Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HV559_RS06190 Genome accession   NZ_CP055306
Coordinates   1277182..1277625 (+) Length   147 a.a.
NCBI ID   WP_176809845.1    Uniprot ID   A0A7D5HSU3
Organism   Mannheimia pernigra strain BNO311     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1272182..1282625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV559_RS06165 (HV559_06165) - 1272758..1273522 (-) 765 WP_176808291.1 TSUP family transporter -
  HV559_RS06170 (HV559_06170) proB 1273522..1274613 (-) 1092 WP_176809844.1 glutamate 5-kinase -
  HV559_RS06175 (HV559_06175) - 1274728..1275222 (+) 495 WP_159629590.1 dihydrofolate reductase -
  HV559_RS06180 (HV559_06180) - 1275350..1275583 (+) 234 WP_159629593.1 YgjV family protein -
  HV559_RS06185 (HV559_06185) radA 1275622..1277001 (-) 1380 WP_176808292.1 DNA repair protein RadA Machinery gene
  HV559_RS06190 (HV559_06190) pilA 1277182..1277625 (+) 444 WP_176809845.1 prepilin peptidase-dependent pilin Machinery gene
  HV559_RS06195 (HV559_06195) pilB 1277647..1279047 (+) 1401 WP_176809846.1 GspE/PulE family protein Machinery gene
  HV559_RS06200 (HV559_06200) pilC 1279031..1280224 (+) 1194 WP_176809847.1 type II secretion system F family protein Machinery gene
  HV559_RS06205 (HV559_06205) - 1280227..1280883 (+) 657 WP_176808296.1 prepilin peptidase -
  HV559_RS06210 (HV559_06210) coaE 1280915..1281553 (+) 639 WP_176809848.1 dephospho-CoA kinase -
  HV559_RS06215 (HV559_06215) yacG 1281535..1281720 (+) 186 WP_159629615.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15847.22 Da        Isoelectric Point: 9.0670

>NTDB_id=457529 HV559_RS06190 WP_176809845.1 1277182..1277625(+) (pilA) [Mannheimia pernigra strain BNO311]
MQKMYLFRPLAKAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLRASASYKSDVELCIYNRNGLDNCSGGTNGI
QANKSDTSDTKYLKTITVNKGVISVTGKGSLDGYSYTLTPAFSNNTISWTASCKGKDTSLFPAGFCN

Nucleotide


Download         Length: 444 bp        

>NTDB_id=457529 HV559_RS06190 WP_176809845.1 1277182..1277625(+) (pilA) [Mannheimia pernigra strain BNO311]
ATGCAAAAAATGTATCTTTTTCGACCGCTTGCAAAAGCGTTTACCTTGATTGAGCTAATGATCGTGATTGCAATTATTGC
CATTCTAGCCACTATTGCGATTCCATCTTACAACTCTTACACACAAAAAGCGGCTTTATCTGAATTATTGAGAGCATCGG
CTTCTTATAAATCAGATGTTGAGCTGTGTATTTATAACCGTAATGGGCTGGATAATTGTTCGGGCGGTACAAATGGTATT
CAAGCCAATAAATCTGATACTAGCGACACCAAATATCTTAAGACGATTACGGTAAATAAAGGCGTGATTAGCGTAACAGG
AAAAGGCTCGCTTGACGGCTATAGCTACACGCTAACACCAGCCTTTTCCAATAATACGATCAGTTGGACTGCAAGTTGTA
AGGGTAAAGATACGAGTCTGTTCCCCGCAGGTTTTTGTAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5HSU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

70.946

100

0.714
  pilA Haemophilus influenzae 86-028NP

56.552

98.639

0.558
  pilA Haemophilus influenzae Rd KW20

54.11

99.32

0.537
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

32.335

100

0.367