Detailed information NTG0446827

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FX982_RS10955
• Genome accession: NZ_CP053746
• Coordinates: 2424127..2424999 (-)
• Length: 290 a.a.
• NCBI ID: WP_172610646.1
• Uniprot ID: A0A6M8MNE9
• Organism: Pseudomonas graminis strain PgKB30
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2419127..2429999

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FX982_RS10925 (FX982_02187)	-	2419569..2420333 (+)	765	WP_122535385.1	SDR family oxidoreductase	-
FX982_RS10930 (FX982_02188)	-	2420365..2421318 (+)	954	WP_172610643.1	2-hydroxyacid dehydrogenase	-
FX982_RS10935 (FX982_02189)	-	2421507..2422406 (-)	900	WP_172610644.1	LysR family transcriptional regulator	-
FX982_RS10940 (FX982_02190)	-	2422563..2423207 (+)	645	WP_122535388.1	hydrolase	-
FX982_RS10945 (FX982_02191)	yacG	2423307..2423510 (-)	204	WP_062378727.1	DNA gyrase inhibitor YacG	-
FX982_RS10950 (FX982_02192)	coaE	2423510..2424130 (-)	621	WP_172610645.1	dephospho-CoA kinase	-
FX982_RS10955 (FX982_02193)	pilD	2424127..2424999 (-)	873	WP_172610646.1	A24 family peptidase	Machinery gene
FX982_RS10960 (FX982_02194)	pilC	2425002..2426219 (-)	1218	WP_172610647.1	type II secretion system F family protein	Machinery gene
FX982_RS10965 (FX982_02195)	pilB	2426222..2427916 (-)	1695	WP_172610648.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FX982_RS10970 (FX982_02196)	-	2428141..2428569 (+)	429	WP_172610649.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
FX982_RS10980 (FX982_02199)	nadC	2429005..2429853 (-)	849	WP_172610650.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32040.37 Da
• Isoelectric Point: 7.6016

>NTDB_id=446827 FX982_RS10955 WP_172610646.1 2424127..2424999(-) (pilD) [Pseudomonas graminis strain PgKB30]
MPLLDLLASSPLAFVLCALVFGLLVGSFLNVVIYRLPNMMIRDWKIQSREMLDLPAEPQPETFNLILPHSRCPHCSHKIR
AWENIPVVSYLFLGGKCSQCKAPISKRYPLVELACGLLSAFIAWHFGFGWQAGAMLVLTWGLLAMSLIDADHQLLPDAIV
LPLLWLGLIVNAFGLFTSLTDALWGAVAGYLVLWCVFWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSIVG
AVLGLILLRLRNVETSTPIPFGPYLALAGWIALLWGDQITTSYLQIAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=446827 FX982_RS10955 WP_172610646.1 2424127..2424999(-) (pilD) [Pseudomonas graminis strain PgKB30]
ATGCCCCTCCTCGACCTCCTGGCCAGTTCCCCGCTGGCCTTCGTTCTATGCGCACTCGTCTTTGGCCTGCTCGTCGGCAG
TTTCCTCAACGTGGTCATCTACCGCCTCCCCAACATGATGATCCGCGACTGGAAAATCCAGTCCCGGGAAATGCTCGACC
TCCCCGCCGAGCCGCAACCAGAAACCTTCAACCTCATCCTCCCCCACTCCCGCTGCCCGCACTGCTCACACAAAATCCGC
GCGTGGGAGAACATTCCGGTCGTGAGTTATCTCTTCCTCGGCGGCAAGTGTTCCCAGTGCAAAGCCCCCATCAGCAAGCG
CTACCCGCTGGTTGAATTGGCGTGCGGCTTGCTGTCGGCCTTCATCGCCTGGCATTTCGGTTTCGGCTGGCAGGCTGGGG
CGATGCTGGTGCTGACATGGGGTTTGTTGGCGATGAGTCTGATCGACGCCGACCACCAGCTTCTGCCGGACGCAATCGTA
TTGCCGTTGTTGTGGCTGGGCCTGATCGTGAATGCGTTCGGGCTGTTCACGTCGCTGACCGATGCGCTGTGGGGCGCGGT
CGCGGGGTATCTGGTGTTGTGGTGTGTGTTCTGGCTGTTCAAGCTGATCACGGGCAAGGAAGGCATGGGTTACGGCGACT
TCAAGTTGCTGGCGATGCTCGGCGCGTGGGGTGGGTGGCAGATTTTGCCGCTGACGATTCTGCTGTCGTCGATTGTCGGG
GCGGTTTTGGGGCTGATCCTGCTGCGTTTGCGCAATGTCGAGACCAGCACGCCGATCCCCTTCGGTCCTTATCTGGCCCT
CGCGGGGTGGATCGCTTTGCTCTGGGGTGATCAAATAACCACTTCGTATTTGCAGATCGCCGGCTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A6M8MNE9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.357	97.586	0.521
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	54.212	94.138	0.51
pilD	Acinetobacter nosocomialis M2	53.846	94.138	0.507
pilD	Neisseria gonorrhoeae MS11	50	95.172	0.476