Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FX982_RS10965 Genome accession   NZ_CP053746
Coordinates   2426222..2427916 (-) Length   564 a.a.
NCBI ID   WP_172610648.1    Uniprot ID   A0A6M8MMH2
Organism   Pseudomonas graminis strain PgKB30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2421222..2432916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FX982_RS10935 (FX982_02189) - 2421507..2422406 (-) 900 WP_172610644.1 LysR family transcriptional regulator -
  FX982_RS10940 (FX982_02190) - 2422563..2423207 (+) 645 WP_122535388.1 hydrolase -
  FX982_RS10945 (FX982_02191) yacG 2423307..2423510 (-) 204 WP_062378727.1 DNA gyrase inhibitor YacG -
  FX982_RS10950 (FX982_02192) coaE 2423510..2424130 (-) 621 WP_172610645.1 dephospho-CoA kinase -
  FX982_RS10955 (FX982_02193) pilD 2424127..2424999 (-) 873 WP_172610646.1 A24 family peptidase Machinery gene
  FX982_RS10960 (FX982_02194) pilC 2425002..2426219 (-) 1218 WP_172610647.1 type II secretion system F family protein Machinery gene
  FX982_RS10965 (FX982_02195) pilB 2426222..2427916 (-) 1695 WP_172610648.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FX982_RS10970 (FX982_02196) - 2428141..2428569 (+) 429 WP_172610649.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FX982_RS10980 (FX982_02199) nadC 2429005..2429853 (-) 849 WP_172610650.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FX982_RS10985 (FX982_02200) - 2430238..2432478 (+) 2241 WP_172610651.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62289.66 Da        Isoelectric Point: 5.9477

>NTDB_id=446829 FX982_RS10965 WP_172610648.1 2426222..2427916(-) (pilB) [Pseudomonas graminis strain PgKB30]
MTDVVLTGLAKQLVVAELLTEKTAQKAYEQARRDKVSLVHHLVETKLLKSITLAEVASDQFGIPFLDLGTLDKETQPKGL
VSEKLIRQHCALPLWRRGNKLFVGISDPTNHQAITDIQFSTGLTTEAILVEDDKLTFAIDKFFDGNAGMGDLADVDLGLE
IEAVDESKETAIGGQDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPIHLAGRIAARLKV
MASLDISERRKPQDGRIKLRISKNRAIDFRVNTLPTLWGEKIVMRILDPTTAQMGIDALGYEPEQKALYLEALRQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCAHCKKEVEIPRETLIAEGFPESQ
IGSFQLYSPVGCEHCNGGYKGRVGIYEVVKSTPALERIIMEEGNSIQISEQMRRDGFNDLRTSGLMKAMQGVTSLEEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=446829 FX982_RS10965 WP_172610648.1 2426222..2427916(-) (pilB) [Pseudomonas graminis strain PgKB30]
ATGACTGACGTCGTCCTAACCGGCTTGGCTAAACAACTTGTTGTGGCTGAGCTGCTCACTGAAAAAACGGCCCAAAAAGC
CTACGAACAAGCGCGACGTGACAAGGTTTCGCTCGTGCACCACCTCGTCGAGACGAAGCTGCTTAAGAGCATCACGCTGG
CTGAGGTCGCGTCAGATCAATTCGGCATTCCATTTCTTGATCTCGGCACGCTGGACAAGGAGACCCAACCGAAAGGGCTC
GTCAGTGAGAAGCTCATTCGCCAGCACTGCGCGCTTCCGCTGTGGCGTAGGGGTAACAAGCTTTTTGTGGGCATATCCGA
CCCCACCAATCATCAAGCTATTACTGACATCCAGTTCAGCACCGGCCTGACCACTGAAGCCATATTGGTCGAAGACGACA
AACTGACCTTCGCCATCGATAAGTTTTTCGACGGCAATGCTGGTATGGGCGACTTGGCGGACGTTGACCTTGGTCTAGAA
ATCGAAGCGGTCGATGAGAGCAAAGAAACTGCGATTGGCGGCCAAGACGCCGATGACGCGCCGGTCGTCCGCTTCGTTAA
TAAGATGCTGATGGACGCTATCCGCCTAGGATCGTCTGATCTGCATTTCGAACCCTATGAAAAGATCTTTCGTGTAAGGC
TCCGCACCGACGGCATTCTTCATGAGGTGGCAAAGCCGCCCATTCACTTGGCTGGCCGGATCGCTGCACGCCTGAAGGTC
ATGGCAAGCCTCGATATTTCGGAGCGTCGAAAACCGCAAGATGGCCGCATCAAACTCCGAATTTCTAAAAACCGGGCCAT
CGACTTCCGGGTCAACACGCTACCCACACTGTGGGGCGAAAAAATCGTGATGCGGATTCTGGATCCCACTACCGCTCAAA
TGGGCATTGACGCCTTAGGCTACGAGCCAGAACAGAAAGCGCTTTACCTTGAAGCTCTTCGCCAACCCCAAGGCATGATC
CTGGTGACCGGCCCTACCGGCTCGGGTAAAACAGTATCGCTCTACACTGGATTGAATATTCTCAACACTGTCGATATCAA
TATATCGACGGCAGAGGACCCGGTCGAAATAAATCTCGAAGGCATCAACCAGGTCAACGTCAATCCGCGTCAGGGGATGG
ACTTCTCTCAGGCACTACGAGCGTTTCTGCGTCAGGATCCTGACGTAATCATGGTGGGTGAAATCCGAGATCTGGAAACA
GCCGAAATCGCGATTAAGGCTTCGCAGACCGGCCACATGGTTTTGTCTACGCTGCACACCAACAGCGCGGCTGAAACCCT
GACTCGCCTGCACCACATGGGCGTTGCAGCGTTCAACATTGCGACCGCAATCAACCTGATCATTGCTCAACGCCTGGCGC
GCAAACTGTGCGCGCACTGCAAGAAAGAAGTCGAGATCCCACGGGAGACATTGATTGCAGAAGGTTTCCCCGAGTCGCAA
ATCGGATCATTCCAGCTTTACTCCCCAGTGGGATGCGAGCACTGCAACGGCGGCTACAAAGGCCGAGTAGGTATCTACGA
AGTGGTGAAGAGCACGCCCGCACTGGAGCGCATCATCATGGAGGAAGGCAACTCGATCCAGATTTCCGAACAGATGCGCA
GAGACGGCTTCAACGATCTGCGCACGTCCGGCCTGATGAAGGCGATGCAGGGCGTAACCAGTCTTGAAGAAATAAACCGG
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8MMH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.848

100

0.582
  pilB Acinetobacter baylyi ADP1

56.762

99.645

0.566
  pilB Legionella pneumophila strain ERS1305867

54.417

100

0.546
  pilB Vibrio cholerae strain A1552

51.418

100

0.514
  pilB Vibrio campbellii strain DS40M4

49.037

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.551

98.759

0.489
  pilF Neisseria gonorrhoeae MS11

48.932

99.645

0.488
  pilF Thermus thermophilus HB27

37.809

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.544

93.262

0.369