Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HPY08_RS02320 Genome accession   NZ_CP053808
Coordinates   483477..484352 (-) Length   291 a.a.
NCBI ID   WP_114716880.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478477..489352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY08_RS02300 (HPY08_02305) parC 479411..481696 (+) 2286 WP_000102510.1 DNA topoisomerase IV subunit A -
  HPY08_RS02305 (HPY08_02310) yacG 481745..481942 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY08_RS02310 (HPY08_02315) zapD 482100..482840 (-) 741 WP_000207194.1 cell division protein ZapD -
  HPY08_RS02315 (HPY08_02320) coaE 482872..483480 (-) 609 WP_162797106.1 dephospho-CoA kinase -
  HPY08_RS02320 (HPY08_02325) pilD 483477..484352 (-) 876 WP_114716880.1 A24 family peptidase Machinery gene
  HPY08_RS02325 (HPY08_02330) pilC 484410..485636 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY08_RS02330 (HPY08_02335) pilB 485679..487367 (-) 1689 WP_001960917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY08_RS02335 (HPY08_02340) pilA 487374..487850 (-) 477 WP_095473243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY08_RS02340 (HPY08_02345) nadC 488095..488985 (-) 891 WP_114716881.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32380.65 Da        Isoelectric Point: 6.6819

>NTDB_id=447643 HPY08_RS02320 WP_114716880.1 483477..484352(-) (pilD) [Vibrio cholerae strain SP7G]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLCQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=447643 HPY08_RS02320 WP_114716880.1 483477..484352(-) (pilD) [Vibrio cholerae strain SP7G]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCAAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTACCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGTGCTCGCTATCC
CCTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTACTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACTGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGTGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTGATTGATTGGTACTTCACCACTTGGGTAGGACAACCGCTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75

98.969

0.742
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443