Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUW52_RS06080 Genome accession   NZ_CP054880
Coordinates   1371808..1373508 (-) Length   566 a.a.
NCBI ID   WP_175553184.1    Uniprot ID   -
Organism   Pseudomonas sp. 43A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1366808..1378508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW52_RS06045 (HUW52_06045) - 1367176..1367802 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  HUW52_RS06050 (HUW52_06050) - 1367920..1368609 (+) 690 WP_175553180.1 energy-coupling factor ABC transporter permease -
  HUW52_RS06055 (HUW52_06055) - 1368675..1368890 (+) 216 WP_008078491.1 hypothetical protein -
  HUW52_RS06060 (HUW52_06060) yacG 1368897..1369097 (-) 201 WP_007959585.1 DNA gyrase inhibitor YacG -
  HUW52_RS06065 (HUW52_06065) coaE 1369094..1369717 (-) 624 WP_175553181.1 dephospho-CoA kinase -
  HUW52_RS06070 (HUW52_06070) pilD 1369714..1370586 (-) 873 WP_175553182.1 A24 family peptidase Machinery gene
  HUW52_RS06075 (HUW52_06075) pilC 1370588..1371805 (-) 1218 WP_175553183.1 type II secretion system F family protein Machinery gene
  HUW52_RS06080 (HUW52_06080) pilB 1371808..1373508 (-) 1701 WP_175553184.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUW52_RS06085 (HUW52_06085) - 1373735..1374166 (+) 432 WP_175553185.1 pilin -
  HUW52_RS06090 (HUW52_06090) - 1374464..1374820 (+) 357 WP_016986244.1 BON domain-containing protein -
  HUW52_RS06095 (HUW52_06095) - 1375106..1375402 (-) 297 WP_016986246.1 DUF2845 domain-containing protein -
  HUW52_RS06100 (HUW52_06100) - 1375402..1378353 (-) 2952 WP_175553186.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62779.84 Da        Isoelectric Point: 6.0463

>NTDB_id=455257 HUW52_RS06080 WP_175553184.1 1371808..1373508(-) (pilB) [Pseudomonas sp. 43A]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
LDVESIDDSKQDTIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPVQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=455257 HUW52_RS06080 WP_175553184.1 1371808..1373508(-) (pilB) [Pseudomonas sp. 43A]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTGACAGAAAAGAGTGCCCAGCAAGC
CTGGCAACAGGCTCAGCGTAATCGCTTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTGGCAGGTCG
CAGAGATCGCCTCCGAGCACTTCGGCATGGCCTTCCTTGATCTCAATTGCCTGGACAAAGAAACGCAGCCCAAAGGGCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGGCGCGGCAATAAGCTGTTCGTGGGGATTTCCGA
CCCGAGCAACCATCAAGCGATCAACGACATCCAGTTCAGCACTGGGCTAAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAGAAATTCTTCGACACCCACGCCAGTGGCCTGGAAGACATGGCCGATGTCGATCTTGACGGC
CTGGATGTCGAATCCATCGATGACAGCAAGCAAGACACGATTGGCGGACTCGACGCCGACGATGCGCCGGTGGTGCGTTT
TGTTCACAAGATGTTGCTCGACGCGATCAAAAGCGGTTCTTCCGATTTGCACTTCGAGCCCTACGAGAAGAACTACCGGG
TGCGAGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAACCGCCGATTCAACTGGCCGGGCGGATCGCTGCACGTTTG
AAGGTCATGGCCAGTCTCGATATTTCGGAGCGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCAACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CACAGATCGGCATCGACGCGCTCGGCTACGAGCCGGTGCAGAAAGACTTGTACATGGCCGCGCTCAAGCAGCCTCAAGGC
ATGATTCTGGTCACTGGCCCCACCGGCTCGGGGAAAACCGTGTCGCTGTACACCGGCCTGAATATCCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAGG
GCCTCGACTTTGCCCAGGCGCTGCGCTCGTTTCTACGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAAGCCGCGCAGACTGGTCATCTGGTGCTCTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATCCCCGGCTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCTCAGCGCC
TGGCGCGAAAGCTGTGCAGCCACTGCAAGAAGCCCATCGAGATCCCACGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGTTGCAACGCTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACATTCAAA
TGCGCCGCGACGGCTTCGACGACTTGCGCACCTCAGGGCTGCACAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.407

99.823

0.583
  pilB Acinetobacter baumannii D1279779

58.156

99.647

0.58
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilB Vibrio campbellii strain DS40M4

48.611

100

0.495
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilF Thermus thermophilus HB27

36.918

98.587

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362