Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HUO05_RS11310 Genome accession   NZ_CP054700
Coordinates   2422147..2423163 (-) Length   338 a.a.
NCBI ID   WP_017633531.1    Uniprot ID   A0AAW9AS90
Organism   Vibrio alginolyticus strain GS_MYPK1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2417147..2428163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUO05_RS11285 (HUO05_11285) aroK 2418020..2418538 (-) 519 WP_005381319.1 shikimate kinase AroK -
  HUO05_RS11290 (HUO05_11290) pilQ 2418726..2420471 (-) 1746 WP_165439071.1 type IV pilus secretin PilQ Machinery gene
  HUO05_RS11295 (HUO05_11295) pilP 2420497..2421012 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  HUO05_RS11300 (HUO05_11300) pilO 2421002..2421595 (-) 594 WP_005381322.1 type 4a pilus biogenesis protein PilO Machinery gene
  HUO05_RS11305 (HUO05_11305) pilN 2421588..2422163 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  HUO05_RS11310 (HUO05_11310) pilM 2422147..2423163 (-) 1017 WP_017633531.1 type IV pilus biogenesis protein PilM Machinery gene
  HUO05_RS11315 (HUO05_11315) - 2423308..2425869 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -
  HUO05_RS11320 (HUO05_11320) oxyR 2425923..2426834 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  HUO05_RS11325 (HUO05_11325) - 2427004..2427732 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37609.97 Da        Isoelectric Point: 7.2224

>NTDB_id=454033 HUO05_RS11310 WP_017633531.1 2422147..2423163(-) (pilM) [Vibrio alginolyticus strain GS_MYPK1]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLVEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=454033 HUO05_RS11310 WP_017633531.1 2422147..2423163(-) (pilM) [Vibrio alginolyticus strain GS_MYPK1]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAGGGGGCCTCCACTCCCATGCTCGTAGAAGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503