Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY06_RS01320 Genome accession   NZ_CP053804
Coordinates   284320..285336 (+) Length   338 a.a.
NCBI ID   WP_175229780.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL6Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279320..290336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY06_RS01305 (HPY06_01310) - 279811..280542 (-) 732 WP_000495225.1 glutathione peroxidase -
  HPY06_RS01310 (HPY06_01315) oxyR 280682..281575 (+) 894 WP_001025911.1 DNA-binding transcriptional regulator OxyR -
  HPY06_RS01315 (HPY06_01320) - 281703..284177 (-) 2475 WP_175229778.1 penicillin-binding protein 1A -
  HPY06_RS01320 (HPY06_01325) pilM 284320..285336 (+) 1017 WP_175229780.1 type IV pilus assembly protein PilM Machinery gene
  HPY06_RS01325 (HPY06_01330) pilN 285326..285910 (+) 585 WP_175229782.1 PilN domain-containing protein Machinery gene
  HPY06_RS01330 (HPY06_01335) pilO 285903..286493 (+) 591 WP_175229784.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY06_RS01335 (HPY06_01340) pilP 286483..287001 (+) 519 WP_175231021.1 pilus assembly protein PilP Machinery gene
  HPY06_RS01340 (HPY06_01345) pilQ 287027..288742 (+) 1716 WP_175229786.1 type IV pilus secretin PilQ Machinery gene
  HPY06_RS01345 (HPY06_01350) aroK 288929..289453 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37667.37 Da        Isoelectric Point: 7.3328

>NTDB_id=447542 HPY06_RS01320 WP_175229780.1 284320..285336(+) (pilM) [Vibrio cholerae strain SL6Y]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGAQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRSLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447542 HPY06_RS01320 WP_175229780.1 284320..285336(+) (pilM) [Vibrio cholerae strain SL6Y]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCATGGTGCGCAAAGCCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCAACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCTTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCTCACTGGTTGATGGCCAGCGTTTTAGCACC
GCAGCCGGTCTTGCATTACGAGGCTTAGCATGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485