Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HUW52_RS06075 Genome accession   NZ_CP054880
Coordinates   1370588..1371805 (-) Length   405 a.a.
NCBI ID   WP_175553183.1    Uniprot ID   -
Organism   Pseudomonas sp. 43A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1365588..1376805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW52_RS06035 (HUW52_06035) - 1366427..1366600 (-) 174 WP_007909875.1 DUF3094 domain-containing protein -
  HUW52_RS06040 (HUW52_06040) - 1366652..1367179 (-) 528 WP_175553179.1 MOSC domain-containing protein -
  HUW52_RS06045 (HUW52_06045) - 1367176..1367802 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  HUW52_RS06050 (HUW52_06050) - 1367920..1368609 (+) 690 WP_175553180.1 energy-coupling factor ABC transporter permease -
  HUW52_RS06055 (HUW52_06055) - 1368675..1368890 (+) 216 WP_008078491.1 hypothetical protein -
  HUW52_RS06060 (HUW52_06060) yacG 1368897..1369097 (-) 201 WP_007959585.1 DNA gyrase inhibitor YacG -
  HUW52_RS06065 (HUW52_06065) coaE 1369094..1369717 (-) 624 WP_175553181.1 dephospho-CoA kinase -
  HUW52_RS06070 (HUW52_06070) pilD 1369714..1370586 (-) 873 WP_175553182.1 A24 family peptidase Machinery gene
  HUW52_RS06075 (HUW52_06075) pilC 1370588..1371805 (-) 1218 WP_175553183.1 type II secretion system F family protein Machinery gene
  HUW52_RS06080 (HUW52_06080) pilB 1371808..1373508 (-) 1701 WP_175553184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUW52_RS06085 (HUW52_06085) - 1373735..1374166 (+) 432 WP_175553185.1 pilin -
  HUW52_RS06090 (HUW52_06090) - 1374464..1374820 (+) 357 WP_016986244.1 BON domain-containing protein -
  HUW52_RS06095 (HUW52_06095) - 1375106..1375402 (-) 297 WP_016986246.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44080.78 Da        Isoelectric Point: 10.1093

>NTDB_id=455256 HUW52_RS06075 WP_175553183.1 1370588..1371805(-) (pilC) [Pseudomonas sp. 43A]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ERLKAKIKKAMTYPSAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGALVAAIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDEMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=455256 HUW52_RS06075 WP_175553183.1 1370588..1371805(-) (pilC) [Pseudomonas sp. 43A]
ATGGCGGTCAAGGCAGCAAAAATCAGTATCTACGCCTGGGAAGGTACGGACAGGAAAGGCAGCAAAGTCACCGGTGAGCT
GAGCGGACAAAACCCGGCGCTGATCAAGGCACAACTGCGTAAACAGGGGATCAATCCCGGCAAGGTGCGCAAAAAATCCG
CGTCGTTGTTGAGTTTTGGCAAACGCATCAAGGCCCAGGACATCGCTCTGTTCACCCGGCAAATGGCGACCATGATGAAG
GCAGGTGTGCCGCTGCTGCAGTCGTTCGACATCATTGGCGAAGGCTTTGAAAACCCGGCCATGCGCAAGCTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTTGCCACAGCCCTGCGCAAGAAGCCGCAGTATTTCGACGAGTTGTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCCGGCGCGCTCGACACCTTGCTCGAACGGGTCGCGACTTATAAGGAAAAAAGC
GAACGGCTCAAGGCCAAGATCAAGAAAGCCATGACTTACCCCTCCGCCGTGGTGCTGGTCGCAGCGGTTGTGACCGGAAT
TCTGCTGGTTAAAGTGGTGCCGCAGTTTCAGTCGGTTTTTTCCGGTTTCGGTGCTGAGCTGCCGGCCTTCACGCTGATGG
TGATCAGCCTTTCGGAATTCATGCAGCAATGGTGGTGGGCGATTCTTGGCGCGCTGGTCGCGGCGATTTTCGGCACTCGT
CACGCACTGAAAACCTCCCAGGCGCTACGTGATCGCAAAGACACCTGGCTGTTGAAACTACCCTTGGTCGGCACGCTGAT
GTACAAGTCAGCGGTCGCCCGTTTTGCCCGAACCCTGTCGACCACGTTTGCTGCCGGTGTGCCATTGGTGGAAGCGCTCG
ACTCGGTGGCCGGCGCCACAGGCAACGTTGTGTTCAAGCGCGCGGTGCTGCGCATTCGCCAGGACGTCTCCACCGGCATG
CAGCTGAATTTCTCGATGCGCAGCACCGGGGTCTTCCCCAACATGGCCGTGCAGATGACGGCGATTGGCGAAGAGTCCGG
CGCGCTGGACGAAATGCTCGACAAGGTCGCGGGGTTTTACGAGGAGGAAGTGGATAACATGGTCGACAATCTTACCAGCC
TTATGGAGCCATTCATCATGGTCGTGCTGGGGGTAATCGTCGGCGGGCTGGTGGTGGCCATGTATCTGCCGATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

38.903

99.012

0.385