGCTGAAAAATGCGTTGTTGCTTGTGGTGGCTCAACGTTTATTAAGGCGAACTTGCACAAAATGTGGCGGTATC
GGTTGCGAAAATTGCTATCAAGGCTACAAAGGTAGAATTGGGATTTATCAACTCTTGAGCAGAACTGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTAGATTTTGAAACCTTAGCTGAGAGTGCAAAAGAAAAATTAGTGGAAGGAAAAACAGATCAGA
ATGAAATTTTGAGGGTATTAGGAAATGGCGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)

Type II/IV secretion system protein (T2SSE)

(131-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5IAD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.435

98.712

0.695
  pilB Haemophilus influenzae 86-028NP

57.269

97.425

0.558
  pilB Haemophilus influenzae Rd KW20

56.828

97.425

0.554
  pilB Vibrio campbellii strain DS40M4

41.079

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.956

100

0.423
  pilB Vibrio cholerae strain A1552

39.834

100

0.412
  pilB Legionella pneumophila strain ERS1305867

39.22

100

0.41
  pilB Acinetobacter baylyi ADP1

37.295

100

0.391
  pilB Acinetobacter baumannii D1279779

36.81

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.712

100

0.382
  ctsE Campylobacter jejuni subsp. jejuni 81-176

42.446

89.485

0.38
  pilF Thermus thermophilus HB27

35.805

100

0.363