Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY12_RS02345 Genome accession   NZ_CP053794
Coordinates   485955..486374 (-) Length   139 a.a.
NCBI ID   WP_057567630.1    Uniprot ID   -
Organism   Vibrio cholerae strain W10G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480955..491374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY12_RS02325 (HPY12_02325) coaE 481453..482061 (-) 609 WP_057567633.1 dephospho-CoA kinase -
  HPY12_RS02330 (HPY12_02330) pilD 482058..482933 (-) 876 WP_057567632.1 A24 family peptidase Machinery gene
  HPY12_RS02335 (HPY12_02335) pilC 482991..484217 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY12_RS02340 (HPY12_02340) pilB 484260..485948 (-) 1689 WP_057567631.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY12_RS02345 (HPY12_02345) pilA 485955..486374 (-) 420 WP_057567630.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY12_RS02350 (HPY12_02350) nadC 486619..487509 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY12_RS02355 (HPY12_02355) ampD 487807..488352 (+) 546 WP_057567629.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY12_RS02360 (HPY12_02360) fldB 488473..488991 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY12_RS02365 (HPY12_02365) xerD 489129..490037 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY12_RS02370 (HPY12_02370) dsbC 490054..490806 (+) 753 WP_175234136.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14364.57 Da        Isoelectric Point: 6.2547

>NTDB_id=447316 HPY12_RS02345 WP_057567630.1 485955..486374(-) (pilA) [Vibrio cholerae strain W10G]
MKAYKNKLQKGFTLIELMIVVAIIGVLAAVAIPAYQDYVKKSEAASALATLRALITPAELFYQEKGVGAAATVAELGSSG
TANDLGTITSALVNAKPTLKFEFGANSSMASTDTLTFTRDTGGWTCAIAGNVPAIDGCQ

Nucleotide


Download         Length: 420 bp        

>NTDB_id=447316 HPY12_RS02345 WP_057567630.1 485955..486374(-) (pilA) [Vibrio cholerae strain W10G]
ATGAAAGCGTATAAAAACAAGCTACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTAGTGGCGATTATTGGTGTGTT
GGCTGCTGTCGCTATTCCGGCTTATCAAGATTATGTAAAAAAATCGGAGGCTGCTTCTGCTTTAGCCACATTAAGAGCAT
TGATCACTCCTGCTGAGCTGTTCTATCAAGAAAAAGGAGTTGGAGCCGCAGCTACCGTCGCCGAATTAGGTTCATCAGGT
ACTGCCAATGATCTAGGGACTATTACATCCGCTCTAGTCAATGCTAAACCAACCCTTAAATTTGAGTTTGGTGCCAATAG
CTCGATGGCAAGCACTGACACATTAACTTTTACTAGAGATACTGGTGGCTGGACATGTGCAATAGCCGGTAATGTTCCAG
CTATTGATGGTTGCCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

62.59

100

0.626
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.043

100

0.46
  pilA Vibrio cholerae strain A1552

46.043

100

0.46
  pilA Vibrio cholerae C6706

46.043

100

0.46
  pilA Pseudomonas aeruginosa PAK

47.015

96.403

0.453
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

94.964

0.396
  pilA/pilA1 Eikenella corrodens VA1

39.85

95.683

0.381