Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   I6G27_RS05295 Genome accession   NZ_CP065751
Coordinates   1186950..1187453 (-) Length   167 a.a.
NCBI ID   WP_156627541.1    Uniprot ID   A0A378Q862
Organism   Moraxella osloensis strain FDAARGOS_870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1181950..1192453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G27_RS10970 (I6G27_05280) - 1184371..1184931 (+) 561 WP_269465434.1 type IV pilin protein -
  I6G27_RS05285 (I6G27_05285) pilE 1184931..1185347 (+) 417 WP_062331500.1 type IV pilin protein Machinery gene
  I6G27_RS05290 (I6G27_05290) - 1185673..1186950 (-) 1278 WP_062331497.1 O-antigen ligase -
  I6G27_RS05295 (I6G27_05295) pilA2 1186950..1187453 (-) 504 WP_156627541.1 pilin Machinery gene
  I6G27_RS05300 (I6G27_05300) - 1187923..1189722 (+) 1800 WP_062331493.1 PglL family O-oligosaccharyltransferase -
  I6G27_RS05305 (I6G27_05305) - 1189722..1190987 (+) 1266 WP_062331490.1 exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase -
  I6G27_RS05310 (I6G27_05310) pseB 1191027..1192025 (+) 999 WP_062331488.1 UDP-N-acetylglucosamine 4,6-dehydratase (inverting) -

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 16668.13 Da        Isoelectric Point: 8.1079

>NTDB_id=451100 I6G27_RS05295 WP_156627541.1 1186950..1187453(-) (pilA2) [Moraxella osloensis strain FDAARGOS_870]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAKISEGLTLSNGLKTAIAESFQSKGPSSMACTDATTCASIGASP
MDATALAGNKNVASITSDAAGVITIVYKPAVVPTGSNNLTLTPVGADGTTALNLSAAASAGSQVNWRCGGTGTTVAAKFL
PANCRGT

Nucleotide


Download         Length: 504 bp        

>NTDB_id=451100 I6G27_RS05295 WP_156627541.1 1186950..1187453(-) (pilA2) [Moraxella osloensis strain FDAARGOS_870]
ATGAACGCTCAAAAAGGTTTTACCCTTATCGAATTAATGATCGTTGTCGCTATTATCGGTATTTTGGCTGCGATTGCGAT
TCCTGCTTATCAGGATTATACAATTCGAGCTAAGATTTCTGAAGGCTTGACTTTATCAAATGGTTTAAAAACGGCTATTG
CTGAAAGTTTCCAAAGTAAAGGGCCTTCTAGCATGGCATGTACTGATGCAACTACATGTGCATCAATAGGAGCATCTCCA
ATGGATGCGACAGCTTTAGCGGGTAATAAAAATGTTGCTTCTATCACAAGTGATGCGGCTGGTGTAATTACAATTGTATA
TAAGCCAGCGGTTGTTCCTACTGGTTCAAATAACTTAACACTTACGCCAGTTGGTGCAGATGGTACTACAGCATTAAATT
TAAGTGCTGCAGCAAGTGCGGGTTCTCAAGTAAATTGGCGGTGTGGTGGAACTGGTACAACAGTTGCTGCAAAATTTTTA
CCTGCAAACTGCCGTGGGACCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378Q862

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

47.561

98.204

0.467
  pilA2 Legionella pneumophila strain ERS1305867

46.951

98.204

0.461
  pilA Ralstonia pseudosolanacearum GMI1000

41.899

100

0.449
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.895

100

0.431
  comP Acinetobacter baylyi ADP1

41.279

100

0.425
  pilE Neisseria gonorrhoeae strain FA1090

38.068

100

0.401
  pilA Pseudomonas aeruginosa PAK

37.278

100

0.377
  pilE Neisseria gonorrhoeae MS11

35.795

100

0.377
  pilA/pilA1 Eikenella corrodens VA1

36.905

100

0.371