Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FX982_RS10960 Genome accession   NZ_CP053746
Coordinates   2425002..2426219 (-) Length   405 a.a.
NCBI ID   WP_172610647.1    Uniprot ID   A0A6M8MM98
Organism   Pseudomonas graminis strain PgKB30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2420002..2431219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS10930 (FX982_02188) - 2420365..2421318 (+) 954 WP_172610643.1 2-hydroxyacid dehydrogenase -
  FX982_RS10935 (FX982_02189) - 2421507..2422406 (-) 900 WP_172610644.1 LysR family transcriptional regulator -
  FX982_RS10940 (FX982_02190) - 2422563..2423207 (+) 645 WP_122535388.1 hydrolase -
  FX982_RS10945 (FX982_02191) yacG 2423307..2423510 (-) 204 WP_062378727.1 DNA gyrase inhibitor YacG -
  FX982_RS10950 (FX982_02192) coaE 2423510..2424130 (-) 621 WP_172610645.1 dephospho-CoA kinase -
  FX982_RS10955 (FX982_02193) pilD 2424127..2424999 (-) 873 WP_172610646.1 A24 family peptidase Machinery gene
  FX982_RS10960 (FX982_02194) pilC 2425002..2426219 (-) 1218 WP_172610647.1 type II secretion system F family protein Machinery gene
  FX982_RS10965 (FX982_02195) pilB 2426222..2427916 (-) 1695 WP_172610648.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FX982_RS10970 (FX982_02196) - 2428141..2428569 (+) 429 WP_172610649.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FX982_RS10980 (FX982_02199) nadC 2429005..2429853 (-) 849 WP_172610650.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44284.31 Da        Isoelectric Point: 10.0294

>NTDB_id=446828 FX982_RS10960 WP_172610647.1 2425002..2426219(-) (pilC) [Pseudomonas graminis strain PgKB30]
MATKAVKVSVYAWEGLDKKGTKLTGETTGHNPALVKALLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGHDNPNMRKLVDDIKQEVAAGHSFATALRQKPLYFDDLFCNLVDAGEQAGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVVIVAFIVTGILLLKVVPQFQTIFEGFGAELPAFTLMVIGLSKIVQDYWWMILAGIGLSIFLFR
RAYRTSEKFRNGLDRALLKVPVIGPLLYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPMLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAFLGVIVGGLVIAMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=446828 FX982_RS10960 WP_172610647.1 2425002..2426219(-) (pilC) [Pseudomonas graminis strain PgKB30]
ATGGCGACCAAAGCAGTAAAAGTCAGCGTCTATGCTTGGGAAGGGCTGGATAAAAAAGGGACAAAGCTAACCGGCGAAAC
AACTGGGCACAACCCTGCTCTGGTCAAGGCCTTACTCCGCAAGCAGGGCATCAACCCAACCAAGGTCCGTAAAAAATCCG
TTTCGATCTTTGGCAAGGGTAAAAAGATTAAGCCGCTGGACATCGCATTCTTCAGCCGCCAGATGGCCACCATGATGAAA
GCGGGTGTACCGCTCCTACAATCCTTCGACATCATCAGCGAGGGGCATGACAACCCGAACATGCGCAAGTTGGTAGACGA
CATCAAACAGGAAGTCGCGGCAGGGCACAGCTTTGCTACAGCACTGAGGCAGAAGCCCTTATATTTCGACGACCTTTTCT
GCAATCTGGTAGACGCTGGCGAACAAGCGGGGGCTCTTGAAACTTTGTTGGATCGAGTCGCGACCTACAAAGAAAAGACC
GAATCGCTTAAAGCCAAGATCAAAAAAGCGATGACCTATCCCATCGCTGTGGTCATCGTCGCCTTTATCGTAACGGGGAT
TTTGTTACTCAAAGTAGTACCCCAGTTTCAGACTATTTTTGAAGGGTTTGGAGCGGAGCTACCTGCATTCACATTGATGG
TGATCGGCTTATCAAAAATTGTTCAGGACTATTGGTGGATGATACTGGCCGGGATCGGATTAAGTATTTTCTTGTTTCGG
CGCGCGTACCGAACTTCCGAAAAGTTTAGAAATGGCCTGGACCGGGCACTGCTCAAGGTGCCTGTCATCGGTCCGTTGTT
GTACAAATCCGCCGTGGCGCGATATGCGCGGACTTTGTCCACGACATTCGCTGCGGGCGTTCCACTGGTAGAGGCGCTTG
ATTCCGTCGCTGGCGCGACCGGGAATGTTGTTTTCAAGAACGCCGTCAACAAAGTCAAGCAGGACGTTTCCACCGGCATG
CAACTCAACTTCTCCATGCGCAGTACCGGCGTGTTTCCGATGCTGGCCATACAGATGACCGCAATTGGAGAGGAATCCGG
AGCGCTGGACTCGATGCTTGATAAAGTCGCGACCTACTATGAGGACGAAGTTGACAATATGGTCGACAGCCTGACGAGCC
TTATGGAACCCATGATTATGGCTTTCTTGGGTGTCATTGTCGGCGGACTCGTGATCGCCATGTACCTCCCTATTTTCAAG
CTCGGACAAGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8MM98

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.049

100

0.76
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilC Vibrio campbellii strain DS40M4

41.667

100

0.42
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilG Neisseria meningitidis 44/76-A

42.04

99.259

0.417
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilC Thermus thermophilus HB27

38.903

99.012

0.385