Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPY14_RS02330 Genome accession   NZ_CP053798
Coordinates   484135..485823 (-) Length   562 a.a.
NCBI ID   WP_175233820.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479135..490823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY14_RS02305 (HPY14_02305) yacG 480201..480398 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY14_RS02310 (HPY14_02310) zapD 480556..481296 (-) 741 WP_000207193.1 cell division protein ZapD -
  HPY14_RS02315 (HPY14_02315) coaE 481328..481936 (-) 609 WP_033928827.1 dephospho-CoA kinase -
  HPY14_RS02320 (HPY14_02320) pilD 481933..482808 (-) 876 WP_114720120.1 A24 family peptidase Machinery gene
  HPY14_RS02325 (HPY14_02325) pilC 482866..484092 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  HPY14_RS02330 (HPY14_02330) pilB 484135..485823 (-) 1689 WP_175233820.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY14_RS02335 (HPY14_02335) - 485830..486234 (-) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY14_RS02340 (HPY14_02340) nadC 486479..487369 (-) 891 WP_175233821.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY14_RS02345 (HPY14_02345) ampD 487671..488216 (+) 546 WP_000567317.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY14_RS02350 (HPY14_02350) fldB 488387..488905 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY14_RS02355 (HPY14_02355) xerD 489043..489951 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY14_RS02360 (HPY14_02360) dsbC 489968..490720 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62078.13 Da        Isoelectric Point: 6.0856

>NTDB_id=447411 HPY14_RS02330 WP_175233820.1 484135..485823(-) (pilB) [Vibrio cholerae strain SL5Y]
MLTNLVAILRQAELISATQEQAVITQVSASGTSVPEALLGLGIFHAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGSSDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=447411 HPY14_RS02330 WP_175233820.1 484135..485823(-) (pilB) [Vibrio cholerae strain SL5Y]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGATTACACAGGT
TAGCGCTTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACGCCCAAGAGCTGACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTCGCCGTCTCTGACCCGACCTTACT
GCAAGCTGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCGCTAGCCGATCACCGCGCACTGCAAGCGG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAACTGCAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGTAAAGGTTCCTCGGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATTTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCTGAGCGCCGTTTGCCTCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGCCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCAGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTGGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTCAAAGCCGCGCAAACTGGCCACTTGGTGCTTTCTACCCTGCACACCAACTCAGCCGCCGAAACCGT
GATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCCTCACTCAGTTTAATCATCGCCCAACGCCTTGCTC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTATATGAAGCCAATGCGGCGGGGTGTAACGAGTGCACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.288

100

0.993
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.488

100

0.735
  pilB Legionella pneumophila strain ERS1305867

51.619

93.416

0.482
  pilB Acinetobacter baylyi ADP1

50.29

91.993

0.463
  pilB Acinetobacter baumannii D1279779

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

45.374

100

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

37

100

0.395
  pilF Thermus thermophilus HB27

39.926

96.263

0.384
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37
  pilB Haemophilus influenzae Rd KW20

39.089

93.772

0.367