Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY08_RS02335 Genome accession   NZ_CP053808
Coordinates   487374..487850 (-) Length   158 a.a.
NCBI ID   WP_095473243.1    Uniprot ID   -
Organism   Vibrio cholerae strain SP7G     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482374..492850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY08_RS02315 (HPY08_02320) coaE 482872..483480 (-) 609 WP_162797106.1 dephospho-CoA kinase -
  HPY08_RS02320 (HPY08_02325) pilD 483477..484352 (-) 876 WP_114716880.1 A24 family peptidase Machinery gene
  HPY08_RS02325 (HPY08_02330) pilC 484410..485636 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY08_RS02330 (HPY08_02335) pilB 485679..487367 (-) 1689 WP_001960917.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY08_RS02335 (HPY08_02340) pilA 487374..487850 (-) 477 WP_095473243.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY08_RS02340 (HPY08_02345) nadC 488095..488985 (-) 891 WP_114716881.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY08_RS02345 (HPY08_02350) ampD 489283..489828 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY08_RS02350 (HPY08_02355) fldB 489998..490516 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY08_RS02355 (HPY08_02360) xerD 490654..491562 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY08_RS02360 (HPY08_02365) dsbC 491579..492331 (+) 753 WP_071179237.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 16576.11 Da        Isoelectric Point: 7.6279

>NTDB_id=447646 HPY08_RS02335 WP_095473243.1 487374..487850(-) (pilA) [Vibrio cholerae strain SP7G]
MKAYKNKLQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLSEFPKALAAAKLAVEVCAHENASNETTFISNCVSGN
NGVPASFTLNNIDIQVITAANAGVSGAVAVEAKAKIAKGPIAQDESFIMAAVYKPEGLEWKSSCKDAAGKAQTSYCPD

Nucleotide


Download         Length: 477 bp        

>NTDB_id=447646 HPY08_RS02335 WP_095473243.1 487374..487850(-) (pilA) [Vibrio cholerae strain SP7G]
ATGAAAGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGCATTTT
GGCTGCCTTCGCCGTTCCGGCCTATCAAAACTATACAAAAAAAGCTACTTTATCTGAATTTCCAAAAGCACTAGCCGCAG
CCAAGTTAGCAGTCGAAGTATGCGCTCATGAGAATGCATCAAATGAAACAACCTTCATTTCAAATTGTGTTTCTGGCAAT
AATGGCGTTCCAGCGTCGTTTACACTAAACAATATCGACATTCAAGTAATAACAGCTGCCAATGCTGGCGTTTCAGGTGC
GGTGGCTGTTGAAGCCAAGGCAAAAATTGCTAAAGGCCCAATTGCCCAAGATGAAAGCTTCATTATGGCTGCTGTATATA
AGCCCGAAGGACTCGAATGGAAAAGTTCTTGTAAAGATGCAGCAGGTAAAGCTCAAACATCTTACTGCCCAGACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.688

99.367

0.563
  pilA Haemophilus influenzae 86-028NP

41.844

89.241

0.373
  pilA Haemophilus influenzae Rd KW20

42.446

87.975

0.373