Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY04_RS02395 Genome accession   NZ_CP053744
Coordinates   501801..502232 (-) Length   143 a.a.
NCBI ID   WP_057563244.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA3G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496801..507232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY04_RS02375 (HPY04_02375) coaE 497304..497912 (-) 609 WP_032479520.1 dephospho-CoA kinase -
  HPY04_RS02380 (HPY04_02380) pilD 497909..498784 (-) 876 WP_175248719.1 A24 family peptidase Machinery gene
  HPY04_RS02385 (HPY04_02385) pilC 498842..500068 (-) 1227 WP_175248720.1 type II secretion system F family protein Machinery gene
  HPY04_RS02390 (HPY04_02390) pilB 500111..501799 (-) 1689 WP_175248721.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY04_RS02395 (HPY04_02395) pilA 501801..502232 (-) 432 WP_057563244.1 pilin Machinery gene
  HPY04_RS02400 (HPY04_02400) nadC 502477..503367 (-) 891 WP_000665277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY04_RS02405 (HPY04_02405) ampD 503669..504214 (+) 546 WP_000567314.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY04_RS02410 (HPY04_02410) fldB 504363..504881 (-) 519 WP_019828645.1 flavodoxin FldB -
  HPY04_RS02415 (HPY04_02415) xerD 505019..505927 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY04_RS02420 (HPY04_02420) dsbC 505944..506696 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14653.68 Da        Isoelectric Point: 9.4343

>NTDB_id=446776 HPY04_RS02395 WP_057563244.1 501801..502232(-) (pilA) [Vibrio cholerae strain SA3G]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPQYQKYVAKSEAAAALASITGHRTNVETYVLENGSFPTSSAVPVPSTS
TGAITYSSGASGAGSIIFTFAASGASPSVVTKNVTLARDGDGKWICTSSIGSDFRPKGCAASQ

Nucleotide


Download         Length: 432 bp        

>NTDB_id=446776 HPY04_RS02395 WP_057563244.1 501801..502232(-) (pilA) [Vibrio cholerae strain SA3G]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGCGTGTT
GGCTGCCATTGCAGTACCTCAGTACCAAAAATACGTTGCGAAAAGTGAAGCAGCAGCAGCACTGGCTTCAATCACCGGGC
ATAGAACAAACGTTGAAACATACGTATTGGAAAATGGTTCGTTCCCAACATCATCTGCCGTGCCTGTTCCATCAACAAGT
ACAGGAGCAATTACTTACAGCTCAGGGGCATCTGGTGCAGGCAGCATCATCTTCACATTTGCAGCCAGTGGTGCAAGCCC
TAGCGTTGTGACTAAAAACGTCACTTTAGCTCGAGACGGAGATGGCAAATGGATCTGTACATCTTCTATTGGCTCAGATT
TCAGACCCAAAGGCTGTGCGGCCTCCCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.658

100

0.538
  pilA Vibrio cholerae strain A1552

50.658

100

0.538
  pilA Vibrio cholerae C6706

50.658

100

0.538
  pilA Pseudomonas aeruginosa PAK

45.946

100

0.476
  pilA Acinetobacter baumannii strain A118

47.482

97.203

0.462
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.254

99.301

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.845

99.301

0.406
  pilA Vibrio parahaemolyticus RIMD 2210633

44.615

90.909

0.406
  comP Acinetobacter baylyi ADP1

37.762

100

0.378