Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPY08_RS02330 Genome accession   NZ_CP053808
Coordinates   485679..487367 (-) Length   562 a.a.
NCBI ID   WP_001960917.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480679..492367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY08_RS02305 (HPY08_02310) yacG 481745..481942 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY08_RS02310 (HPY08_02315) zapD 482100..482840 (-) 741 WP_000207194.1 cell division protein ZapD -
  HPY08_RS02315 (HPY08_02320) coaE 482872..483480 (-) 609 WP_162797106.1 dephospho-CoA kinase -
  HPY08_RS02320 (HPY08_02325) pilD 483477..484352 (-) 876 WP_114716880.1 A24 family peptidase Machinery gene
  HPY08_RS02325 (HPY08_02330) pilC 484410..485636 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY08_RS02330 (HPY08_02335) pilB 485679..487367 (-) 1689 WP_001960917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY08_RS02335 (HPY08_02340) pilA 487374..487850 (-) 477 WP_095473243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY08_RS02340 (HPY08_02345) nadC 488095..488985 (-) 891 WP_114716881.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY08_RS02345 (HPY08_02350) ampD 489283..489828 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY08_RS02350 (HPY08_02355) fldB 489998..490516 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY08_RS02355 (HPY08_02360) xerD 490654..491562 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY08_RS02360 (HPY08_02365) dsbC 491579..492331 (+) 753 WP_071179237.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62057.16 Da        Isoelectric Point: 6.0856

>NTDB_id=447645 HPY08_RS02330 WP_001960917.1 485679..487367(-) (pilB) [Vibrio cholerae strain SP7G]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=447645 HPY08_RS02330 WP_001960917.1 485679..487367(-) (pilB) [Vibrio cholerae strain SP7G]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAATTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGAACTTCAGTGCCCGAGGCGTTGCTTGGTTTAGGGATTTTTCACCCCCAAGAACTCACCGAACAACTGA
GCCATATTTTCGGTTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCTCTATGCCAACAGCTCGGCCTGCGTGAA
CTGATAACCCGCTACGATGCCTTACCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCGGTATCTGACCCCACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCGACTGGGTTGCAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCTTATGAAAACCAGTATCGGATCCGGC
TGCGCTGCGATGGCATCTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGTCGCCGTTTAGCCGCGCGGATTAAAATT
CTTTCCAAATTAGATATTGCCGAGCGCCGTTTGCCTCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGCCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAAATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCTACCCTGCATACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAATCTGGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.288

100

0.993
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.086

100

0.399
  pilF Thermus thermophilus HB27

39.372

96.263

0.379
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37