Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HOO69_RS00790 Genome accession   NZ_CP053541
Coordinates   144244..144678 (-) Length   144 a.a.
NCBI ID   WP_069667590.1    Uniprot ID   A0A178JAH3
Organism   Vibrio europaeus strain NPI-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 139244..149678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HOO69_RS00770 (HOO69_00775) coaE 139776..140384 (-) 609 WP_069667594.1 dephospho-CoA kinase -
  HOO69_RS00775 (HOO69_00780) pilD 140386..141255 (-) 870 WP_171801081.1 A24 family peptidase Machinery gene
  HOO69_RS00780 (HOO69_00785) pilC 141313..142536 (-) 1224 WP_171801082.1 type II secretion system F family protein Machinery gene
  HOO69_RS00785 (HOO69_00790) pilB 142547..144238 (-) 1692 WP_171801083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HOO69_RS00790 (HOO69_00795) pilA 144244..144678 (-) 435 WP_069667590.1 pilin Machinery gene
  HOO69_RS00795 (HOO69_00800) nadC 144921..145808 (-) 888 WP_069667589.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HOO69_RS00800 (HOO69_00805) ampD 145940..146494 (+) 555 WP_171801084.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HOO69_RS00805 (HOO69_00810) fldB 146772..147293 (-) 522 WP_171801085.1 flavodoxin FldB -
  HOO69_RS00810 (HOO69_00815) xerD 147456..148352 (+) 897 WP_171802358.1 site-specific tyrosine recombinase XerD -
  HOO69_RS00815 (HOO69_00820) - 148393..149145 (+) 753 WP_171382778.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14567.37 Da        Isoelectric Point: 4.4295

>NTDB_id=445041 HOO69_RS00790 WP_069667590.1 144244..144678(-) (pilA) [Vibrio europaeus strain NPI-1]
MQSSQSSKAKGFTLIELMIVVAIIGVLASISIPAYQDYVRKSEAASALATLKSLITPAEVFYQENGTASAAALSNLGIDS
GANNLGTIASAITGTGSAAVPTLKFTFDSDSSMGSSDTITYSRDPSTGWSCAKAGAVPSLDGCS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=445041 HOO69_RS00790 WP_069667590.1 144244..144678(-) (pilA) [Vibrio europaeus strain NPI-1]
ATGCAAAGTTCACAAAGCAGCAAAGCCAAAGGCTTTACCTTAATTGAGTTGATGATTGTGGTAGCAATTATTGGCGTGCT
TGCGTCGATATCAATACCAGCTTATCAAGACTATGTTAGGAAATCTGAGGCGGCTTCAGCGCTAGCGACGTTAAAAAGCC
TGATTACGCCAGCTGAAGTTTTCTATCAAGAAAACGGCACCGCATCGGCCGCCGCTTTATCTAATTTAGGCATCGATAGC
GGCGCTAATAATTTAGGAACCATTGCCTCAGCAATAACTGGAACAGGTTCAGCAGCTGTTCCAACATTAAAGTTCACATT
CGATTCCGACAGCTCAATGGGAAGCAGTGACACCATTACCTATTCTCGAGACCCGAGTACCGGTTGGTCTTGCGCAAAAG
CGGGAGCAGTCCCTTCTCTAGATGGATGCTCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A178JAH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

59.028

100

0.59
  pilA Pseudomonas aeruginosa PAK

44.275

90.972

0.403
  pilA Vibrio cholerae strain A1552

40.58

95.833

0.389
  pilA Vibrio cholerae C6706

40.58

95.833

0.389
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.58

95.833

0.389
  comP Acinetobacter baylyi ADP1

38.406

95.833

0.368
  pilA/pilA1 Eikenella corrodens VA1

38.519

93.75

0.361