Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HPY14_RS02325 Genome accession   NZ_CP053798
Coordinates   482866..484092 (-) Length   408 a.a.
NCBI ID   WP_000648511.1    Uniprot ID   Q9X4G9
Organism   Vibrio cholerae strain SL5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477866..489092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY14_RS02300 (HPY14_02300) parC 477867..480152 (+) 2286 WP_000102516.1 DNA topoisomerase IV subunit A -
  HPY14_RS02305 (HPY14_02305) yacG 480201..480398 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY14_RS02310 (HPY14_02310) zapD 480556..481296 (-) 741 WP_000207193.1 cell division protein ZapD -
  HPY14_RS02315 (HPY14_02315) coaE 481328..481936 (-) 609 WP_033928827.1 dephospho-CoA kinase -
  HPY14_RS02320 (HPY14_02320) pilD 481933..482808 (-) 876 WP_114720120.1 A24 family peptidase Machinery gene
  HPY14_RS02325 (HPY14_02325) pilC 482866..484092 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  HPY14_RS02330 (HPY14_02330) pilB 484135..485823 (-) 1689 WP_175233820.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY14_RS02335 (HPY14_02335) - 485830..486234 (-) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY14_RS02340 (HPY14_02340) nadC 486479..487369 (-) 891 WP_175233821.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY14_RS02345 (HPY14_02345) ampD 487671..488216 (+) 546 WP_000567317.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY14_RS02350 (HPY14_02350) fldB 488387..488905 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45134.43 Da        Isoelectric Point: 10.3424

>NTDB_id=447410 HPY14_RS02325 WP_000648511.1 482866..484092(-) (pilC) [Vibrio cholerae strain SL5Y]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=447410 HPY14_RS02325 WP_000648511.1 482866..484092(-) (pilC) [Vibrio cholerae strain SL5Y]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTGTCTCTTTTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGCCAGTTAGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCAATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTCGAAACCGGAGAGATGTCCGGTAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCCGAGCTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTACGTAAAAACTCCTTCCAGATCCGTTTAAAAACCAGTCGATTAGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACAGTCGATAACTTGG
GCAAGATTCTTGAGCCGCTGATCATCGTGTTTCTTGGGACAGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9X4G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

100

100

1
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402